Alterations in human epidermal Langerhans cells by ultraviolet radiation: quantitative and morphological study

Seité, Sophie; Zucchi, Hélène; Moyal, Dominique; Tison, Sylvie; Compan, Delphine; Christiaens, François; Guéniche, Audrey; Fourtanier, Anny

doi:10.1046/j.1365-2133.2003.05112.x

Cited by 100 publications

(82 citation statements)

References 23 publications

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“…Regardless of the in vitro or in vivo scenario, activation of the immune response by irradiated DC may be significantly altered. Although UV is known to suppress the function of some DC subsets (Schmitt and Ullrich, 2000;Denfeld et al, 2001;Simon et al, 2002;Seite et al, 2003), the effects of ionising radiation have not been fully elucidated, and are often assumed to be immunologically neutral. This study has shown that irradiation of DC significantly affects their behaviour and functional interaction with responding T cells.…”

Section: Discussionmentioning

confidence: 99%

Immunosuppressive effects of radiation on human dendritic cells: reduced IL-12 production on activation and impairment of naïve T-cell priming

et al. 2005

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Dendritic cells (DC) are professional antigen-presenting cells (APC) of the immune system, uniquely able to prime naïve T-cell responses. They are the focus of a range of novel strategies for the immunotherapy of cancer, a proportion of which include treating DC with ionising radiation to high dose. The effects of radiation on DC have not, however, been fully characterised. We therefore cultured human myeloid DC from CD14 þ precursors, and studied the effects of ionising radiation on their phenotype and function. Dendritic cells were remarkably resistant against radiation-induced apoptosis, showed limited changes in surface phenotype, and mostly maintained their endocytic, phagocytic and migratory capacity. However, irradiated DC were less effective in a mixed lymphocyte reaction, and on maturation produced significantly less IL-12 than unirradiated controls, while IL-10 secretion was maintained. Furthermore, peptide-pulsed irradiated mature DC were less effective at naïve T-cell priming, stimulating fewer effector cells with lower cytotoxicity against antigen-specific targets. Hence irradiation of DC in vitro, and potentially in vivo, has a significant impact on their function, and may shift the balance between T-cell activation and tolerisation in DC-mediated immune responses.

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Section: Discussionmentioning

confidence: 99%

Immunosuppressive effects of radiation on human dendritic cells: reduced IL-12 production on activation and impairment of naïve T-cell priming

et al. 2005

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“…Similarly, single doses of UVA1 caused also a decrease in the number of LCs in human epidermis [7,8].…”

Section: Effects Of Uv Radiation On the Number Of Lcs In The Epidermismentioning

confidence: 99%

“…buttocks). Other authors also showed a reduced number of LCs in the epidermis caused by solar simulated radiation (SSR) -UVA + UVB, 290-400 nm -both in humans after exposure to a single dose of UV-SSR (2 MED, 3 MED) [6,7] and in mice after multiple suberythematous doses of irradiation (3.7 J/cm 2 , from 10 to 60 days) [13]. According to Novakovic et al's study, the decrease in the number of LCs was the greatest 72 hours after irradiation with 2 MEDs of SSR [6].…”

Section: Effects Of Uv Radiation On the Number Of Lcs In The Epidermismentioning

confidence: 99%

“…It should be noted that the reduction in the number of LCs in the epidermis after SSR, UVA, UVA1 and UVB irradiation in the majority of experiments was found to be dose dependent [6,7,20].…”

Section: Effects Of Uv Radiation On the Number Of Lcs In The Epidermismentioning

confidence: 99%

“…Numerous studies showed a decrease in the number of LCs in the epidermis caused by exposure to UV radiation, including sunlight [5], solar simulated radiation (SSR) [6], UVA [7,8] and UVB [9,10]. Two mechanisms are considered responsible for this effect: mobilization and migration of LCs to the lymph nodes or induction of necrosis or apoptosis processes by UV [9][10][11][12].…”

Section: Effects Of Uv Radiation On the Number Of Lcs In The Epidermismentioning

confidence: 99%

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Effects of ultraviolet radiation on Langerhans cells

Chomiczewska-Skóra¹,

Adamus²,

Trznadel-Grodzka³

et al. 2013

cejoi

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Research on penetration of ultraviolet (UV) radiation into human skin shows that about 20% of UVA and 10% of UVB can reach the basal layer of the epidermis. As a consequence, Langerhans cells (LCs), located in suprabasal layers, are subject to the influence of the two types of UV radiation. This phenomenon leads to quantitative and qualitative alterations in LCs, including reduction in their number in the epidermis and modifications in morphology and immune functions. Studies show that two mechanisms may be responsible for their depletion from epidermis, namely, migration to the lymph nodes or induction of cell necrosis or apoptosis. UV radiation also affects the phenotype of LCs and their functions as antigen presenting cells. In recent years, the role of LCs in the UV-dependent immunosuppression and immune tolerance has been emphasized. The aim of the study is to review the literature about the influence of ultraviolet radiation on Langerhans cells.

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Topical Therapy

Berth‐Jones¹

2004

Rook's Textbook of Dermatology

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Alterations in human epidermal Langerhans cells by ultraviolet radiation: quantitative and morphological study

Abstract: A single exposure of human skin in vivo to UV-SSR, UVA or UVA1 radiation results in different alterations of density and/or morphology of LCs. All these alterations may impair the antigen-presenting function of LCs leading to an alteration of immune response.

Cited by 100 publications

References 23 publications

Immunosuppressive effects of radiation on human dendritic cells: reduced IL-12 production on activation and impairment of naïve T-cell priming

Immunosuppressive effects of radiation on human dendritic cells: reduced IL-12 production on activation and impairment of naïve T-cell priming

Effects of ultraviolet radiation on Langerhans cells

Topical Therapy

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