Alterations of Cell Surface Receptors and Expression of Cellular Oncogenes in the Liver of Rats Fed a Hypolipidemic Peroxisome Proliferator

Hsieh, Ling‐Ling; Weinstein, I. Bernard; Betschart, James M.; Virji, Mohamed A.; Shinozuka, Hisashi

doi:10.1007/978-1-4615-3694-9_8

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“…Relative mRNA abundance was expressed as "fold increase" of specific mRNA by calculating the mean values of the abundance of mRNA in rats fed the CD or BR931 diets to the corresponding value of mRNA in rats fed the CS or basal diets as described in "Materials and Methods." demonstrated that BR931 selectively decreased the number of hepatocyte cell surface receptors with tyrosine b a s e activity, such as insulin and epidermal growth factor receptor (57,58). The receptors for HGF have been shown to be the products of c-met protooncogene with tyrosine b a s e activity (59).…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of Hepatocyte Growth Factor, Transforming Growth Factor–α and Transforming Growth Factor–β1 Messenger Rnas in Two Experimental Models of Liver Cell Proliferation

et al. 1992

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Hepatocyte growth factor, a potent hepatocyte mitogen in vitro, appears to trigger hepatocyte regeneration after partial hepatectomy and after acute liver cell necrosis. Transforming growth factor-alpha and transforming growth factor-beta 1 may also be involved in the control of liver regeneration. In this study we assessed possible roles of hepatocyte growth factor, transforming growth factor-alpha and transforming growth factor-beta 1 on liver cell proliferation in vivo, using a model of choline deficiency that is associated with liver cell necrosis and a model of a hypolipidemic agent (4-chloro-6-(2,3 xylidino)-2-pyrimidinylthio (N-beta-hydroxyethyl) acetamide) without liver necrosis. Male F344 rats were fed a choline-deficient diet or 0.16% 4-chloro-6-(2,3 xylidino)-2-pyrimidinylthio (N-beta-hydroxyethyl) acetamide diet for 6 and 4 wk, respectively. Rats were killed periodically, and the expression of hepatocyte growth factor messenger RNA in the liver, lung and kidney was determined by Northern-blot analysis. The levels of transforming growth factor-alpha and transforming growth factor-beta 1 messenger RNAs in the liver were also determined. Feeding a choline-deficient diet for 1 to 6 wk led to gradual increases in the levels of hepatocyte growth factor, transforming growth factor-alpha and transforming growth factor-beta 1 messenger RNAs in the liver. Feeding a 4-chloro-6-(2,3 xylidino)-2-pyrimidinylthio (N-beta-hydroxyethyl) acetamide diet for 3 days and 2 wk induced marked enhancement of liver cell proliferation as judged by hepatocyte 5-bromo-2-deoxyuridine incorporation.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 99%