Alterations in fatty acid composition and fluidity of cell membranes affect the accumulation of PCB congener 2,2′,5,5′‐tetrachlorobiphenyl by <i>Ralstonia eutropha</i> H850

Kim, In Soo; Beaudette, Lee A.; Cassidy, Mike; Lee, Hung; Trevors, J. T.

doi:10.1002/jctb.632

Cited by 23 publications

(9 citation statements)

References 36 publications

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“…After measuring FL polarization, the FL polarizations ( P ) of the untreated and treated E. coli by lysozyme were 0.32 and 0.38, respectively. The greater the value of P , the lower the membrane fluidity is and vice versa. − Generally speaking, the membrane fluidity is also lipid fluidity . DPH can easily insert into lipid, so the fluorescence polarization can reflect the fluidity characteristics of the lipid (membrane).…”

Section: Resultsmentioning

confidence: 99%

Studies on CdSe/l-cysteine Quantum Dots Synthesized in Aqueous Solution for Biological Labeling

Liu

Wang

2009

J. Phys. Chem. C

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Water-soluble, biologically compatible CdSe quantum dots (QDs) with l-cysteine as capping agent were synthesized in aqueous medium. Fluorescence (FL) spectra, absorption spectra, and transmission electron microscopy studies showed that both the molar ratio of Se/Cd and the reaction time are the determining factors for the size distribution of CdSe/l-cysteine QDs. The interaction of QDs bioconjugated to bovine serum albumin (BSA) was also studied by absorption and FL titration experiments. With addition of QDs, the FL intensity of BSA was largely quenched, which can be explained by static mechanism. However, when BSA was added to the solution of QDs, FL intensity of QDs was faintly enhanced at first and then quenched. Lysozyme could change the Escherchia coli (E. coli) membrane fluidity and permeability by FL polarimetry. Fluorescent imaging suggested that QDs can be designed as a probe to label the E. coli cell. These results suggested CdSe/l-cysteine QDs can be used as a probe for labeling biological molecule and bacteria cell.

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Section: Resultsmentioning

confidence: 99%

Studies on CdSe/l-cysteine Quantum Dots Synthesized in Aqueous Solution for Biological Labeling

Liu

Wang

2009

J. Phys. Chem. C

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“…The results suggest that strain DHM-1T modified its membrane composition to compensate for the fluidizing effects of CF. An increase in the saturated fatty acid component and concomitant decrease in membrane fluidity in Ralstonia eutropha were observed as an adaptation to the fluidizing effects of biphenyl (27,28).…”

Section: Discussionmentioning

confidence: 96%

Anaerobic Biotransformation of High Concentrations of Chloroform by an Enrichment Culture and Two Bacterial Isolates

Shan¹,

Kurtz

Mykytczuk

et al. 2010

Appl Environ Microbiol

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A fermentative enrichment culture (designated DHM-1) was developed that is capable of cometabolically biotransforming high concentrations of chloroform (CF) to nontoxic end products. Two Pantoea spp. were isolated from DHM-1 that also possess this dechlorination capability. Following acclimation to increasing levels of CF, corn syrup-grown DHM-1 was able to transform over 500 mg/liter CF in the presence of vitamin B 12 (approximately 3% of CF on a molar basis) at a rate as high as 22 mg/liter/day in a mineral salts medium. CO, CO 2 , and organic acids were the predominant biodegradation products, suggesting that hydrolytic reactions predominate during CF transformation. DHM-1 was capable of growing on corn syrup in the presence of high concentrations of CF (as may be present near contaminant source zones in groundwater), which makes it a promising culture for bioaugmentation. Strains DHM-1B and DHM-1T transform CF at rates similar to that of the DHM-1 enrichment culture. The ability of these strains to grow in the presence of high concentrations of CF appears to be related to alteration of membrane fluidity or homeoviscous and homeophasic adaptation.Chloroform (CF) is a toxic organic compound that is frequently detected in groundwater. In the 2007 "CERCLA Priority List of Hazardous Substances," CF ranks eleventh overall and is the third highest among chlorinated organics after vinyl chloride and polychlorinated biphenyls (4). When present at hazardous waste sites, CF is often a focal point for evaluating the feasibility of bioremediation, since it is toxic to many obligate anaerobic prokaryotes (44). For example, 1 mg/liter of CF completely inhibited dechlorination of tetrachloroethene (PCE) by a chlororespiring anaerobic isolate (32). Inhibition of reductive dechlorination of chloroethenes by CF is a general problem for sites cocontaminated with CF, which can only be overcome by first removing the CF (6).In spite of major recent advances in bioremediation of chlorinated organic compounds, treatment of CF, especially at high concentrations (e.g., Ͼ100 mg/liter), remains challenging. Although aerobic biotransformation of CF is possible (e.g., cometabolism by a butane-grown strain) (14), CF is more difficult to cometabolize than trichloroethene (42). Biotransformation of CF by mixed or pure cultures under methanogenic (5, 21) and sulfate-reducing (20) conditions has been reported, however, only at low-mg/liter CF concentrations.Corrinoids such as vitamin B 12 (i.e., cyanocobalamin) are effective catalysts for increasing the rate of halomethane biotransformation under anaerobic conditions. Addition of vitamin B 12 also shifts the pathway away from reductive dechlorination and toward hydrolytic and substitutive reactions, forming CO, CO 2 , and organic acids as the major products (8,23,24). With low levels of B 12 added (3 to 5% molar ratios of CF), an enrichment culture grown on dichloromethane (DCM) as the sole substrate (8) and a lactate-grown sulfate-reducing enrichment culture (18) were able to biotransform up ...

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“…Sensitivity is likely to be an important issue when the available labeled compounds are present in very low concentrations (44). For that reason, lipid SIP offers the most appropriate approach when monitoring pollutant degradation processes in natural environments (24,28,29,35), although it is phylogenetically less specific than DNA or RNA SIP (31).…”

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confidence: 99%

Effect of Glucose on the Fatty Acid Composition of Cupriavidus necator JMP134 during 2,4-Dichlorophenoxyacetic Acid Degradation: Implications for Lipid-Based Stable Isotope Probing Methods

Lerch

Dignac

Barriuso

et al. 2011

Appl Environ Microbiol

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Combining lipid biomarker profiling with stable isotope probing (SIP) is a powerful technique for studying specific microbial populations responsible for the degradation of organic pollutants in various natural environments. However, the presence of other easily degradable substrates may induce significant physiological changes by altering both the rate of incorporation of the target compound into the biomass and the microbial lipid profiles. In order to test this hypothesis, Cupriavidus necator JMP134, a 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacterium, was incubated with [ 13 C]2,4-D, [ 13 C]glucose, or mixtures of both substrates alternatively labeled with 13 C. C. necator JMP134 exhibited a preferential use of 2,4-D over glucose. The isotopic analysis showed that glucose had only a small effect on the incorporation of the acetic chain of 2,4-D into the biomass (at days 2 and 3) and no effect on that of the benzenic ring. The addition of glucose did change the fatty acid methyl ester (FAME) composition. However, the overall FAME isotopic signature reflected that of the entire biomass. Compound-specific individual isotopic analyses of FAME composition showed that the 13 C-enriched FAME profiles were slightly or not affected when tracing the 2,4-D acetic chain or 2,4-D benzenic ring, respectively. This batch study is a necessary step for validating the use of lipid-based SIP methods in complex environments.

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Alterations in fatty acid composition and fluidity of cell membranes affect the accumulation of PCB congener 2,2′,5,5′‐tetrachlorobiphenyl by Ralstonia eutropha H850

Cited by 23 publications

References 36 publications

Studies on CdSe/l-cysteine Quantum Dots Synthesized in Aqueous Solution for Biological Labeling

Studies on CdSe/l-cysteine Quantum Dots Synthesized in Aqueous Solution for Biological Labeling

Anaerobic Biotransformation of High Concentrations of Chloroform by an Enrichment Culture and Two Bacterial Isolates

Effect of Glucose on the Fatty Acid Composition of Cupriavidus necator JMP134 during 2,4-Dichlorophenoxyacetic Acid Degradation: Implications for Lipid-Based Stable Isotope Probing Methods

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