Alterations in the Expression of ATP-Sensitive Potassium Channel Subunit mRNA after Acute Peripheral Nerve and Spinal Cord Injury

Xi, Yin; Fu, Zhong Guo; Zhang, Dian Yin; Jiang, Bailin

doi:10.1159/000097003

Cited by 4 publications

(3 citation statements)

References 63 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, our results showed that there is no change in channel expression for K ATP in the spinal cord under the rat formalin test. This result is different from Yin's report that Kir 6.2 mRNA expression was significantly increased after sciatic nerve injury [23]. This may have resulted from the difference between the inflammatory pain model and the neuropathic pain model in terms of K ATP channel involvement in the pain modulation process.…”

Section: Discussioncontrasting

confidence: 84%

Participation of K_ATP Channels in the Antinociceptive Effect of Pregabalin in Rat Formalin Test

et al. 2011

View full text Add to dashboard Cite

BackgroundPregabalin is an anticonvulsant and analgesic agent that interacts selectively with the voltage-sensitive-Ca2+-channel alpha-2-delta subunit. The aim of this study was to evaluate whether the analgesic action of intrathecal (IT) pregabalin is associated with KATP channels in the rat formalin test.MethodsIT PE-10 catheters were implanted in male Sprague-Dawley rats (250-300 g) under inhalation anesthesia using enflurane. Nociceptive behavior was defined as the number of hind paw flinches during 60 min after formalin injection. Ten min before formalin injection, IT drug treatments were divided into 3 groups: normal saline (NS) 20 µl (CON group); pregabalin 0.3, 1, 3 and 10 µg in NS 10 µl (PGB group); glibenclamide 100 µg in DMSO 5 µl with pregabalin 0.3, 1, 3 and 10 µg in NS 5 µl (GBC group). All the drugs were flushed with NS 10 µl. Immunohistochemistry for the KATP channel was done with a different set of rats divided into naïve, NS and PGB groups.ResultsIT pregabalin dose-dependently decreased the flinching number only in phase 2 of formalin test. The log dose response curve of the GBC group shifted to the right with respect to that of the PGB group. Immunohistochemistry for the KATP channel expression on the spinal cord dorsal horn showed no difference among the groups 1 hr after the formalin test.ConclusionsThe antinociceptive effect of pregabalin in the rat formalin test was associated with the activation of the KATP channel. However, pregabalin did not induce KATP channel expression in the spinal cord dorsal horn.

show abstract

Section: Discussioncontrasting

confidence: 84%

Participation of K_ATP Channels in the Antinociceptive Effect of Pregabalin in Rat Formalin Test

et al. 2011

View full text Add to dashboard Cite

show abstract

“…In another study, both Kir6.1 and SUR2 mRNA were increased in the rats spinal cord 4 and 24 h after the acute spinal cord injury [24]. Our study observed that only Kir6.1 and SUR2 were increased in the cultured neurons after being exposed to Ab 1-42 for 24 h. The possible mechanism may be as follows: first, many studies had demonstrated the Kir6.2 existed in most neurons while the brain mitoK ATP channel may be composed of Kir6.1 [25,26].…”

Section: Discussionmentioning

confidence: 91%

Effects of Aβ1–42 on the Subunits of KATP Expression in Cultured Primary Rat Basal Forebrain Neurons

Zhang

et al. 2008

Neurochem Res

View full text Add to dashboard Cite

ATP-sensitive potassium channels (KATP) play a crucial role in coupling metabolic energy to the membrane potential of cells, thereby functioning as cellular "metabolic sensors." Recent evidence has showed a connection between the amyloid neurotoxic cascade and metabolic impairment. With regard to their neuroprotection in other neuronal preparations, KATP channels may mediate a potential neuroprotective role in Alzheimer's disease (AD). To investigate the effects of Abeta1-42 on the subunits of KATP expression in cultured primary rat basal forebrain cholinergic neurons, primary rat basal forebrain neurons were cultured and evaluated. The subunits of KATP: Kir6.1, Kir6.2, SUR1 and SUR2 expressing changes were observed by double immunofluorescence and immunoblotting when the neurons were exposed to Abeta1-42(2 microM) for different time (0, 24, 72 h). We found a significant increase in the expression of Kir6.1 and SUR2 in the cultured neurons being exposed to Abeta1-42 for 24 h, while Kir6.2 and SUR1 showed no significant change. However, after being treated with Abeta1-42 for 72 h, the expression of the four subunits was all increased significantly compared with the control. These findings suggest that being exposed to Abeta1-42 for different time (24 and 72 h) induces differential regulations of KATP subunits expression in cultured primary rat basal forebrain cholinergic neurons. The change in composition of KATP may contribute to resist the toxicity of Abeta1-42.

show abstract

“…In another study, both Kir6.1 and SUR2 mRNA were increased in the rat spinal cord 4 and 24 h after the acute spinal cord injury [25]. Our study observed that only Kir6.1 and SUR2 were increased in the cultured neurons after being exposed to Ab 1-42 for 24 h. Melamed-Frank reported that hypoxia can up-regulate the expression of Kir6.1 mRNA in vivo and vitro studies, which can change the composition of K ATP channels [26].…”

Section: Discussionmentioning

confidence: 92%

Diazoxide Reverses the Enhanced Expression of KATP Subunits in Cholinergic Neurons Caused by Exposure to Aβ1-42

Gao

et al. 2009

Neurochem Res

View full text Add to dashboard Cite

The ATP-sensitive potassium channel (KATP) play a crucial role in coupling metabolic energy to the cell membrane potential, β-amyloid peptide (Aβ) neurotoxicity has been associated with cellular oxidative stress and metabolic impairment. Whether there is an interaction between KATP and Aβ or not? The expression of KATP subunits was to be investigated after the cultured primary rat basal forebrain cholinergic neurons being exposed to Aβ₁₋₄₂. The subunits of KATP: Kir6.1, Kir6.2, SUR1 and SUR2 expressing change was observed by double Immunofluorescence and immunoblotting in cultured cholinergic neurons from different groups: treatment with Aβ₁₋₄₂ (group Aβ₁₋₄₂), pretreatment with diazoxide and then exposure to Aβ₁₋₄₂ (group diazoxide + Aβ₁₋₄₂), and the control (group control). The results showed that in response to the treatment with Aβ₁₋₄₂ (2 μmol/L) for 24 h, the expression of Kir6.1 and SUR2 were significantly up-regulated, while this change can be partly reversed by pretreatment with diazoxide (1 mmol/L) for 1 h. There were significant increases in all KATP subunits expression levels after exposure to Aβ₁₋₄₂ for 72 h. However, the up-regulation of Kir6.1, Kir6.2 and SUR2 except SUR1 can be partly reversed by pretreatment with diazoxide (1 mmol/L) for 1 h. It is concluded that exposure to Aβ₁₋₄₂ for different time (24 and 72 h) induced differential regulation of KATP subunits expression in cultured primary rat basal forebrain cholinergic neurons. The change in composition of KATP may contribute to the dysfunction of KATP and membrane excitability disturbance. The effect of diazoxide on KATP subunits expression may explain, in part, the resistance of diazoxide to the toxicity of Aβ₁₋₄₂.

show abstract

Alterations in the Expression of ATP-Sensitive Potassium Channel Subunit mRNA after Acute Peripheral Nerve and Spinal Cord Injury

Cited by 4 publications

References 63 publications

Participation of K_ATP Channels in the Antinociceptive Effect of Pregabalin in Rat Formalin Test

Participation of K_ATP Channels in the Antinociceptive Effect of Pregabalin in Rat Formalin Test

Effects of Aβ1–42 on the Subunits of KATP Expression in Cultured Primary Rat Basal Forebrain Neurons

Diazoxide Reverses the Enhanced Expression of KATP Subunits in Cholinergic Neurons Caused by Exposure to Aβ1-42

Contact Info

Product

Resources

About

Alterations in the Expression of ATP-Sensitive Potassium Channel Subunit mRNA after Acute Peripheral Nerve and Spinal Cord Injury

Cited by 4 publications

References 63 publications

Participation of KATP Channels in the Antinociceptive Effect of Pregabalin in Rat Formalin Test

Participation of KATP Channels in the Antinociceptive Effect of Pregabalin in Rat Formalin Test

Effects of Aβ1–42 on the Subunits of KATP Expression in Cultured Primary Rat Basal Forebrain Neurons

Diazoxide Reverses the Enhanced Expression of KATP Subunits in Cholinergic Neurons Caused by Exposure to Aβ1-42

Contact Info

Product

Resources

About

Participation of K_ATP Channels in the Antinociceptive Effect of Pregabalin in Rat Formalin Test

Participation of K_ATP Channels in the Antinociceptive Effect of Pregabalin in Rat Formalin Test