1982
DOI: 10.1128/mcb.2.11.1459
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Alterations of Gene Structure in Ethyl Methane Sulfonate-Induced Mutants of Mammalian Cells

Abstract: To determine the types of alterations in gene structure induced by DNA-alkylating agents, we analyzed the restriction enzyme cleavage patterns of adenine phosphoribosyltransferase gene sequences in mutant strains of Chinese hamster ovary cells deficient in this enzyme. Base pair changes as detected by loss of restriction enzyme sites were found, but no major internal gene rearrangements could be detected.

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Cited by 44 publications
(22 citation statements)
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“…Breimer et al (1986), Grosovsky et al (1986fFuscoe et al (1983), Vrieling et al (1985, Breimer et al (1986), Fuscoe et al (1986), 3Skulimowski et al (1986), Liber et al (1987hYandell et al (1986hYandell et al ( , 1987 aprt locus The endogenous genetic locus coding for the non-essential enzyme adenine phosphoribosyl transferase (aprt) in cultured Chinese hamster ovary cells is particularly attractive for analysis of mutational events because it offers a much greater resolution than any other mammalian locus used so far (Meuth & Arrand, 1982;Nalbantoglu et al, 1983). The locus is small (3.9 kb) and contains a large number of restriction endonuclease recognition sites closely spaced, allowing detection and mapping of alterations as small as 25bp.…”
mentioning
confidence: 99%
“…Breimer et al (1986), Grosovsky et al (1986fFuscoe et al (1983), Vrieling et al (1985, Breimer et al (1986), Fuscoe et al (1986), 3Skulimowski et al (1986), Liber et al (1987hYandell et al (1986hYandell et al ( , 1987 aprt locus The endogenous genetic locus coding for the non-essential enzyme adenine phosphoribosyl transferase (aprt) in cultured Chinese hamster ovary cells is particularly attractive for analysis of mutational events because it offers a much greater resolution than any other mammalian locus used so far (Meuth & Arrand, 1982;Nalbantoglu et al, 1983). The locus is small (3.9 kb) and contains a large number of restriction endonuclease recognition sites closely spaced, allowing detection and mapping of alterations as small as 25bp.…”
mentioning
confidence: 99%
“…Other endpoints have, by necessity, been used to construct a preliminary view of mutation in mammalian cells. These include comparative mutation induction at several selectable loci (4,5), twodimensional gel electrophoresis analysis of mutant proteins (6), and Southern blot analysis of restriction site polymorphisms (7)(8)(9)(10). Recently, recombinant shuttle vectors, which can be propagated in both prokaryotic and eukaryotic cells, have been developed for the analysis of mutant DNA sequences in mammalian cells (reviewed in ref.…”
mentioning
confidence: 99%
“…Ethyl methane sulphonate is a monofunctional alkylating agent and is considered as a potent mutagen and carcinogen (Meuth and Arrand 1982). In plants, EMS chemically alters DNA through the modification of oxygen present in nucleotide bases as well as in DNA phosphate groups (Sega 1984).…”
mentioning
confidence: 99%