Alterations in Oxidative Stress-Related Parameters in Rat Testis Following Monensin Administration

Abstract

Monensin, a carboxylic ionophore, is well known for Na(+)/H(+) exchanger activity across biological membranes. It is also used in the poultry industry for its useful effects as a food additive. The present study has been designed to investigate the effects of monensin on some oxidative stress-related parameters in rat testis. Monensin was administered intratesticularly (5 mug/testis) to both testes by a single dose to Wistar rats for different time periods. After the completion of the respective treatments, various parameters reflecting the antioxidant defense system of the tissue were monitored and marked changes were found in the activities of various enzymes as well as in the levels of reduced glutathione and lipid peroxidation. After 1, 2, 3, and 4 days of monensin treatment, the activity of superoxide dismutase was found to be unaltered. However, after 2 days of monensin treatment, glutathione-S-transferase and catalase showed inhibition in their activities along with the depletion of glutathione (reduced) accompanied by a marked increase in lipid peroxidation. The increase in lipid peroxidation was noticeable even after 1 day of monensin administration. The inhibition in glutathione-S-transferase and glutathione peroxidase activities was also observed along with an increase in lipid peroxidation at the end of the 3-day posttreatment period, while, the 4-day posttreatment schedule caused an increase in the activity of glutathione reductase and glutathione peroxidase that was also accompanied by an inhibition of catalase. The findings of the present study are indicative of the potential of monensin in testicular tissue in contraceptive intervention.

Effects of Lasalocid or Monensin Supplementation on Digestion, Ruminal Fermentation, Blood Metabolites, and Milk Production of Lactating Dairy Cows

“…Least squares means with SEM given for n = 5. tratesticular administration of MON (5 g/testis) to rats induced a marked increase in lipid peroxidation (Singh et al, 2006). Supplementation with IOP also induced a metabolic alkalosis, as shown by the greater (P = 0.01) concentration of serum bicarbonate associated with a lower (P = 0.03) anion gap value.…”