Alterations in the relative abundance of gene transcripts in preimplantation bovine embryos cultured in medium supplemented with either serum or PVA

Wrenzycki, C.; Herrmann, Doris; Carnwath, Joseph Wallace; Niemann, Heinrich

doi:10.1002/(sici)1098-2795(199905)53:1<8::aid-mrd2>3.0.co;2-k

Cited by 280 publications

(201 citation statements)

References 76 publications

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“…In our study, hatched blastocysts produced in the defined medium lacking serum produced increased (P \ 0.05) IFNT supporting the notion that mRNA levels for this transcript are higher in good quality embryos. This finding is in agreement with Wrenzycki et al (1999) and Lim et al (2007) who, reported increased levels of IFNT mRNA in blastocysts produced in the presence of PVA compared to when serum was present.…”

Section: Discussionsupporting

confidence: 93%

“…Studies have detected IFNT mRNA in blastocysts but not in morula (Hernandez-Ledezma et al 1992Wrenzycki et al 1999), suggestive that the beginning of IFNT production is closely correlated to blastocoel formation. In our study, hatched blastocysts produced in the defined medium lacking serum produced increased (P \ 0.05) IFNT supporting the notion that mRNA levels for this transcript are higher in good quality embryos.…”

Section: Discussionmentioning

confidence: 99%

“…Although BSA has beneficial effect on embryo development, it is difficult to elucidate specific functions of growth factors or other stimulants as BSA is an undefined mixture of compounds. The presence of serum in a standard culture medium alters the pattern of mRNA expression (Wrenzycki et al 1999;Rizos et al 2003), affects embryo development (Gutierrez-Adan et al 2001) and cryotolerance (Rizos et al 2003), is associated with a reduction in pregnancy rate after transfer to recipients (Lazzari et al 2002), and may produce longterm effects on post-natal development and behaviour (Fernández-Gonzalez et al 2007). Chemically defined conditions without serum or serum derived proteins allow more precise observation of effects of growth or other embryo growth factors in a given medium (Giles and Foote 1997;Sirisathien et al 2003;Oyamada et al 2004).…”

Section: Introductionmentioning

confidence: 99%

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Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

et al. 2016

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The objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. After IVM/IVF presumptive zygotes were cultured in one of three systems: undefined TCM-199, mCR2aa medium supplemented with 10 % FBS and defined PVA-myo-inositol-phosphate-EGF medium. No (P [ 0.05) differences at 2-cell, 4-cell and 8-cell to 16-cell stages were observed among the three cultured media used, however, increased (P \ 0.05) blastocyst yield, cell number and hatching rate were found in defined medium compared to undefined media. The expression patterns of genes implicated in embryo metabolism (GLUT-1), anti-apoptosis (BCL-2), imprinting (IGF-2R), DNA methylation (DNMT-3A) and maternal recognition of pregnancy (IFNT) were increased (P \ 0.05) in hatched blastocysts derived from defined medium compared to undefined media. In conclusion, serum-free, defined medium improved developmental competence of in vitro cultured buffalo embryos. Whether these differences in morphological development and gene expression have long-term effects on buffalo calves born after embryo transfer remains unknown. However, it is possible that early adaptations of the preimplantation embryo to its environment persist during fetal and post-natal development.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

et al. 2016

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“…Approaches in this research area have included studies directly comparing the levels of "marker" gene expression, also variations in embryo metabolism between in vitro and in vivo derived preimplantation embryos and most recently epigenetic re-programming as signified by variation in DNA methylation patterns (Brevini-Gandolfi et al, 1999;De Sousa et al, 1998;Ecker et al, 2004;Knijn et al, 2005;Leese, 2002;Mann et al, 2004;Watson et al, 2000;Wrenzycki et al, 1999;Wrenzycki et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

Bovine oocytes and early embryos express Staufen and ELAVL RNA-binding proteins

Calder

Madan

Watson

2008

Zygote

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ABSTRACT:RNA binding proteins (RBP) influence RNA editing, localization, stability and translation and may contribute to oocyte developmental competence by regulating the stability and turnover of oogenetic mRNAs. The expression of Staufen 1 and 2 and ELAVL1, ELAVL2 RNA binding proteins during cow early development was characterized. Cumulus-oocyte complexes were collected from slaughterhouse ovaries, matured, inseminated and subjected to embryo culture in vitro. Oocyte or preimplantation embryo pools were processed for RT-PCR and whole-mount immunofluorescence analysis of mRNA expression and protein distribution. STAU1 and STAU2, and ELAVL1 mRNAs and proteins were detected throughout cow preimplantation development from the germinal vesicle (GV) oocyte to the blastocyst stage. ELAVL2 mRNAs were detectable from the GV to the morula stage whereas ELAVL2 protein was in all stages examined and localized to both cytoplasm and nuclei. The findings provide a foundation for investigating the role of RBPs during mammalian oocyte maturation and early embryogenesis.

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“…Diverse proteins and glycoproteins could be influencing the extent of early gene expression. There is accumulating evidence that in vitro procedures can alter gene expression in developing embryos [33,34], and they are certainly implicated in perturbations such as the Large Offspring Syndrome. Accordingly, exposure of embryos to Fallopian tube fluid constituents may act to influence reproductive efficiency and normality in diverse mammalian species.…”

Section: Embryonic Gene Expressionmentioning

confidence: 99%

The Fallopian tubes in domestic mammals: how vital is their physiological activity?

Hunter¹

2005

Reprod. Nutr. Dev.

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-Set in an historical perspective, this essay examines diverse physiological aspects of Fallopian tube function in domestic animals and man. Microsurgical experiments are described that established the role of the isthmus in imposing a sperm gradient up to the site of fertilisation. Resection of the isthmus followed by reanastomosis of the remaining portions of the tube generated a high incidence of polyspermy in mated animals. Scanning electron microscopy and surgical studies revealed that spermatozoa were arrested and stored in the caudal portion of the isthmus before ovulation, the so-called functional sperm reservoir. There were specific adhesion contacts between the sperm head and endosalpingeal microvilli or cilia. Further experiments indicated that very large numbers of competent spermatozoa could be released from pre-ovulatory binding by microinjections of a solution of progesterone in oil under the serosal layer of the tube: when suitably timed, such treatment led to a high incidence of polyspermic fertilisation. Avid sperm binding in the caudal isthmus before ovulation prevents myosalpingeal activity leading to abnormal fertilisation, as might occur with multiple mating. Temperatures in the reproductive system were assessed and the caudal isthmus was found to be cooler than the ampulla during the pre-ovulatory phase of sperm storage. Finally, the existence of fluid microenvironments within the Fallopian tubes was reported, and the role of suspended cumulus-corona cells in amplifying signals from the zygote examined. An impact of Fallopian tube fluids on embryonic gene expression was also considered -an influence that may be further imposed if such fluids have access to the uterine lumen. tubal anastomosis / isthmus / sperm gradient / fertilisation / polyspermy / microenvironments / temperature

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Alterations in the relative abundance of gene transcripts in preimplantation bovine embryos cultured in medium supplemented with either serum or PVA

Cited by 280 publications

References 76 publications

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

Bovine oocytes and early embryos express Staufen and ELAVL RNA-binding proteins

The Fallopian tubes in domestic mammals: how vital is their physiological activity?

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