Alterations in serum electrolytes, antioxidative enzymes and haematological parameters of Labeo rohita on short-term exposure to sublethal dose of nitrite

Ciji, Alexander; Sahu, Narottam Prasad; Pal, A. K.; Dasgupta, Subrata; Akhtar, M. S.

doi:10.1007/s10695-012-9623-3

Cited by 63 publications

(30 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A reduction in WBC count has been reported in Indian major carps upon nitrite exposure (Das et al . ; Ciji, Sahu, Pal, Dasgupta & Akhtar ). Dietary supplementation of higher levels of tryptophan might have helped in counteracting the nitrite induced stress as evidenced by increase in WBC count in these groups.…”

Section: Discussionmentioning

confidence: 99%

Dietary L-tryptophan modulates growth and immuno-metabolic status ofLabeo rohitajuveniles exposed to nitrite

et al. 2013

Self Cite

View full text Add to dashboard Cite

Effect of nitrite exposure on growth and immunometabolic responses of Labeo rohita fed L-tryptophan (TRP) was studied. Fish previously fed normal and elevated levels of tryptophan for 60 days were exposed to nitrite (2.0 mg L À1 ) for another 45 days with same feeding regime. There were four treatment groups, viz., TRP 0 -N (control), TRP 0 +N, TRP 0.75 +N (0.75% supplemental tryptophan in the diet) and TRP 1.5 +N (1.5% supplemental tryptophan in the diet). Highest weight gain% and SGR were observed in control and lowest in TRP 0 +N. Dietary supplementation of elevated levels of tryptophan augmented weight gain% and SGR. Nitrite exposed groups recorded higher catalase, SOD, LDH, AST and ALT activities compared with control. However, activities reduced with additional levels of tryptophan supplementation. Nitrite exposure reduced WBC count, total protein, albumin, globulin and lysozyme activity compared with unexposed group but groups which were fed additional amounts of tryptophan restored total protein, albumin and globulin similar to TRP 0 -N. In conclusion, nitrite exposure had adversely affected growth, increased activities of LDH, AST, ALT, catalase, but decreased WBC, serum protein, lysozyme and acetylcholine esterase activity of L. rohita. Normal requirement of tryptophan was unable to combat nitrite stress. However, dietary fortification with tryptophan (minimum 0.75% of diet + normal requirement) found effective in combating nitrite induced stress.

show abstract

Section: Discussionmentioning

confidence: 99%

Dietary L-tryptophan modulates growth and immuno-metabolic status ofLabeo rohitajuveniles exposed to nitrite

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Another potential explanation for the positive effects of tryptophan in fish exposed to toxic substances might be mitigation of oxidative stress through its antioxidant property, as described above. This is because the toxic agents used in the commented studies are all known to cause oxidative damage in fish (Rom eo et al 2000;Sanchez et al 2005;Vutukuru et al 2006;Ciji et al 2012Ciji et al , 2015Jia et al 2015;Wei & Yang 2016).…”

Section: Tryptophan Antitoxic Effectsmentioning

confidence: 99%

“…Being the precursor of 5HT and melatonin, tryptophan modulates behaviour, mood and stress responses in fish (Winberg & Lepage 1998;Winberg et al 2001;Lepage et al 2002Lepage et al , 2005aHerrero et al 2007;Azpeleta et al 2010). Overall, the different tryptophan metabolites have a wide range of physiological roles including modulation of stress response, antioxidant system, immune response and behavioural responses (H€ oglund et al 2005;Tejpal et al 2009;Hoseini & Hosseini 2010;Ciji et al 2012Ciji et al , 2013bCiji et al , 2015Hoseini et al 2012Hoseini et al , 2016Wolkers et al 2012;Akhtar et al 2013a,b;Hosseini & Hoseini 2013;Azeredo et al 2016;Cort es et al 2016). The knowledge regarding tryptophan effects on teleosts is currently scarce compared to that from mammals.…”

Section: Introductionmentioning

confidence: 99%

Physiological roles of tryptophan in teleosts: current knowledge and perspectives for future studies

Hoseini

Pérez‐Jiménez

Costas

et al. 2017

Reviews in Aquaculture

View full text Add to dashboard Cite

Tryptophan is an essential amino acid with a huge functional versatility, in addition to its participation in protein synthesis. Because of the complexity of its metabolism, and the functional relevance of several of its metabolites, it directly or indirectly participates in a wide array of physiological pathways. This amino acid is a precursor for the synthesis of the neurotransmitter/neuromodulator serotonin (5HT), the hormone melatonin and kynurenine and related compounds such as kynurenic acid, quinolinic acid or niacin. Because of this, it has a key role in the regulation of processes ranging from the neuroendocrine to the immune system in vertebrates. In aquaculture, extensive research has been performed to optimize the levels of tryptophan in the commercial diets for many fish species. Providing adequate levels of this amino acid is critically important for fish growth but also for fish welfare, as tryptophan has been shown to modulate fish behaviour, stress responses, and antioxidant and immune systems. Currently, available data suggest a wide variation in tryptophan requirements of different species ranging 0.3–1.3% of dietary protein level, but recent evidence also shows that fish tryptophan requirements can greatly vary depending on the rearing conditions of the fish. We also review here the participation of tryptophan and related metabolites in different physiological functions that are crucial for fish welfare. The review covers the involvement of tryptophan in 5HT‐ and melatonin‐mediated functions, along with its participation in the regulation of the immune system and its role as an antioxidant and antitoxic agent in fish.

show abstract

“…The transport mechanism involved in the K + losses from the muscle seems to be similar to that observed in RBCs (Knudsen and Jensen ). Hyperkalemia has been frequently demonstrated in fish as a consequence of nitrite intoxication (Grosell and Jensen ; Gisbert et al ; Ciji et al ; Jia et al ). Nonetheless, the recovery period was sufficient to restore the basal levels of blood K + , which was the only altered ionic parameter at the end of the nitrite exposure.…”

Section: Discussionmentioning

confidence: 99%

Secondary Stress Responses in Juvenile Brazilian Flounder, Paralichthys orbignyanus, throughout and after Exposure to Sublethal Levels of Ammonia and Nitrite

Maltez

Barbas

Okamoto

et al. 2018

J World Aquaculture Soc

View full text Add to dashboard Cite

This study investigated the secondary stress responses of Paralichthys orbignyanus exposed to ammonia and nitrite and after recovery. Fish were exposed to 0.12, 0.28, and 0.57 mg NH3‐N/L, or 5.72, 10.43, and 15.27 mg NO2‐N/L for 10 d followed by the same time length for recovery. Ammonia‐ and nitrite‐free water was used as a control treatment. Blood samples were collected after 1, 5, and 10 d of exposure and after recovery. Fish exposed to ammonia presented lower and higher glucose levels after 10 d of exposure and recovery, respectively. Ammonia induced initial and transient ionic disturbances and metabolic alkalosis. Nitrite exposure caused hyperglycemia, increased plasma K+ levels, and respiratory alkalosis, whereas metabolic acidosis was observed after recovery. Increased proportion of monocytes and/or granulocytes and reduced number of lymphocytes were demonstrated in fish exposed to 0.28 mg NH3‐N/L (Day 1) and 10.43 mg NO2‐N/L (Day 5) and after recovery in the 0.28 and 0.57 mg NH3‐N/L treatments. Exposure to ammonia decreased the proportion of granulocytes on Day 5. In conclusion, exposure to concentrations at 0.12 mg NH3‐N/L and 5.72 mg NO2‐N/L provoked physiological disorders in Brazilian flounder. Nonetheless, fish exposed to 5.72 mg NO2‐N/L following a 10‐d recovery period showed complete resumption of homeostasis.

show abstract

Alterations in serum electrolytes, antioxidative enzymes and haematological parameters of Labeo rohita on short-term exposure to sublethal dose of nitrite

Cited by 63 publications

References 45 publications

Dietary L-tryptophan modulates growth and immuno-metabolic status ofLabeo rohitajuveniles exposed to nitrite

Dietary L-tryptophan modulates growth and immuno-metabolic status ofLabeo rohitajuveniles exposed to nitrite

Physiological roles of tryptophan in teleosts: current knowledge and perspectives for future studies

Secondary Stress Responses in Juvenile Brazilian Flounder, Paralichthys orbignyanus, throughout and after Exposure to Sublethal Levels of Ammonia and Nitrite

Contact Info

Product

Resources

About