Alterations in Haemolymph Proteins of Drone Honey Bee Larvae Parasitized by Varroa Jacobsoni

Gliński, Z.; Jarosz, J.

doi:10.1051/apido:19840305

Cited by 22 publications

(10 citation statements)

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“…Besides brood hemolymph proteins, the detection of honeybee vitellogenin in Varroa is an example of protein that corresponds to the composition of adult bee hemolymph 16 17 18 rather than to larval and pupal hemolymph 13 15 18 . Together, these results indicate that although our Varroa samples were collected from hive bees, the proteins detected in Varroa originated largely from brood hemolymph, consistent with adult bees acting primarily as an intermediate host and a means of transport for Varroa 76 77 .…”

Section: Discussionsupporting

confidence: 53%

“…Varroa mites are parasites of adult bees and their brood. However, Varroa parasitization nearly always takes place in the older brood, in which the adult bee primarily acts as an intermediate host and a means of transport for Varroa 76 77 . The protein with the highest spectral counts, apolipophorin (gi|571543905), is a highly abundant protein that is present at approximately equal levels in all castes, worker adults and worker larvae 18 .…”

Section: Discussionmentioning

confidence: 99%

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In-depth proteomic analysis of Varroa destructor: Detection of DWV-complex, ABPV, VdMLV and honeybee proteins in the mite

Erban

Harant

Hubálek

et al. 2015

Sci Rep

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We investigated pathogens in the parasitic honeybee mite Varroa destructor using nanoLC-MS/MS (TripleTOF) and 2D-E-MS/MS proteomics approaches supplemented with affinity-chromatography to concentrate trace target proteins. Peptides were detected from the currently uncharacterized Varroa destructor Macula-like virus (VdMLV), the deformed wing virus (DWV)-complex and the acute bee paralysis virus (ABPV). Peptide alignments revealed detection of complete structural DWV-complex block VP2-VP1-VP3, VDV-1 helicase and single-amino-acid substitution A/K/Q in VP1, the ABPV structural block VP1-VP4-VP2-VP3 including uncleaved VP4/VP2, and VdMLV coat protein. Isoforms of viral structural proteins of highest abundance were localized via 2D-E. The presence of all types of capsid/coat proteins of a particular virus suggested the presence of virions in Varroa. Also, matches between the MWs of viral structural proteins on 2D-E and their theoretical MWs indicated that viruses were not digested. The absence/scarce detection of non-structural proteins compared with high-abundance structural proteins suggest that the viruses did not replicate in the mite; hence, virions accumulate in the Varroa gut via hemolymph feeding. Hemolymph feeding also resulted in the detection of a variety of honeybee proteins. The advantages of MS-based proteomics for pathogen detection, false-positive pathogen detection, virus replication, posttranslational modifications, and the presence of honeybee proteins in Varroa are discussed.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

In-depth proteomic analysis of Varroa destructor: Detection of DWV-complex, ABPV, VdMLV and honeybee proteins in the mite

Erban

Harant

Hubálek

et al. 2015

Sci Rep

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“…This is supported by the observation of Badotra et al (2009) who also reported that protein fractions established by SDS -PAGE were 12 in infested pupa and 10 in non-infested pupa of A. mellifera. This observation further gets support from the report of Glinski and Jarosz (1984) who described that the host increases production of certain proteins and immune polypeptides in response to parasitism. Thus while total protein concentration decreased, types of proteins synthesised increased.…”

Section: Resultssupporting

confidence: 77%

Changes in protein profile and RNA content of Apis mellifera worker pupa on parasitization with Tropilaelaps clareae

Negi

Kumar

2014

JANS

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Tropilaelaps infestation of Apis mellifera pupa caused reduction in total protein concentration of the infested pupa. This may be due to protein feeding by mite. The additional protein fractions observed by SDS-PAGE and the difference in the nature of free amino acids suggested synthesis of newer proteins perhaps related to the stress response of the host. This opinion is strengthened by the increase in concentration of RNA ( 184.580±36.987 in non-infested pupa and 293.402±50.329 in infested pups) observed in the present study which was responsible for increased transcription of genes encoding antiparasite peptides as reported by other workers.

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“…The mechanism by which DWV effects longevity is currently unknown, but virus replication often shuts down the normal protein production of an infected cell. This may explain the reduced protein content of haemolymph observed in infested bees (Glinski and Jarosz, 1984;Schatton-Gadelmayer and Engels, 1988), and this could affect longevity. However, longevity of adult honey bees is unaffected when bees are infected during the adult stage ( Fig.…”

Section: Discussionmentioning

confidence: 99%

The role of deformed wing virus in the initial collapse of varroa infested honey bee colonies in the UK

Martin

Ball

Carreck

2013

Journal of Apicultural Research

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SummaryThe mite Varroa destructor has been associated with the collapse of millions of Apis mellifera honey bee colonies world-wide. During the past decade, a large body of research has revealed various interactions between varroa, the honey bee and various viral pathogens. One pathogen in particular, deformed wing virus (DWV), has emerged as the key pathogen involved in colony collapse. As varroa has permanently changed the viral landscape in which honey bees exist, we present a large body of data on the effects of DWV during the initial phase of varroa infestation in the UK during 1998. This provides baseline data for further comparative studies. We carried out DWV transmission studies, and observed the effects of DWV on bee longevity. As the ELISA technique used in these studies had a detection limit of ~10 7 viral particles per bee, only high viral (overt) titres were detected. During the initial phase of varroa establishment, DWV was detected in 0.6% of non-infested sealed brood, but in 89% of sealed brood invaded by varroa. Once DWV was introduced into the bee's haemolymph via mite feeding on either pupae or adults, an overt virus infection was rapidly produced in 3-4 days. In sealed brood the presence of varroa was fatal for 21% of the brood, caused wing deformity in some emerging adults and significantly reduced longevity as an adult. However, adult bees that became infected after they had emerged, did not develop wing deformity nor show any reduced longevity, but acted as reservoirs of DWV infection. El papel de los virus de las alas deformadas en el colapso inicial de las colonias de abejas infestadas por varroa en el Reino Unido ResumenEl ácaro Varroa destructor se ha asociado con el colapso de millones de colonias de abejas Apis mellifera en todo el mundo. Durante la última década, un amplio volumen de investigaciones ha puesto de manifiesto diversas interacciones entre varroa, la abeja de la miel y diversos patógenos virales. Un patógeno en particular, el virus de las alas deformadas (DWV), se ha convertido en el agente patógeno clave implicado en el colapso de colonias. Como varroa ha cambiado para siempre el paisaje viral en el que existen las abejas, se presenta aquí una gran cantidad de datos sobre los efectos del DWV durante la fase inicial de la infestación de varroa en el Reino Unido durante 1998. Esto proporciona datos de referencia para futuros estudios comparativos. Hemos llevado a cabo estudios de transmisión del DWV, y observamos los efectos del DWV en la longevidad de la abeja. Como la técnica de ELISA usada en estos estudios tenía un límite de detección de ~ 107 partículas virales por abeja, solamente se detectaron concentraciones virales altas (manifestadas). Durante la fase inicial del establecimiento de varroa, el DWV se detectó en el 0,6% de la cría operculada no infestada, y en el 89% de la cría operculada invadida por varroa. Una vez que el DWV se introdujo en la hemolinfa de la abeja a través de la alimentación del ácaro tanto en pupas como en adultos, la infección manifestada p...

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Alterations in Haemolymph Proteins of Drone Honey Bee Larvae Parasitized by Varroa Jacobsoni

Cited by 22 publications

References 0 publications

In-depth proteomic analysis of Varroa destructor: Detection of DWV-complex, ABPV, VdMLV and honeybee proteins in the mite

In-depth proteomic analysis of Varroa destructor: Detection of DWV-complex, ABPV, VdMLV and honeybee proteins in the mite

Changes in protein profile and RNA content of Apis mellifera worker pupa on parasitization with Tropilaelaps clareae

The role of deformed wing virus in the initial collapse of varroa infested honey bee colonies in the UK

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