Alterations in oligodendrocyte proteins, calcium homeostasis and new potential markers in schizophrenia anterior temporal lobe are revealed by shotgun proteome analysis

Abstract

Global proteomic analysis of post-mortem anterior temporal lobe samples from schizophrenia patients and non-schizophrenia individuals was performed using stable isotope labeling and shotgun proteomics. Our analysis resulted in the identification of 479 proteins, 37 of which showed statistically significant differential expression. Pathways affected by differential protein expression include transport, signal transduction, energy pathways, cell growth and maintenance and protein metabolism. The collection of protein alterations identified here reinforces the importance of myelin/oligodendrocyte and calcium homeostasis in schizophrenia, and reveals a number of new potential markers that may contribute to the understanding of the pathogenesis of this complex disease.

Decreased calcium pump expression in human erythrocytes is connected to a minor haplotype in the ATP2B4 gene

“…Several recent studies found potential associations between SNPs in the ATP2B4 gene, and diseases or disease-related conditions, including reduced bone mass [21], increased incidence of schizophrenia [22], cardiac hypertrophy [23], red blood cell traits [24], and the incidence of malaria infection [25]. In GWAS studies the SNP rs7551442 in the ATP2B4 gene was specifically linked to red blood cell MCHC (mean corpuscular hemoglobin concentration, Ref.…”

Thalamic transcriptome screening in three psychiatric states

“…38 Of our MDNp qPCR-validated genes (Table 2), YWHAH and YWHAZ, have been previously shown to be differentially expressed in the temporal lobe in SZ. 39 Of the qPCR-validated genes on the pHcorrected analysis (Table 2 marked with the superscript letter 'a'), DNAJB6 (DnaJ (Hsp40) homolog, subfamily B, member 6 (also known as heat shock protein J2), RAD1 (RAD1 homolog (Schizosaccharomyses pombe) (aka checkpoint control protein HRAD1) and NRXN3 (neurexin 3) will be discussed with regard to SZ.…”

Common Proteomic Changes in the Hippocampus in Schizophrenia and Bipolar Disorder and Particular Evidence for Involvement of Cornu Ammonis Regions 2 and 3

“…37 Usingproteomicmethods, it is possible to assess global differential protein expression between disease and control states and to obtain novel insights into disease (for reviews, see the articles by English etal, 38 Görgetal, 39 andTannuandHemby 40 ).Proteomicstudies of postmortem brain tissue from subjects with schizophrenia and from subjects with bipolar disorder have focused largely on the dorsolateral prefrontal cortex [41][42][43][44][45][46][47][48][49] and the anterior cingulate cortex. [50][51][52][53] They have demonstrated alterations in cytoskeletal, synaptic, metabolic, and mitochondrial proteins. 42,[44][45][46]48,50,54 To our knowledge, with regard to the hippocampus, there has been 1 previous proteomic study in schizophrenia 54 and none in bipolar disorder.…”