Alterations of estrogen receptor-α and -β in the anterior vaginal wall of women with urinary incontinence

Xie, Zhenwei; Shi, Haiyan; Zhou, Caiyun; Dong, Minyue; Liang, Hong; Jin, Hong-Ying

doi:10.1016/j.ejogrb.2006.10.039

Cited by 29 publications

(18 citation statements)

References 23 publications

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“…Our result confirm previous studies reporting a decrease of ERb in the vaginal stroma but not in the mucosa. 29 The unchanged ERb expression at the mucosal level may be an attempt to partially compensate ERa downregulation, as suggested also by experimental data conducted on mutant mice lacking ERa, ERb or both. 30 No data in the literature exists on the regulation of ERb by T. The decrease that we see in our samples may be related to the reduced circulating E levels following T administration or to a direct effect of T. Taken together, these observations indicate that ER isoforms are differently distributed and regulated by sex steroid hormones (E and T) at the vaginal level.…”

Section: Discussionmentioning

confidence: 94%

Effects of long-term high dose testosterone administration on vaginal epithelium structure and estrogen receptor-α and -β expression of young women

et al. 2013

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To date, the effects of long-term testosterone (T) administration on the human vagina are not completely understood. Thus, the aim of this study was to investigate the effects of long-term T treatment on vaginal tissue histology, estrogen receptor alpha (ERα) and beta (ERβ) expression and proliferation in female to male transsexual subjects (FtM). We compared vaginal samples from FtM subjects with those of premenopausal women (PrM) and postmenopausal women (M) not receiving any hormonal treatment for at least 2 years. Vaginal tissue samples from 16 FtM subjects treated with T (intramuscular injections of 100 mg Testoviron Depot/7-10 days for at least 1 year), undergoing sex reassignment surgery, and 16 PrM and 16 M subjects undergoing a vaginal hysterectomy for prolapse, were collected. For each sample, morphology, glycogen content, proliferation (ki-67), ERα and ERβ expression were evaluated. Vaginal samples from FtM showed a loss of normal architecture of the epithelium, intermediate and superficial layers were completely lost, and glycogen content was depleted. T administration resulted in a strong proliferation reduction when compared with both M and PrM subjects. Stromal and epithelial ERα as well as ERβ were significantly decreased in FtM when compared with PrM subjects. In conclusion, our data suggests that systemic T administration at supraphysiological dosage, determines profound changes in histomorphology and reduces ERs expression and proliferation of vaginal epithelium.

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Section: Discussionmentioning

confidence: 94%

Effects of long-term high dose testosterone administration on vaginal epithelium structure and estrogen receptor-α and -β expression of young women

et al. 2013

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“…Morphometric and functional alterations in these muscles are related to the onset of gynaecological and urinary dysfunctions in women, some of them modelled in experimental animals . The variation in the expression of estrogen receptors (ER) in the lower urogenital tract has been associated with some pelvic dysfunctions . Estrogen replacement therapy is commonly prescribed for the treatment of pelvic dysfunctions; however, its efficacy is controversial .…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4] The variation in the expression of estrogen receptors (ER) in the lower urogenital tract has been associated with some pelvic dysfunctions. [4][5][6][7][8] Estrogen replacement therapy is commonly prescribed for the treatment of pelvic dysfunctions; however, its efficacy is controversial. 9 The effect of the estrogen administration on periurethral skeletal muscles has been previously approached, 6,8,10,11 although some issues regarding their estrogenic sensitivity remain to be addressed.…”

Section: Introductionmentioning

confidence: 99%

Aromatase expression is linked to estrogenic sensitivity of periurethral muscles in female rabbits

Carrasco-Ruiz

García-Villamar

López-García

et al. 2015

Cell Biochemistry & Function

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Beyond its role in the conversion of androgens to estrogens, the expression of aromatase could influence on the estrogenic signalling in targeted tissues. Considering the well-defined biochemical and physiological differences between the pubococcygeus (Pcm) and bulbospongiosus (Bsm) muscles in female rabbits, it is presently hypothesized that the aromatase expression is differentially linked to the estrogen sensitivity of each muscle. To this end, serum estradiol levels and the aromatase expression, presence of ERα and ERβ and morphometry were evaluated in the Pcm and Bsm of female rabbits allocated in control, ovariectomized (OVX) and OVX treated with estradiol benzoate (OVX + EB) groups. Aromatase expression was high in the Pcm. Independently to serum estradiol, ovariectomy increased aromatase expression in the Pcm while decreased it in the Bsm. The EB treatment avoided the effect of ovariectomy only in the Pcm. The number of immunoreactive nuclei anti-ERα and anti-ERβ was high in the Pcm of OVX and OVX + EB rabbits, while those in the Bsm remained unchanged. The number of peripheral nuclei per fibre and the cross-sectional area-to-myonucleus ratio were modified only in the Pcm. Our findings support aromatase expression in the Pcm, and Bsm of rabbits is differentially linked to estrogenic sensitivity of each muscle.

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“…The present study used anterior vaginal wall tissue since this is an important part of the periurethral support structure that has been examined by others. 20,21 Immunohistochemical staining of vaginal wall fibroblasts in the current study revealed high levels of ERK1/2. Taken together with the reduction in p-ERK1/2 protein in women with SUI compared with controls, this indicates that abnormalities in the ERK1/2 signalling pathway may play a role in the pathogenesis of SUI.…”

Section: Discussionmentioning

confidence: 99%

The role of the ERK1/2 signalling pathway in the pathogenesis of female stress urinary incontinence

et al. 2013

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Objective: To investigate the role of extracellular-regulated protein kinase (ERK)1/2 and phosphorylated (p)-ERK1/2 in the pathogenesis of female stress urinary incontinence (SUI). Methods: Anterior vaginal wall tissue was collected from women with SUI and control subjects. Immunohistochemistry and Western blotting were performed for p-ERK1/2. Primary vaginal fibroblast cultures were incubated in the presence or absence of PD98059 (an inhibitor of ERK kinase) and levels of collagen I and III mRNA and protein were examined by quantitative reverse transcription-polymerase chain reaction and Western blot, respectively. Results: Levels of p-ERK1/2 were significantly lower in vaginal wall tissue from patients with SUI (n ¼ 10) compared with controls (n ¼ 10). PD98059 treatment significantly reduced levels of collagen I and III mRNA and protein.Conclusions: Female SUI is associated with reduced levels of p-ERK1/2 compared with controls, and inhibition of the ERK1/2 signalling pathway inhibits collagen type I and III synthesis in vaginal wall fibroblasts.

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Alterations of estrogen receptor-α and -β in the anterior vaginal wall of women with urinary incontinence

Cited by 29 publications

References 23 publications

Effects of long-term high dose testosterone administration on vaginal epithelium structure and estrogen receptor-α and -β expression of young women

Effects of long-term high dose testosterone administration on vaginal epithelium structure and estrogen receptor-α and -β expression of young women

Aromatase expression is linked to estrogenic sensitivity of periurethral muscles in female rabbits

The role of the ERK1/2 signalling pathway in the pathogenesis of female stress urinary incontinence

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