Alterations in some blood coagulation parameters in naturally occurring cases of canine babesiosis

Rafaj, Renata Barić; Matijatko, Vesna; Kiš, Ivana; Kučer, Nada; Živičnjak, Tatjana; Lemo, Nikša; Žvorc, Zdravko; Brkljačić, Mirna; Mrljak, Vladimir

doi:10.1556/avet.57.2009.2.10

Cited by 27 publications

(15 citation statements)

References 32 publications

(46 reference statements)

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“…Previous studies on B. canis have shown that marked thrombocytopenia was the most common hemostatic change, however very few cases show any gross clinical signs of primary hemostatic abnormalities (Rafaj et al, 2005;Rafaj et al, 2009;Ruiz et al, 2007;Schetters et al, 2009). A similar severe thrombocytopenia is seen in B. rossi with platelet counts reported as low as 14×10 9 /L being a common finding without any associated clinical signs of hemorrhage (Kettner et al, 2003).…”

Section: Discussionmentioning

confidence: 84%

Mortality in virulent canine babesiosis is associated with a consumptive coagulopathy

Goddard¹,

Wiinberg²,

Schoeman³

et al. 2013

The Veterinary Journal

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The inflammatory response to infection can activate the coagulation system via complex interactions. If uncontrolled, this may lead to a consumptive coagulopathy, which has been identified as a major risk factor for poor outcome in both human and canine medicine. This study was undertaken to prospectively determine whether the presence of a consumptive coagulopathy in dogs with Babesia rossi infection is related to mortality. A prospective, cross-sectional, observational study was performed. Seventy-two client-owned dogs diagnosed with canine babesiosis were included. Diagnosis was confirmed by polymerase chain reaction and reverse line blot and dogs infected with Babesia vogeli or Ehrlichia canis were excluded. Blood samples were collected at admission. Coagulation factor-, antithrombin (AT)-, and protein C (PC) activity, prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen and Ddimer concentrations were measured. The mortality rate was 18% (13/72) and results between non-survivors and survivors were compared. The median activities of all the coagulation factors were significantly lower in the non-survivors compared to the survivors. The median PT and aPTT were significantly longer in the non-survivors compared to the survivors. The median AT activity was not significantly different; however, the median PC activity was significantly 2 decreased in the non-survivors. The median D-dimer concentration was significantly higher in the non-survivors compared to the survivors. This study showed that dogs that died from B.canis infection suffered from a more severe consumptive coagulopathy compared to survivors, characterized by procoagulant activation, inhibitor consumption, and increased fibrinolytic activity.

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Section: Discussionmentioning

confidence: 84%

Mortality in virulent canine babesiosis is associated with a consumptive coagulopathy

Goddard¹,

Wiinberg²,

Schoeman³

et al. 2013

The Veterinary Journal

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“…It is a circulating glycoprotein that can inhibit thrombin directly and via a number of upstream factors. The AT III levels were significantly decreased in dogs with babesiosis (spot 432) indicating an imbalance in haemostasis defined by the overproduction of thrombin and fibrin [41]. This could be a consequence of inhibitor breakdown and/or liver dysfunction affecting inhibitor production in dogs with babesiosis.…”

Section: Discussionmentioning

confidence: 99%

Identification of serum biomarkers in dogs naturally infected with Babesia canis canis using a proteomic approach

et al. 2014

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BackgroundCanine babesiosis is a tick-borne disease that is caused by the haemoprotozoan parasites of the genus Babesia. There are limited data on serum proteomics in dogs, and none of the effect of babesiosis on the serum proteome. The aim of this study was to identify the potential serum biomarkers of babesiosis using proteomic techniques in order to increase our understanding about disease pathogenesis.ResultsSerum samples were collected from 25 dogs of various breeds and sex with naturally occurring babesiosis caused by B. canis canis. Blood was collected on the day of admission (day 0), and subsequently on the 1st and 6th day of treatment.Two-dimensional electrophoresis (2DE) of pooled serum samples of dogs with naturally occurring babesiosis (day 0, day 1 and day 6) and healthy dogs were run in triplicate. 2DE image analysis showed 64 differentially expressed spots with p ≤ 0.05 and 49 spots with fold change ≥2. Six selected spots were excised manually and subjected to trypsin digest prior to identification by electrospray ionisation mass spectrometry on an Amazon ion trap tandem mass spectrometry (MS/MS). Mass spectrometry data was processed using Data Analysis software and the automated Matrix Science Mascot Daemon server. Protein identifications were assigned using the Mascot search engine to interrogate protein sequences in the NCBI Genbank database.A number of differentially expressed serum proteins involved in inflammation mediated acute phase response, complement and coagulation cascades, apolipoproteins and vitamin D metabolism pathway were identified in dogs with babesiosis.ConclusionsOur findings confirmed two dominant pathogenic mechanisms of babesiosis, haemolysis and acute phase response. These results may provide possible serum biomarker candidates for clinical monitoring of babesiosis and this study could serve as the basis for further proteomic investigations in canine babesiosis.

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“…Canine babesiosis was considered the most probable diagnosis, as it can also cause secondary haemostatic disorders (Rafaj et al . , Goddard et al . ).…”

Section: Resultsmentioning

confidence: 99%

Implication, clinical and biological impact of vector‐borne haemopathogens in anaemic dogs in France: a prospective study

Bouzouraa

Cadoré

Chêne

et al. 2017

J of Small Animal Practice

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Objectives To prospectively describe the clinical and biological impact of vector‐borne haemopathogens in anaemic dogs in France and occurrence of haemolysis. Materials and Methods This prospective descriptive cohort study includes 134 client‐owned dogs that were anaemic on admission at the Veterinary Teaching Hospital of VetAgro Sup, Lyon, France. They underwent comprehensive screening with PCR to detect a panel of vector‐borne haemopathogens, SNAP Leishmania and SNAP 4Dx Plus (IDEXX). Results Vector‐borne haemopathogen‐associated anaemia accounted for 17·2% (23/134) of anaemic cases. PCR for Babesia species, Mycoplasma species, Anaplasma platys or Ehrlichia canis and positive serology for Leishmania species, occurred in 11/23, 10/23, 2/23, 1/23 and 1/23 cases, respectively. Two dogs had positive PCR for both Babesia and Mycoplasma species. All dogs infected with Mycoplasma species alone had neoplastic disease. Vector‐borne haemopathogens were the second most common cause of haemolysis (7/23, 30·4%). In the multivariable regression model, males were overrepresented ( OR : 2·82, P=0·03) and haemolysis ( OR : 3·31, P=0·01) was more frequent in dogs with vector‐born haemopathogen‐associated anaemia. Clinical Significance Vector‐borne haemopathogens are a common cause of anaemia in this geographical region.

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Alterations in some blood coagulation parameters in naturally occurring cases of canine babesiosis

Cited by 27 publications

References 32 publications

Mortality in virulent canine babesiosis is associated with a consumptive coagulopathy

Mortality in virulent canine babesiosis is associated with a consumptive coagulopathy

Identification of serum biomarkers in dogs naturally infected with Babesia canis canis using a proteomic approach

Implication, clinical and biological impact of vector‐borne haemopathogens in anaemic dogs in France: a prospective study

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