Alterations in erythrocyte membrane fluidity and Na+/K+-ATPase activity in chronic alcoholics

Maturu, Paramahamsa; Reddy, Vaddi Damodara; Padmavathi, P.; Nallanchakravarthula, Varadacharyulu

doi:10.1007/s11010-009-0367-z

Cited by 34 publications

(30 citation statements)

References 31 publications

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“…Alterations in the Chol/PL ratio homeostasis may cause an abnormal function and pathological process [19] . The increased Chol/PL ratio in this study confirms alterations in membrane organisation leading to decreased fluidity, low permeability, and altered stability and morphology [56] . These findings also support the hypothesis of Chochina et al [51] on the role of Aβ peptides in neuronal membrane fluidity, that membrane perturbation is associated with cholesterol content.…”

Section: Discussionsupporting

confidence: 71%

Dietary Selenium or Zinc Supplementation Restores Brain Lipid Composition and Membrane Fluidity in Protein-Undernourished Rats

Adebayo

Salau²,

Sandhir³

et al. 2016

Dev Neurosci

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Studies have shown that protein undernutrition (PU) modifies the membrane lipid composition in the intestine and liver, as well as in plasma and other areas. However, there is limited information on the effect of PU on synaptosomal membrane lipid composition and fluidity and the protective role of selenium (Se) and zinc (Zn), which is a major focus of the present study. For 10 weeks, rats were fed diets containing 16% casein, which constituted the adequate protein diet, or 5% casein, representing the PU diet. The animals were supplemented with Se and Zn at a concentration of 0.15 and 227 mg L^-1, respectively, in drinking water for 3 weeks. The results showed a significant increase in total lipids, glycolipids, triglycerides, cholesterol, and the cholesterol/phospholipid (Chol/PL) ratio, and a significant reduction in phospholipids and membrane fluidity. Se and Zn supplementation to PU rats, however, significantly lowered total lipids, glycolipids, triglycerides, cholesterol, and the Chol/PL ratio, while phospholipids and membrane fluidity were significantly restored. It is concluded that a perturbed lipid composition induced by PU affects the membrane structure and fluidity, which in turn influences membrane functions. The study suggests that Se and Zn supplementation might be beneficial in restoring the lipid dyshomeostasis associated with PU.

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Section: Discussionsupporting

confidence: 71%

Dietary Selenium or Zinc Supplementation Restores Brain Lipid Composition and Membrane Fluidity in Protein-Undernourished Rats

Adebayo

Salau²,

Sandhir³

et al. 2016

Dev Neurosci

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“…The peroxidation of lipids in patients could indicate an injury of membranes by oxidative stress [13]. An alcohol-induced overproduction of nitric oxide, which reacts with superoxide radicals to synthesise peroxynitrite, might also represent one of the many possible causes for the membrane damage [14]. …”

Section: Introductionmentioning

confidence: 99%

Elevated oleic acid serum concentrations in patients suffering from alcohol dependence

et al. 2013

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BackgroundAlcohol-induced damages such as brain atrophy and fatty liver are closely related to a disturbed lipid metabolism. In animal models, a linkage between chronic alcohol consumption and changes in fatty acid (FA) composition in various organs and cells is well known and there is some indication that this phenomenon could be linked to behavioural alterations associated with alcohol addiction such as craving. However, the influence of ethanol on secretory FA has not been investigated so far. In this study, we therefore aimed at investigating whether there is a significant change of serum FA composition in patients suffering from alcohol dependence. We compared patients before and after treatment (detoxication) with control individuals who did not suffer from addiction. The roles of age, the duration and intensity of alcohol use and lifestyles were considered.MethodsSerum FA was measured in 73 male ethanol dependent patients before and after alcohol withdrawal in an in-patient setting. Additionally, of this group, 45 patients were matched with 45 healthy male volunteers as controls.ResultsWe found significant differences in the FA composition before and after detoxication as well as between patients and controls. After detoxication, the values changed towards the ones in healthy controls. The main finding during acute alcohol use was an increased oleic acid concentration above the level of the linoleic acid concentration.ConclusionsAn elevated oleic/linoleic acid ratio seems to be a state marker for acute alcohol use and may be a relevant trait marker during detoxification and possibly the subsequent therapeutic measures. The results of this pilot study need to be replicated in a larger study also including female patients. Further, the specificity of this potential biomarker needs to be determined.

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“…Such resulting membrane rigidification potentially resists the membrane-fluidizing effects of local anesthetics. In alcoholic patients, erythrocyte membranes show a decrease in membrane fluidity or a pharmacodynamic tolerance to alcohol together with altering the membrane lipid composition [20,25]. Besides the membrane adaptation to alcohol, THH is able to physicochemically modify neuronal membranes.…”

Section: Discussionmentioning

confidence: 99%

Drinking-Related Tetrahydroharmans Counteract the Membrane Effects of Local Anesthetic Lidocaine

Tsuchiya

Mizogami²

2014

J Drug Alcohol Res

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There is a general consensus in dentistry that successful local anesthesia is frequently difficult in habitual drinkers and alcoholic patients. Neuro-active tetrahydroharmans increase in human body fluids and tissues by consuming alcoholic beverages. To understand such reduced anesthetic efficacy by the drug interaction hypothesis, we studied the influences of drinking-related tetrahydroharmans on membrane fluidization as one of local anesthetic mechanisms. Liposomal membranes prepared with phosphatidylcholine and cholesterol were treated with lidocaine and different tetrahydroharmans separately and in combination, followed by measuring fluorescence polarization to determine their induced changes in membrane fluidity. In contrast to 0.1-2 mg/mL lidocaine, tetrahydroharmans decreased the fluidity of membrane preparations at ∼ 25 μg/mL with the potency being 1,2,3,4-tetrahydroharman 1,2,3,4-tetrahydronorharman. 1,2,3,4-Tetrahydroharman counteracted the membrane-fluidizing effects of 1 mg/mL lidocaine at physiologically relevant 0.25-2.5 ng/mL, whereas neither its 6-hydroxyl nor 7-hydroxyl metabolite did at 25-200 ng/mL. Such counteraction at a membrane lipid level may be responsible for the reduction of local anesthetic efficacy in drinkers because 1,2,3,4-tetrahydroharman increases in vivo by ingesting alcoholic beverages.

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Alterations in erythrocyte membrane fluidity and Na+/K+-ATPase activity in chronic alcoholics

Cited by 34 publications

References 31 publications

Dietary Selenium or Zinc Supplementation Restores Brain Lipid Composition and Membrane Fluidity in Protein-Undernourished Rats

Dietary Selenium or Zinc Supplementation Restores Brain Lipid Composition and Membrane Fluidity in Protein-Undernourished Rats

Elevated oleic acid serum concentrations in patients suffering from alcohol dependence

Drinking-Related Tetrahydroharmans Counteract the Membrane Effects of Local Anesthetic Lidocaine

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