Alterations in manganese, copper, and zinc contents, and intracellular status of the metal-containing superoxide dismutase in human mesothelioma cells

Hasegawa, Sumitaka; Koshikawa, Michiko; Takahashi, Isao; Hachiya, Misao; Furukawa, Takako; Akashi, Makoto; Yoshida, Satoshi; Saga, Tsuneo

doi:10.1016/j.jtemb.2008.05.001

Cited by 17 publications

(15 citation statements)

References 35 publications

(32 reference statements)

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“…We previously reported that human MM cells had a higher amount of Mn-SOD protein compared with mesothelial cells when assayed by ELISA. 7 In this study, we further evaluated the levels of Mn-SOD mRNA and protein in human MM cells and mesothelial cells and confirmed that Mn-SOD is overexpressed in most human MM cells compared with mesothelial cells. Mn-SOD is an antioxidant enzyme located in mitochondria and probably plays a key role in oxidative stress and tumor cell apoptosis.…”

Section: Discussionsupporting

confidence: 55%

“…7 Others have also reported similar results. [8][9][10][11][12] These results suggested that Mn-SOD is a reliable molecular marker for MM, prompting us to target Mn-SOD in the development of a more selective molecular imaging for MM.…”

mentioning

confidence: 59%

“…7,11 For example, the Mn-SOD level in H226 was estimated to be about 10-fold higher than that in MSTO-211H cells. 7 In this article, we presented the possibility of detection of MM with a high level of Mn-SOD by MEMRI. However, the sensitivity of MEMRI has yet to be determined.…”

Section: Discussionmentioning

confidence: 97%

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Molecular imaging of mesothelioma by detection of manganese‐superoxide dismutase activity using manganese‐enhanced magnetic resonance imaging

Hasegawa

Koshikawa-Yano²,

Saito

et al. 2011

Intl Journal of Cancer

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Malignant mesothelioma (MM) is a fatal malignancy with a rapidly increasing incidence in industrialized countries because of the widespread use of asbestos in the past centuries. Early diagnosis of MM is critical for a better prognosis, but this is often difficult because of the lack of disease-specific diagnostic imaging. Here, we report that manganese-enhanced magnetic resonance imaging (MEMRI) represents a promising approach for a more selective mesothelioma imaging by monitoring a high-level expression of manganese-superoxide dismutase (Mn-SOD), which is observed in many MM. We found that most human MM cells overexpressed Mn-SOD protein compared with human mesothelial cells and that NCI-H226 human MM cells highly expressed Mn-SOD and augmented Mn accumulation when loaded with manganese chloride (MnCl 2 ). The cells showed marked T 1 -signal enhancement on in vitro MRI after incubation with MnCl 2 because of the T 1 shortening effect of Mn 21 . H226subcutaneous tumor was preferentially enhanced compared with a lung adenocarcinoma cell tumor and another human MM cell tumor in MnCl 2 -enhanced T 1 -weighted MR image (T 1 WI), correlating with their respective Mn-SOD expression levels. Moreover, in a more clinically relevant setting, H226 xenografted pleural tumor was markedly enhanced and readily detected by MEMRI using manganese dipyridoxyl diphosphate (MnDPDP), a clinically used contrast agent, as well as MnCl 2 . Therefore, we propose that MEMRI can be a potentially powerful method for noninvasive detection of MM, with high spatial resolution and marked signal enhancement, by targeting Mn-SOD.Malignant mesothelioma (MM) is an asbestos-related malignant tumor originating from mesothelial cells lining pleural and peritoneal cavities. There is a long latency period (more than 30 years) between the asbestos exposure and the onset of the disease, and the number of patients is now rapidly increasing in industrialized countries because of the widespread use and installation of asbestos fibers in the past centuries. Epidemiological studies indicate that the incidence is rapidly increasing worldwide.

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Section: Discussionsupporting

confidence: 55%

mentioning

confidence: 59%

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Molecular imaging of mesothelioma by detection of manganese‐superoxide dismutase activity using manganese‐enhanced magnetic resonance imaging

Hasegawa

Koshikawa-Yano²,

Saito

et al. 2011

Intl Journal of Cancer

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“…Correlation between zinc and iron shows the importance of these two elements in maintaining the health of the epithelium [19,27]. Positive correlation of Mn with Br, Cu, and Fe in case of leukoplakia may indicate the overutilization of Mn, a cofactor Mn-SOD enzyme in the condition of depleted Br, Fe, and Cu in this disease [32]. Positive correlation between Co and Fe in both the diseased conditions stresses on the importance of these two elements in maintaining epithelial integrity [9].…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, data of the present investigation study showed remarkable depletion in concentration of Mn in blood of leukoplakia patients as compared to that of the healthy counterparts. This probably reflects the system's need to utilize Mn for its specific role in combating oxidative stress through its association with Mn-SOD activity [32]. The possible biochemical interpretation of such contrasting data may be related to the sharp difference in the etio-pathogenesis of the two diseases where one is primarily a connective tissue disorder (OSF) while the other starts from the epithelium (LKP).…”

Section: Discussionmentioning

confidence: 99%

Correlation of Trace Elemental Profiles in Blood Samples of Indian Patients with Leukoplakia and Oral Submucous Fibrosis

Ray

Ghosh

Mallick

et al. 2011

Biol Trace Elem Res

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In order to ascertain possible correlation between alterations in trace elemental profile and the progression or regression of two most common potentially malignant disorders affecting oral cavity, namely oral submucous fibrosis and leukoplakia, blood from 60 patients from each group of patients as well from 30 healthy individuals was analyzed for elemental profiling employing EDXRF technique. Out of the 16 detected elements (K, Si, Ca, V, Cr, Ni, Mn, Fe, Cu, Zn, Se, Br, Rb, Sr, Co, and Pb), Mn, Fe, Zn, Br, and Co showed remarkable alteration in their profile in both leukoplakia and oral submucous fibrosis patients with respect to the normal healthy individuals. While Zn, Br, and Fe reflected similar changes--showing gross depletion in both the diseased groups, Mn and Co depicted inverse pattern of alterations in their concentrations in the two types of precancerous disorders when compared to the control subjects.

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Associations between age and 50 trace element contents and relationships in intact thyroid of males

Zaichick

2018

Aging Clin Exp Res

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Alterations in manganese, copper, and zinc contents, and intracellular status of the metal-containing superoxide dismutase in human mesothelioma cells

Cited by 17 publications

References 35 publications

Molecular imaging of mesothelioma by detection of manganese‐superoxide dismutase activity using manganese‐enhanced magnetic resonance imaging

Molecular imaging of mesothelioma by detection of manganese‐superoxide dismutase activity using manganese‐enhanced magnetic resonance imaging

Correlation of Trace Elemental Profiles in Blood Samples of Indian Patients with Leukoplakia and Oral Submucous Fibrosis

Associations between age and 50 trace element contents and relationships in intact thyroid of males

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