Alterations in T-lymphocyte sub-set profiles and cytokine secretion by PBMC of systemic lupus erythematosus patients upon<i>in vitro</i>exposure to organochlorine pesticides

Dar, Sajad Ahmad; Das, Shukla; Ramachandran, V G; Bhattacharya, S N; Mustafa, Muhammad; Banerjee, Basu Dev; Verma, Prashant

doi:10.3109/1547691x.2011.642103

Cited by 11 publications

(16 citation statements)

References 55 publications

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“…Concordant with those studies, total ENDO isomers have been found in maternal milk (0.6-27.9 ng/ml) (Campoy et al 2001), neonatal blood (9.2 ng/ml) (Schaalan et al 2012) and adult serum (0.2-3 ng/ml) (Wade et al 2002;Dar et al 2012;Pi et al 2016) in the general populations of countries where ENDO is still commonly used in agriculture. With respect to acute exposures, ENDO has been found up to 530 ng/ml in the blood of agricultural workers after application of ENDO in South Africa.…”

Section: Introductionsupporting

confidence: 82%

“…In recent years, there has been an increase in reports of diseases related to impaired immune responses, which may be largely due to environmental contamination by pesticides (Dar et al 2012;Schaalan et al 2012). In Mexico, there is a current problem with the deposition of endosulfan (ENDO), which is a pesticide still at present in use in Mexican agriculture (Wong et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Chronic exposure to endosulfan induces inflammation in murine colon via β-catenin expression and IL-6 production

Téllez‐Bañuelos

Haramati

Franco-Topete

et al. 2016

Journal of Immunotoxicology

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Endosulfan (ENDO) is a widely used organochlorine (OC) pesticide and persistent organo-pollutant. Epidemiological studies have shown that high levels of OC exposure were related to colorectal cancer (CRC) incidence. The objectives of the present study were to evaluate histological changes in the colon, as well as in in situ expression of b-catenin and P-selectin, and serum levels of select pro-inflammatory cytokines in mice administered ENDO; there is a relationship between increased serum IL-6 and P-selectin levels in CRC patients and aberrant b-catenin signaling is important in initiation/maintenance of most CRCs. Mice were exposed to ENDO (at dose < LD 50 ) orally once a week for up to 24 weeks, and monitored (inclusive) for a total of 42 weeks. The experiment was comprised of three groups, one that did not receive ENDO (olive oil vehicle), one administered 2 mg ENDO/kg/week and a positive control (for induction of CRC) given a weekly 20 mg 1,2-dimethylhydrazine (DMH)/kg injection. The results indicated that oral administration of ENDO provoked moderate inflammation starting at six weeks, and severe colonic inflammation with an appearance of dysplastic formations (aberrant crypts) in mice treated with ENDO (or DMH) for 12 weeks or longer. Serum IL-6 levels significantly increased starting at six weeks and rose to a peak of 15-fold higher than in controls at 42 weeks; TNFa levels likewise significantly increased, with a later peak (%four-fold higher than controls) at 30-42 weeks. Immunohistochemical analysis of the colon also showed that expression of b-catenin and P-selectin increased with length of exposure to ENDO. Taken together, the results indicate that continued repeated oral exposure to ENDO induces increased expression of b-catenin and P-selectin, inflammation in the colon, and, ultimately, local tissue dysplasia. ARTICLE HISTORY

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Section: Introductionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Chronic exposure to endosulfan induces inflammation in murine colon via β-catenin expression and IL-6 production

Téllez‐Bañuelos

Haramati

Franco-Topete

et al. 2016

Journal of Immunotoxicology

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“…There was no difference between cases and controls for DDE in a small study conducted the southwestern US (35); however, a number of issues limit inference from this study, most notably inclusion of prevalent (rather than newly diagnosed) cases and the fact that both cases and controls had higher than average pesticide levels compared to the US general population. A study conducted in Delhi, India found significantly higher blood levels of p,p’-DDE, dieldrin, beta-endosulfan, and beta-hexachlorocyclohexane in 13 SLE cases than in 20 healthy controls (36); there was no difference in the other organochlorine pesticides measured. Overall, inferences based on existing epidemiologic data on SLE risk associated with pesticides are limited due to inconsistent results and because no specific pesticide or product has been identified as a likely causal agent.…”

Section: Exposuresmentioning

confidence: 91%

Pesticides, chemical and industrial exposures in relation to systemic lupus erythematosus

Parks

Roos

2014

Lupus

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Growing evidence suggests exposure to chemicals and industrial pollutants may increase risk of SLE. Here we review research on SLE associations with occupational and industrial exposures, primarily drawing on studies in human populations and summarizing epidemiologic research published in the past decade. The association of occupational silica exposure with SLE is well established, but key questions remain, including the required dose and susceptibility factors, and SLE risk due to other silicate exposures. Research on SLE and other exposures is less well developed, though several potential associations merit further consideration due to the consistency of preliminary human findings, experimental animal research, and biologic plausibility. These include pesticides and solvents, for which experimental findings also support investigation of specific agents, including organochlorines and trichloroethylene. Experimental findings and biologic plausibility suggest research on SLE and occupational exposure to hydrocarbons (i.e., mineral oils) is warranted, especially given the widespread exposures in the population. Experimental and limited human findings support further investigation of SLE related to mercury exposure, especially in dental occupations. Research on environmental risk factors in risk-enriched cohorts (family based) is recommended, as is further investigation of exposures in relation to intermediate markers of effect (e.g., antinuclear antibodies), clinical features (e.g., nephritis) and outcomes.

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“…At lower concentrations, the response may be very different according to the specific mitogen used and exposure duration. In this respect, other works have shown that cell proliferation can be increased by treatment with pesticides at µM level (Balakrishnan et al, 2002;Giribaldi et al, 2011) while long or repeated exposure to OCPs is often associated with decreased response to mitogens (Vine et al, 2001;Dar et al, 2012). In addition, the differences in responsiveness of PBMCs under mitogen stimulation may be explained in the light of the differential impact of -HCH on different lymphocyte sub-populations (Neri et al, 1973;Mellstedt, 1975).…”

Section: Pwmmentioning

confidence: 92%

“…The concentration range was defined on the basis of an extensive literature review regarding the toxicological and bio-medical sector; it has evidenced that, for this compound, the concentrations in the order of µM were usually tested in vitro (Steinmetz et al, 1996;Payne et al, 2001;Zou and Matsumura, 2003;Aubé et al, 2011). However, in some studies on human PBMCs, also concentrations up to the order of mM have been used (Ennaceur et al, 2008;Dar et al, 2012). The final concentration of DMSO was always 0.2% (v/v) as chosen on the basis of preliminary experiments to test the toxicity of DMSO.…”

Section: Cells and Chemicalsmentioning

confidence: 99%

In VitroEffects of the Organochlorine Pesticide β-Hexachlorocyclohexane on Bovine Peripheral Blood Mononuclear Cells

Rossi

Danieli

Ronchi

2014

Italian Journal of Animal Science

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The -hexachlorocyclohexane ( -HCH) is a very stable and accumulable isomer of Lindane, a well known organochlorine pesticide. The HCHs were banned in all developed countries but to date high concern still exists for environment, animal and human health due to contaminated sites. In this study, several in vitro tests [cell viability (XTT), trypan blue exclusion (TBE), lactate dehydrogenase release (LDH) and bromodeoxyuridine (BrdU) incorporation assays] were performed to investigate the toxic effects of exposure to -HCH (from 0.1 to 1000 µM) on bovine peripheral blood mononuclear cells (PBMCs). All the trials were performed incubating PBMCs for 2 and 7 days. At high concentrations (i.e. 1000 µM), the -HCH approximately halved the number of living cells regardless the exposure time, significantly decreased the cell viability assessed by the XTT assay, and compromised the proliferation potential of PBMCs. At lower -HCH exposure levels (0.1 to 100 µM), particularly after 7 days of exposure, a progressive decrease of cell viability has been observed. These adverse effects were significant at concentrations observed in the blood of cattle reared in polluted areas. The LDH results suggest that -HCH does not clearly affect the integrity of the cell membrane in the range of exposure levels tested. All in all, these findings warn about the risk posed by the long-term exposure to -HCH of farm animals reared in rural areas polluted by -HCH. Further research is needed to deepen our knowledge about the mechanisms through which -HCH affects the PBMCs functionality.

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Alterations in T-lymphocyte sub-set profiles and cytokine secretion by PBMC of systemic lupus erythematosus patients uponin vitroexposure to organochlorine pesticides

Abstract: (2012) Alterations in Tlymphocyte sub-set profiles and cytokine secretion by PBMC of systemic lupus erythematosus patients upon invitro exposure to organochlorine pesticides, Journal of Immunotoxicology, 9:1, 85-95,

Cited by 11 publications

References 55 publications

Chronic exposure to endosulfan induces inflammation in murine colon via β-catenin expression and IL-6 production

Chronic exposure to endosulfan induces inflammation in murine colon via β-catenin expression and IL-6 production

Pesticides, chemical and industrial exposures in relation to systemic lupus erythematosus

In VitroEffects of the Organochlorine Pesticide β-Hexachlorocyclohexane on Bovine Peripheral Blood Mononuclear Cells

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