Alterations of glutamine and glutamate levels in patients and rats with hepatocellular carcinoma

Yang, Wenlong; Zhu, Guojian; Zhou, Guanlin; Liu, Yan; Shao, Jianghua; Xie, Baogang

doi:10.1080/10826076.2018.1485034

Cited by 1 publication

(2 citation statements)

References 18 publications

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“…MassLynx4.1(Waters, USA) software was used to control instrumentation and data processing. The method validation procedure for measuring serum amino acids has been described in our previous paper (Yang et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

“…The electrospray capillary voltage was set to 3 kV, nitrogen was used as a dry gas for solvent evaporation, the flow rate was 50 L/h and the ion source temperature was 100 C. MassLynx4.1(Waters, USA) software was used to control instrumentation and data processing. The method validation procedure for measuring serum amino acids has been described in our previous paper (Yang et al, 2018).…”

Section: Quantitative Analysis Of Metabolites By Uplc/q-tof Ms/msmentioning

confidence: 99%

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Metabolomics strategy of Pikang oral liquid in the treatment of psoriasis

Wang

Xie

Lan

et al. 2023

Biomedical Chromatography

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This study aimed to investigate the effect of Pikang oral liquid (PK) on psoriasis and analyze its possible mechanism from the perspective of metabolism. A psoriasis‐form mouse model established using imiquimod (IMQ) was used to evaluate the anti‐psoriatic effects of PK. The serum samples were analyzed by high‐resolution nuclear magnetic resonance (1H NMR)‐based metabonomics. Nine amino acids were further quantitatively analyzed by ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry (UPLC/Q‐TOF MS). This study suggested that PK treatment markedly attenuated IMQ‐induced psoriasis in a dose‐dependent manner. 1H NMR‐based multivariate trajectory analysis revealed that PK had a certain regression effect on eight differential metabolites. The quantitative results showed that PK could significantly regulate the serum levels of alanine, histidine and arginine to healthy control levels. The anti‐psoriasis mechanism of PK may be associated with the restoration of the disturbance in the amino acid metabolism, energy metabolism and lipid metabolism and so on. Quantitative results further confirmed that amino acid metabolism play an key role in the pathogenesis of psoriasis. Our investigation provided a holistic view of PK for intervention psoriasis and provided the scientific information in vivo about a clinical value of PK for psoriasis.

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Section: Methodsmentioning

confidence: 99%

Section: Quantitative Analysis Of Metabolites By Uplc/q-tof Ms/msmentioning

confidence: 99%