Alterations in Lipid Peroxidation and Certain Antioxidant Enzymes in Different Age Groups under Physiological Conditions

Singh, Kuldip; Kaur, Sandeep; Kumari, Kanta; Singh, Gurshawn; Kaur, Amrit Pal

doi:10.1080/09709274.2009.11906203

Cited by 15 publications

(9 citation statements)

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“…ROS are usually derived from abnormally interrupted metabolism of oxygen and is thought to play an important role in oxidative damage to biological macromolecules (Tian et al 1998). Oxygen radical production in the cell increases with age in mammals and insects (Smith et al 1991) as well as in humans (Singh et al 2009). The age-related increase in production of prooxidants is derived from the membrane damage by O 2˙ˉa nd H 2 O 2 (Starke-Reed and Oliver 1989).…”

Section: Discussionmentioning

confidence: 99%

Age-related learning and memory deficits in rats: role of altered brain neurotransmitters, acetylcholinesterase activity and changes in antioxidant defense system

Haider

Saleem

Perveen

et al. 2014

AGE

138

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Oxidative stress from generation of increased reactive oxygen species or free radicals of oxygen has been reported to play an important role in the aging. To investigate the relationship between the oxidative stress and memory decline during aging, we have determined the level of lipid peroxidation, activities of antioxidant enzymes, and activity of acetylcholine esterase (AChE) in brain and plasma as well as biogenic amine levels in brain from Albino-Wistar rats at age of 4 and 24 months. The results showed that the level of lipid peroxidation in the brain and plasma was significantly higher in older than that in the young rats. The activities of antioxidant enzymes displayed an age-dependent decline in both brain and plasma. Glutathione peroxidase and catalase activities were found to be significantly decreased in brain and plasma of aged rats. Superoxide dismutase (SOD) was also significantly decreased in plasma of aged rats; however, a decreased tendency (non-significant) of SOD in brain was also observed. AChE activity in brain and plasma was significantly decreased in aged rats. Learning and memory of rats in the present study was assessed by Morris Water Maze (MWM) and Elevated plus Maze (EPM) test. Short-term memory and long-term memory was impaired significantly in older rats, which was evident by a significant increase in the latency time in MWM and increase in transfer latency in EPM. Moreover, a marked decrease in biogenic amines (NA, DA, and 5-HT) was also found in the brain of aged rats. In conclusion, our data suggest that increased oxidative stress, decline of antioxidant enzyme activities, altered AChE activity, and decreased biogenic amines level in the brain of aged rats may potentially be involved in diminished memory function.

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Section: Discussionmentioning

confidence: 99%

Age-related learning and memory deficits in rats: role of altered brain neurotransmitters, acetylcholinesterase activity and changes in antioxidant defense system

Haider

Saleem

Perveen

et al. 2014

AGE

138

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“…It has been established from various studies that parameters like age and sex account for the variations in MDA, SOD, and GPx. [10] These factors were taken into considerations and study was done in males of same age group only.…”

Section: Discussionmentioning

confidence: 99%

“…[10] Only male subjects were taken for study as 80% of the workers in thermal plant were males. The mean age of the workers was 35 ± 5 years.…”

Section: Methodsmentioning

confidence: 99%

Effect of occupation on lipid peroxidation and antioxidant status in coal-fired thermal plant workers

Kaur

Gill

Gupta

et al. 2013

Int J App Basic Med Res

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Background:Air pollution from coal-fired power units is large and varied, and contributes to a significant number of negative environmental and health effects. Reactive oxygen species (ROS) have been implicated in the pathogenesis of coal dust-induced toxicity in coal-fired power plants.Aim:The aim of the study was to measure free radical damage and the antioxidant activity in workers exposed to varying levels of coal dust.Material and Methods:The study population consisted of workers in coal handling unit, turbine unit, and boiler unit (n = 50 each), working in thermal power plant; and electricians (n = 50) from same department were taken as controls. Lipid peroxidation was measured by malondialdehyde (MDA) levels and antioxidant activity was determined by superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels. Statistical analysis was carried out by Student's unpaired t-test.Result:MDA levels showed significant increase (P > 0.001) in the thermal power plant workers than the electricians working in the city. The levels of SOD and GPx were significantly higher (P > 0.001) in electricians as compared to subjects working in thermal plant. Among the thermal plant workers, the coal handling unit workers showed significant increase (P > 0.001) in MDA and significant decrease in SOD and GPx than the workers of boiler and turbine unit workers.Conclusion:Oxidative stress due to increase in lipid peroxidation and decrease in antioxidant activity results from exposure to coal dust and coal combustion products during thermal plant activities.

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“…In humans, the similar age-associated dynamics of plasma (serum) Gpx activity was found earlier: comparing mean values, Gpx activity decreased less prominently (1.26-fold) from age of 15-30 to 46-65 years [14] and more prominently (1.8-fold) from age of 50-60 to more than 80 years [15]. It is appropriate to mention, that selenium is essential for Gpx3 biosynthesis [16].…”

Section: Introductionmentioning

confidence: 65%

Decreased Activity of Thiol Peroxidase (Glutathione Peroxidase 3) in Blood Plasma as an Indicator of Aging

Razygraev¹

2017

J Gerontol Geriatr Res

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Thiol peroxidases is a superfamily of antioxidant enzymes catalyzing the thiol-dependent reduction of hydroperoxides. Thiol peroxidases of glutathione peroxidase (Gpx) family utilize reduced glutathione (GSH) as a reductant. Early studies recognized that plasma selenium-dependent Gpx (Gpx3) possesses a wide thiol specificity. Our previous study statistically confirmed the ability of rat Gpx3 to utilize reduced homocysteine (Hcy-SH) instead of GSH. Thus, GSH can be completely replaced by Hcy-SH in a reaction mixture, that allows to determine Gpx3 activity with higher selectivity. In present study, the hypothesis of the age-associated decline of thiol peroxidase activity in rat plasma was tested using Hcy-SH as a thiol substrate. The enzymatic activity in 23-24-month-old rats was significantly lower than in 6-8-month-old rats (p=0.0012, Wilcoxon-Mann-Whitney test). These data are in agreement with the results obtained by other authors revealed the diminished total Gpx activity in serum of 24-month-old rats. The progressive aging-associated decline of Gpx activity in human plasma is also known. Gpx3 activity in plasma may be an appropriate indicator of aging process due to its relatively high value in adult, not aged animals and humans, and its subsequent prominent aging-specific decline.

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Alterations in Lipid Peroxidation and Certain Antioxidant Enzymes in Different Age Groups under Physiological Conditions

Cited by 15 publications

References 1 publication

Age-related learning and memory deficits in rats: role of altered brain neurotransmitters, acetylcholinesterase activity and changes in antioxidant defense system

Age-related learning and memory deficits in rats: role of altered brain neurotransmitters, acetylcholinesterase activity and changes in antioxidant defense system

Effect of occupation on lipid peroxidation and antioxidant status in coal-fired thermal plant workers

Decreased Activity of Thiol Peroxidase (Glutathione Peroxidase 3) in Blood Plasma as an Indicator of Aging

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