Alterations in co-abundant bacteriome in colorectal cancer and its persistence after surgery: a pilot study

Png, Chin-Wen; Chua, Yong-Kang; Law, Jia-Hao; Zhang, Yongliang; Tan, Ker‐Kan

doi:10.1038/s41598-022-14203-z

Cited by 12 publications

(7 citation statements)

References 70 publications

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“…Similar evidence was suggested by the Malaysian population studied by Png et al [ 53 ]. They evaluated forty-nine fecal samples from 25 noncancer (NC) individuals and 12 CRC patients, before and 6 months after surgery, performing analysis through bacterial 16S rRNA gene sequencing.…”

Section: Which Microbes and Which Molecular Pathways After Gastrointe...supporting

confidence: 90%

Dysbiosis and Gastrointestinal Surgery: Current Insights and Future Research

et al. 2022

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Surgery of the gastrointestinal tract can result in deep changes among the gut commensals in terms of abundance, function and health consequences. Elective colorectal surgery can occur for neoplastic or inflammatory bowel disease; in these settings, microbiota imbalance is described as a preoperative condition, and it is linked to post-operative complications, as well. The study of bariatric patients led to several insights into the role of gut microbiota in obesity and after major surgical injuries. Preoperative dysbiosis and post-surgical microbiota reassessment are still poorly understood, and they could become a key part of preventing post-surgical complications. In the current review, we outline the most recent literature regarding agents and molecular pathways involved in pre- and post-operative dysbiosis in patients undergoing gastrointestinal surgery. Defining the standard method for microbiota assessment in these patients could set up the future approach and clinical practice.

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Section: Which Microbes and Which Molecular Pathways After Gastrointe...supporting

confidence: 90%

Dysbiosis and Gastrointestinal Surgery: Current Insights and Future Research

et al. 2022

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“…Our results are consistent with previous findings of associations of GM in tumorigenesis. In particular, our cohorts highlighted an increase in Prevotella, Fusobacteria, and Bacteroides in BC patients, all widely reported to be associated both with tumorigenesis and response to therapy [19][20][21][22]. In addition to individual associations, our real-world cohorts from both institutions showed similar clustering patterns across communities (groups I-III) and association with chemotherapy response.…”

Section: Discussionsupporting

confidence: 60%

Role of Gut Microbiome in Neoadjuvant Chemotherapy Response in Urothelial Carcinoma: A Multi-Institutional Prospective Cohort Evaluation

Bukavina

Ginwala

Sindhani

et al. 2023

Preprint

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Treatment with neoadjuvant chemotherapy (NAC) in muscle invasive bladder cancer (MIBC) is associated with clinical benefit in urothelial carcinoma. While extensive research evaluating role of tumor mutational expression profiles and clinicopathologic factors into chemoresponse has been published, the role of gut microbiome (GM) in bladder cancer in chemoresponse has not been thoroughly evaluated. A working knowledge of the microbiome and its effect on all forms of cancer therapy in BC is critical. Here we examine gut microbiome of bladder cancer patients undergoing NAC. Overall, there was no significant difference in alpha and beta diversity by responder status. However, analysis of fecal microbiome samples showed that a higher abundance of Bacteroides within both institutional cohorts during NAC was associated with residual disease at the time of radical cystectomy regardless of chemotherapy regimen. Group community analysis revealed presence of favorable microbial subtypes in complete responders. Finally, fecal microbial composition outperformed clinical variables in prediction of complete response (AUC 0.88 vs AUC 0.50), however, no single microbial species could be regarded as a fully consistent biomarker. Microbiome-based community signature as compared to single microbial species is more likely to be associated as the link between bacterial composition and NAC response.

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“…In the present study, three other Bacteroides species were increased in polyps and cancers compared to healthy controls. B. vulgatus has been shown to decrease in abundance after CRC surgery and could potentially have a role in CRC [ 45 ]. Yachida et al investigated the tumor microbiome in different stages of CRC and showed that Phascolarctobacterium was increased in early stages of colorectal disease [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Microbial dynamics with CRC progression: a study of the mucosal microbiota at multiple sites in cancers, adenomatous polyps, and healthy controls

Senthakumaran

Moen

Tannæs

et al. 2023

Eur J Clin Microbiol Infect Dis

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Accumulating evidence has related the gut microbiota to colorectal cancer (CRC). Fusobacterium nucleatum has repeatedly been linked to colorectal tumorigenesis. The aim of this study was to investigate microbial composition in different sampling sites, in order to profile the microbial dynamics with CRC progression. Further, we characterized the tumor-associated F. nucleatum subspecies. Here, we conducted Illumina Miseq next-generation sequencing of the 16S rRNA V4 region in biopsy samples, to investigate microbiota alterations in cancer patients, patients with adenomatous polyp, and healthy controls in Norway. Further, Fusobacterium positive tumor biopsies were subjected to MinION nanopore sequencing of Fusobacterium-specific amplicons to characterize the Fusobacterium species and subspecies. We found enrichment of oral biofilm-associated bacteria, Fusobacterium, Gemella, Parvimonas, Granulicatella, Leptotrichia, Peptostreptococcus, Campylobacter, Selenomonas, Porphyromonas, and Prevotella in cancer patients compared to adenomatous polyp patients and control patients. Higher abundance of amplicon sequence variants (ASVs) classified as Phascolarctobacterium, Bacteroides vulgatus, Bacteroides plebeius, Bacteroides eggerthii, Tyzzerella, Desulfovibrio, Frisingicoccus, Eubacterium coprostanoligenes group, and Lachnospiraceae were identified in cancer and adenomatous polyp patients compared to healthy controls. F. nucleatum ssp. animalis was the dominating subspecies. F. nucleatum ssp. nucleatum, F. nucleatum ssp. vincentii, Fusobacterium pseudoperiodonticum, Fusobacterium necrophorum, and Fusobacterium gonidiaformans were identified in five samples. Several biofilm-associated bacteria were enriched at multiple sites in cancer patients. Another group of bacteria was enriched in both cancer and polyps, suggesting that they may have a role in polyp development and possibly early stages of CRC.

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Alterations in co-abundant bacteriome in colorectal cancer and its persistence after surgery: a pilot study

Cited by 12 publications

References 70 publications

Dysbiosis and Gastrointestinal Surgery: Current Insights and Future Research

Dysbiosis and Gastrointestinal Surgery: Current Insights and Future Research

Role of Gut Microbiome in Neoadjuvant Chemotherapy Response in Urothelial Carcinoma: A Multi-Institutional Prospective Cohort Evaluation

Microbial dynamics with CRC progression: a study of the mucosal microbiota at multiple sites in cancers, adenomatous polyps, and healthy controls

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