Alterations in neurogenically mediated contractile responses of urinary bladder in rats with diabetes

Liu, Guiming; Daneshgari, Firouz

doi:10.1152/ajprenal.00449.2004

Cited by 87 publications

(89 citation statements)

References 54 publications

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“…Autonomic and sensory neuropathy is suspected in the pathogenesis of several diseases of the bladder including the overactive bladder and diabetic cystopathy 1,2 . Despite strong evidence indicating the presence of increased or altered afferent autonomic sensitivity in these pathologies, to date, there exists no reliable way to measure the bladder manifestations of these conditions.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of a Novel Device for Assessment of Autonomic Sensory Function in the Rat Bladder

et al. 2008

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Purpose-We developed and tested the efficacy of an implantable bladder device which, when combined with the Neurometer®, can be used to assess fiber-specific afferent sensation of the bladder in the rat.Materials and Methods-We developed an implantable bladder device which applies selective nerve fiber stimuli (250 Hz for small myelinated (Aδ), and 5 Hz for unmyelinated (C) fibers) to the bladder mucosa in the rat in order to obtain the bladder sensory perception threshold (SPT) values. We performed three experiments on fifty-five female Sprague-Dawley rats, examining the effects of our device on voiding habits; assessing the inter-observer reliability of SPT; and the effects of intravesical administration of resiniferatoxin and lidocaine on the SPT. Results-The SPT values obtained by two blinded, independent observers were not different from one another (p= 0.41). The SPT values obtained at both stimulation frequencies remained constant for at least 3 weeks after device implantation. A significant increase in SPT values after instillation of resiniferatoxin (p = 0.02) was noted at a stimulus frequency of 5 Hz, whereas intravesical lidocaine led to an immediate increase in SPT at both 250 and 5 Hz. Device implantation led to an early decreased voided volume and increased frequency of voids, however these parameters returned to normal after 4 days.Conclusions-Assessment of bladder afferent sensation with our newly developed device is feasible in rats, and provides sensory perception thresholds that appear to be fiber-type selective for autonomic bladder afferent nerves.

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Section: Introductionmentioning

confidence: 99%

Efficacy of a Novel Device for Assessment of Autonomic Sensory Function in the Rat Bladder

et al. 2008

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“…These results are in agreement with other studies utilizing the same tissue and stimulating technique. 4,5,19 EFS-induced contractions are mediated by muscarinic receptors, namely M 2 and M 3 receptors although M 1 , M 4 and M 5 muscarinic receptors are also present in the rat detrusor muscle. 20,21 The signalling mechanisms for muscarinc receptors vary amongst the different subtypes and species and have yet to be completely understood.…”

Section: Fig6: Represents Images Of Haematoxylin and Eosin Stained Smentioning

confidence: 99%

“…6,12 No clear reason for the discrepancies in published results has been ascertained, however, it is thought a poor or a complete lack of standardization in the contractile force generated may be to blame. 5 In addition, discrepancies may arise due to inadequate experimental protocols i.e. usage of different regions of the urinary bladder tissue between both groups, or the length and severity of the disease.…”

Section: Fig6: Represents Images Of Haematoxylin and Eosin Stained Smentioning

confidence: 99%

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Sangar¹,

Shakil²,

Singh³

2016

IJPSR

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ABSTRACT:Patients with DM are often afflicted by debilitating urologic complications. The main aim of this study is to investigate the effect of streptozotocin (STZ)-induced type I diabetes on contractile responses to numerous stimuli, intracellular cation concentrations and morphology in the rat. Adult rats (n=12) were humanely killed and detrusor muscles of urinary bladder located and excised rapidly and placed in organ baths. Then urinary bladder detrusor muscles were blotted, weighed and dissolved in concentrated nitric acid for the measurements of cation contents. The diabetic rats had significantly (P<0.05) elevated blood glucose compared to control rats. The contractile responses induced by EFS were significantly (P<0.05) increased in the diabetic urinary bladder tissue in comparison to the control urinary bladder tissue. The increase in the contractile force was calcium-dependent in both control and diabetic urinary bladder tissues. The results also showed no significant differences in the levels of sodium, potassium, calcium, between the diabetic and control urinary bladder (p>0.05). However, there was a significant (P<0.05) decrease in magnesium in the diabetic urinary bladder tissue in comparison to the control urinary bladder tissue. The increased responsiveness of the diabetic urinary bladder tissue compared to the control tissue may be due to either alterations in the intracellular cation concentrations, hypertrophy of the diabetic urinary bladder or calcium sensitivity to the myofilaments. The results also raise the possibility that that these factors, in conjunction with other causes may work in tandem to exacerbate diabetic bladder dysfunction.

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“…We have studied bladder dysfunction in type 1 diabetic rodents systematically, investigating alterations in morphometry (9), functionality (10,11), contractility (11,12), and nerves and vasculature (12,13) of the bladder in response to streptozotocin (STZ)-induced type 1 diabetes compared with osmotically induced polyuria. We have revealed temporal effects of diabetes, causing an early phase of compensatory bladder function and a later phase of decompensated bladder function.…”

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confidence: 99%

Tissue Specific Dysregulated Protein Subnetworks in Type 2 Diabetic Bladder Urothelium and Detrusor Muscle

Tomechko¹,

Liu

Tao

et al. 2015

Molecular & Cellular Proteomics

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Diabetes mellitus is well known to cause bladder dysfunction; however, the molecular mechanisms governing this process and the effects on individual tissue elements within the bladder are poorly understood, particularly in type 2 diabetes. A shotgun proteomics approach was applied to identify proteins differentially expressed between type 2 diabetic (TallyHo) and control (SWR/J) mice in the bladder smooth muscle and urothelium, separately. We were able to identify 1760 nonredundant proteins from the detrusor smooth muscle and 3169 nonredundant proteins from urothelium. Pathway and network analysis of significantly dysregulated proteins was conducted to investigate the molecular processes associated with diabetes. This pinpointed ERK1/2 signaling as a key regulatory node in the diabetes-induced pathophysiology for both tissue types. Diabetes mellitus is reaching epidemic proportions worldwide because of several factors, most notably obesity, which is attributed to increased sedentary lifestyles and decreased physical activity. This presents both economic and healthcare challenges; the U.S., China, and India have the highest disease prevalences and therefore are facing the greatest challenges (1). According to the Centers for Disease Control and Prevention, as of 2010, 25.8 million Americans have diabetes, and it is the seventh leading cause of death in the United States. Approximately 30% of U.S. adults 20 years or older and 50% of adults 75 years or older are afflicted with this debilitating disease (2). Based on current trends, it is expected that the number of patients diagnosed with diabetes will continue to climb at an alarming rate.Some complications of diabetes include heart disease and stroke, hypertension, blindness and eye problems, kidney disease, and nervous system disease. Recently, diabetic urological complications such as nephropathy, bladder dysfunction (3) and infection, incontinence, erectile dysfunction (4), and prostate hyperplasia have been receiving increasing attention, because they affect both type 1 and type 2 diabetic patients. Diabetic uropathy, which includes diabetic bladder dysfunction (DBD) 1 , sexual or erectile dysfunction, and urinary tract infection (5), has been found in more than 80% of patients with diabetes, a higher rate of incidence than either neuropathy or nephropathy, which affect 60 and 50% of From the ‡Center

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Alterations in neurogenically mediated contractile responses of urinary bladder in rats with diabetes

Cited by 87 publications

References 54 publications

Efficacy of a Novel Device for Assessment of Autonomic Sensory Function in the Rat Bladder

Efficacy of a Novel Device for Assessment of Autonomic Sensory Function in the Rat Bladder

Untitled

Tissue Specific Dysregulated Protein Subnetworks in Type 2 Diabetic Bladder Urothelium and Detrusor Muscle

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