Alterations in cellular structure of Mytilus edulis resulting from exposure to environmental contaminants under field and experimental conditions

Lowe, DM

doi:10.3354/meps046091

Cited by 132 publications

(79 citation statements)

References 16 publications

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“…This suggests a decrease in TG mobilization to the PH pool with potential consequences on membrane structure and function. Similarly, Lowe (1988) observed changes in the characteristics of the digestive cell lysosomes in mussels exposed to PAHs in Langesundfjord (Norway). These studies showed that PAHs were sequestered in the lysosomes in cells with high lipid content.…”

Section: Biochemical Compositionmentioning

confidence: 99%

Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach

Labarta

Fernández‐Reiriz²,

Garrido³

et al. 2005

Mar. Ecol. Prog. Ser.

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Postlarval recruits of mussels Mytilus galloprovincialis were sampled in February 2003 at 7 localities along the Galicia coast (NW Spain) exhibiting different levels of impact from the 'Prestige' oil spill. These localities are important grounds for seed collection used in raft culture. The concentrations of polycyclic aromatic hydrocarbons (PAHs) as well as different biochemical and ecophysiological variables were determined in mussel tissues, as indicators of the toxic impact of the spilled oil. The following parameters were measured: size frequency distributions and size-weight relationships, survival in air, biochemical composition (proteins, carbohydrates, glycogen and lipids), lipid classes (phospholipids, sterol esters and waxes, triacylglycerols, free fatty acids and sterols), and fatty acid composition. The study determined sub-lethal effects in wild mussel seed populations sampled 3 mo after the oil spill. These effects are shown in the form of survival indices and alterations of lipid metabolism, especially in trygliceride and sterol fractions, as well as in saturated/monounsaturated fatty acid ratios. Monitoring these effects in wild mussel recruits is important for analysing growth, production and biochemical reserve cycle throughout the raft cultivation period and evaluating the capacity of the individuals to repair alterations detected in the juvenile stage. KEY WORDS: Mussel · Postlarvae · 'Prestige' · Toxic impact · PAH · Biochemical analysisResale or republication not permitted without written consent of the publisher

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Section: Biochemical Compositionmentioning

confidence: 99%

Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach

Labarta

Fernández‐Reiriz²,

Garrido³

et al. 2005

Mar. Ecol. Prog. Ser.

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“…Lipofuscin seems to be common in molluscs, as it has been reported in situ in various tissues and organs of several species such as the gastropods Maoricrypta monoxyla (Nelson and Morton, 1979) and Aplysia punctata (Taieb and Vicente, 1999) and the bivalves Mytilus galloprovincialis (Viarengo et al, 1987(Viarengo et al, , 1990Regoli, 1992;Sarasquete et al, 1992), Mytilus edulis (Moore et al, 1980;George et al, 1982;Lowe, 1988;Burbidge et al, 1994;Hole et al, 1995;Krishnakumar et al, 1994) and Perna viridis (Krishnakumar et al, 1990;Mathew and Damodaran, 1997). Only a few findings are reported from clams so far: In the alimentary tract epithelium, in gonadal connective tissue and in the renal epithelium of Mya arenaria and Mercenaria mercenaria (Brown and O'Toole, 1978); in the digestive gland of Ruditapes phillipinarum (Sarasquete et al, 1992) and of Sunetta scripta (Mathew and Damodaran, 1997); and in the kidney of Tridacna gigas (Reid et al, 1984).…”

Section: Is the Substance Found In E Exalbida Lipofuscin?mentioning

confidence: 99%

Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Laudien

Schiedek

Brey

et al. 2002

Journal of Experimental Marine Biology and Ecology

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In the hard clam Eurhomalea exalbida, autofluorescent granules were detected in high concentrations in the connective tissue around the intestine and in other tissues. Autofluorescence combined with Sudan black B and PAS positive reactions suggested that these granules were lipofuscin-like. The concentration of this material in the connective tissue (CT) around the intestine was quantified by image analysis and expressed as total area fraction occupied by lipofuscin granules. Lipofuscin concentration was distinctly better related with individual age as determined from stable isotope-validated shell growth bands than with any morphometric parameter. This relationship was described best by a Gompertz model: Lipofuscin t = 24.79e À e À 0.029(Age t À 58.578) (N = 38; r nl 2 = 0.882). Age was predicted from lipofuscin CT concentration by a von Bertalanffy model: Age t = 68.00 (1 À e À 0.146(Lipofuscin t + 0.028) ) 0.664 (N = 38; r nl 2 = 0.933). Our findings suggest that lipofuscin CT concentration in E. exalbida is a function of individual age. If this holds true for bivalves in general, lipofuscin may be a suitable proxy for age in species with less clear shell growth band patterns. D

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“…그러나 NL 의 세포 내 과잉 축적은 세포의 사멸(cell death) 혹은 세 (Moore 1988;Krishnakumar et al 1994;Kagley et al 2003;Dimitriadis et al 2004). 또 한 항구 주변이나 공업단지 주변에 서식하는 담치의 소화 맹낭에도 LF 축적이 높은 것을 세포화학적인 방법을 통해 많은 연구에서 보고된 바 있다 (Lowe 1988;Moore 1988;Viarengo and Nott 1993;Krishnakumar et al 1994;Kagley et al 2003;Moore et al 2004).…”

Section: 서 론unclassified

“…그러나 조직관찰을 위한 파라 핀 블록 제작은 그 준비 기간이 오래 걸리며, 고정과 포매 과정에서 지질성분 혹은 효소의 활성을 잃어버리는 단점 이 있다 (Fink 1986 (Fink 1986;Lowe 1988;Moore 1988;Krishnakumar et al 1994;Dimitriadis et al 2004). 이와 같은 이유로, 대부분의 해산 이매패류 소화맹 낭 리소솜 활성의 연구는 동결절편 된 조직에서 세포화학 적인 방법을 이용하여 이루어지고 있다 (Lowe 1988;Moore 1988;Krishnakumar et al 1994;Petrovi et al 2001;Kagley et al 2003;Dimitriadis et al 2004).…”

Section: 서 론unclassified

Cytochemical Measurement of Lysosomal Responses in the Digestive Cells of Wild Pacific Oyster, Crassostrea gigas on the South Coast of Korea

Jeung¹,

Lee²,

Hong³

et al. 2012

Ocean and Polar Research

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: Digestive cells of the bivalves have a highly developed lysosomal system and the system is known to be sensitive to changes in environmental qualities. In this study, we measured lysosomal responses of the digestive cells in wild oyster, Crassostrea gigas using frozen section. Oysters were collected in June 2010 from intertidal areas in the inner and outer bay of Gwangyang off the south coast of Korea. From the tissue sections, we measured the digestive cell lysosomal membrane stability (LMS), level of neutral lipids (NL), lipofuscin (LF) and the digestive gland atrophy (DGA). The DGA and condition index of oysters from the inner bay were significantly lower (P<0.05). The statistical test indicated that LMS levels of oysters in the inner bay and the outer bay were not significantly different since a shorter activity was displayed by oysters from the inner bay than that of oysters in outer bay. The LF deposition level of the oysters in the inner bay displayed significantly higher levels than the outer bay (P<0.05). In contrast, the NL accumulation measured from oysters in outer bay was significantly higher than the level observed in the inner bay (P<0.05). Different levels of DGA and LF that were observed in the inner and outer bays were thought to be associated to different level of environmental contamination and these two assays are considered to be good biomarkers.

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Alterations in cellular structure of Mytilus edulis resulting from exposure to environmental contaminants under field and experimental conditions

Cited by 132 publications

References 16 publications

Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach

Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach

Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Cytochemical Measurement of Lysosomal Responses in the Digestive Cells of Wild Pacific Oyster, Crassostrea gigas on the South Coast of Korea

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Alterations in cellular structure of Mytilus edulis resulting from exposure to environmental contaminants under field and experimental conditions

Cited by 132 publications

References 16 publications

Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach

Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach

Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Cytochemical Measurement of Lysosomal Responses in the Digestive Cells of Wild Pacific Oyster, Crassostrea gigas on the South Coast of Korea

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Product

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Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach

Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach