Alterations in L-Arginine Metabolism After Lung Transplantation~!2009-11-12~!2010-03-25~!2010-05-04~!

Mehl, Anne; Grasemann, Hartmut

doi:10.2174/1875042701002020055

Cited by 2 publications

(3 citation statements)

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“…Previous studies had reported low NO production after lung transplantation in humans [11,40]. Studies on the effects of preservation and lung transplantation on NO in animal models revealed similar results.…”

Section: Discussionsupporting

confidence: 63%

“…Previous studies have suggested that alterations in the l-arginine/NO metabolism may contribute to complications after lung transplantation [11]. For instance, NO released by macrophages and neutrophils may contribute to the pathogenesis of ischemia reperfusion (I/R) injury [12], and the reaction of NO with oxygen species results in the formation of toxic radicals, which are critical in the development of I/R injury and primary graft dysfunction (PGD) [13,14].…”

Section: Introductionmentioning

confidence: 99%

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Increased Arginase Expression and Decreased Nitric Oxide in Pig Donor Lungs after Normothermic Ex Vivo Lung Perfusion

et al. 2020

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An established pig lung transplantation model was used to study the effects of cold ischemia time, normothermic acellular ex vivo lung perfusion (EVLP) and reperfusion after lung transplantation on l-arginine/NO metabolism in lung tissue. Lung tissue homogenates were analyzed for NO metabolite (NOx) concentrations by chemiluminescent NO-analyzer technique, and l-arginine, l-ornithine, l-citrulline and asymmetric dimethylarginine (ADMA) quantified using liquid chromatography-mass spectrometry (LC-MS/MS). The expression of arginase and nitric oxide synthase (NOS) isoforms in lung was measured by real-time polymerase chain reaction. EVLP preservation resulted in a significant decrease in concentrations of NOx and l-citrulline, both products of NOS, at the end of EVLP and after reperfusion following transplantation, compared to control, respectively. The ratio of l-ornithine over l-citrulline, a marker of the balance between l-arginine metabolizing enzymes, was increased in the EVLP group prior to reperfusion. The expression of both arginase isoforms was increased from baseline 1 h post reperfusion in EVLP but not in the no-EVLP group. These data suggest that EVLP results in a shift of the l-arginine balance towards arginase, leading to NO deficiency in the lung. The arginase/NOS balance may, therefore, represent a therapeutic target to improve lung quality during EVLP and, subsequently, transplant outcomes.

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Section: Discussionsupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Increased Arginase Expression and Decreased Nitric Oxide in Pig Donor Lungs after Normothermic Ex Vivo Lung Perfusion

et al. 2020

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“…Increased arginase activity contributes to pathology after solid organ transplantation, and, in lung transplant recipients, arginase may contribute to organ dysfunction and impact on allograft perfusion, ventilation, acute rejection and graft remodeling [6].…”

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confidence: 99%

Hot Topic: [The Role of Arginase in Lung Disease (Guest Editor: Hartmut Grasemann)]

Grasemann¹

2010

TONOJ

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Nitric oxide synthases (NOSs) metabolize the amino acid L-arginine to form nitric oxide (NO) and L-citrulline. NO is involved in a broad spectrum of processes in the lungs and airways, including perfusion, ventilation, inflammation, infection and airway clearance. The activity of NOS can change in response to, for instance, inflammatory stimuli that can alter enzyme expression. In addition, NOS activity can be regulated post-transcriptionally by specific endogenous NOS inhibitors, such as asymmetric dimethylarginine (ADMA), but also by substrate limitation. The arginase isoenzymes decrease NO production by reducing the availability of L-arginine for NOS [1]. There is accumulating evidence that disturbances of the balance between the L-arginine metabolizing enzymes can result in both acute and chronic pathological changes. Reduced substrate availability for NOS affects the regulation of airways smooth muscle tone for instance in asthma methacholine responsiveness, and downstream products of arginase activity i.e., L-proline and the polyamines, may contribute to lung fibrosis and airway

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Alterations in L-Arginine Metabolism After Lung Transplantation~!2009-11-12~!2010-03-25~!2010-05-04~!

Cited by 2 publications

References 100 publications

Increased Arginase Expression and Decreased Nitric Oxide in Pig Donor Lungs after Normothermic Ex Vivo Lung Perfusion

Increased Arginase Expression and Decreased Nitric Oxide in Pig Donor Lungs after Normothermic Ex Vivo Lung Perfusion

Hot Topic: [The Role of Arginase in Lung Disease (Guest Editor: Hartmut Grasemann)]

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