Alterations in Membrane Protein-Profile during Cold Treatment of Alfalfa

Mohapatra, Shyam S.; Poole, Ronald J.; Dhindsa, Rajinder S.

doi:10.1104/pp.86.4.1005

Cited by 16 publications

(6 citation statements)

References 10 publications

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“…During hardening a number of specific proteins accumulate to protect the cytoplasm and membranes from disruption from the formation of ice crystals within the cells on freezing (KacperskaPalacz et al 1977). In addition to changes in the soluble polypeptide complement, reports have been made of alterations in the membrane polypeptide fraction (Mohapatra et al 1988;Robertson et at. 1988).…”

Section: Resultsmentioning

confidence: 96%

Acclimation to freezing temperatures in perennial ryegrass (Lolium perenne)

et al. 1997

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The increasing demands being placed on natural grasslands in the era following the appearance of Bovine Spongiform Encephalitis require that forage crops provide a reliable extended season of growth, combined with good winter survival to ensure sward longevity. The ability to tolerate sub-zero temperatures is integral to the survival of perennial forages. Since the development of freezing tolerance is crucial to the survival and productivity of over-wintering crops, forage breeding programmes require an improved understanding of the individual characteristics that contribute to tolerance to sub-zero temperatures. Photosynthesis, carbohydrate content and changes in protein composition were investigated in two varieties of Lolium perenne which differ in their response to growth at low temperature.

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Section: Resultsmentioning

confidence: 96%

Acclimation to freezing temperatures in perennial ryegrass (Lolium perenne)

et al. 1997

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“…While studies on senescence as a programmed event have revolved around gene expression for carbohydratedegrading enzymes (polygalacturonase, cellulase and invertase), it is reasonable to believe that others (including oxidative enzymes) are also under genomic regulation. With respect to chilling stress, exposure of tomato plants to low temperature leads to the rapid accumulation of specific mRNAs (Schaffer and Fischer 1988) and cold-acclimation of alfalfa induces the synthesis of several new membrane-associated polypeptides (Mohapatra et al 1988).…”

Section: Does Chilling Induce Senescent Breakdown In Cs Plants?mentioning

confidence: 99%

Chilling Injury. A Review of Possible Mechanisms

Parkin

Marangoni

Jackman

et al. 1989

J Food Biochemistry

143

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Chilling injury (CI) is a physiological defect of plants and their products that results in reduced quality and loss of product utilization following exposure to low but nonfreezing temperatures. To design more effective control strategies and maximize shelf‐life, it is necessary to develop an understanding of the biochemical mechanism(s) responsible for the initiation of CI. Despite considerable efforts in this field of study, there is no general agreement on the cause or nature of CI, or even the primary event(s) triggering low temperature damage. The first unified theory to explain CI was founded on low temperature induced membrane lipid phase transitions leading to a loss of membrane integrity and physiological dysfunction. This was modified to account for the observation that the level of certain high melting phospholipids appears to be related to the chill sensitivity of many plant tissues. Membranes and changes in their physical characteristics are further implicated as having a role in CI by the discovery that chilling stress evokes an elaborate membrane retailoring response that leads to increased fluidity at reduced temperatures. Others have postulated that CI results from the direct effect of reduced temperatures on enzymes or the indirect effect of membrane perturbations on intrinsic enzymes. The redistribution of cellular calcium has most recently been advanced as the primary transducer of CI. The weight of this theory rests on the role of calcium as a secondary messenger for many cellular functions. In this review it is also speculated that lipid peroxidation may have a role in the development of irreversible injury during low temperature stress. Its effect would be similar to the senescent processes of free radical damage to tissue and progressive membrane rigidification.

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“…Since membrane stabilization is an important factor in plant freezing tolerance, and some low-temperature proteins have been found to be associated with membrane fractions (Mohapatra et al, 1988), we wished to determine whether BN28 fractionated with cellular membranes. This was accomplished by separating leaf extracts into soluble and microsomal pellet fractions.…”

Section: Immunological Characterizationmentioning

confidence: 99%

Purification, Characterization, and Structural Analysis of a Plant Low-Temperature-Induced Protein

et al. 1997

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We have purified to near homogeneity a recombinant form of the protein BN28 (rBN28), expressed in response to low temperature in Brassica napus plants, and we have determined its solution structure. Antibodies raised against rBN28 were used to characterize the recombinant and native proteins. Similar to many other lowtemperature-induced proteins, BN28 is extremely hydrophilic, such that it remains soluble following boiling. lmmunoblot analysis of subcellular fractions indicated that BN28 was not strongly associated with cellular membranes and was localized exclusively within the soluble fraction of the cell. Contrary to predicted secondary structure that suggested significant helical content, circular dichroism analysis revealed that rBN28 existed in aqueous solution largely as a random coil. However, the helical propensity of the protein could be demonstrated in the presence of trifluoroethanol. Nuclear magnetic resonance analysis further showed that rBN28 was in fact completely unstructured (1 00% coil) in aqueous solution. Although it had earlier been speculated that BN28-like proteins from Arabi-

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Alterations in Membrane Protein-Profile during Cold Treatment of Alfalfa

Abstract: ABSTRACT

Cited by 16 publications

References 10 publications

Acclimation to freezing temperatures in perennial ryegrass (Lolium perenne)

Acclimation to freezing temperatures in perennial ryegrass (Lolium perenne)

Chilling Injury. A Review of Possible Mechanisms

Purification, Characterization, and Structural Analysis of a Plant Low-Temperature-Induced Protein

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