Alterations in cell growth and signaling in ErbB3 binding protein-1 (Ebp1) deficient mice

Zhang, Yuexing; Lu, Yan; Zhou, Hua; Lee, Myoung-Hee; Liu, Zhenqiu; Hassel, Bret A.; Hamburger, Anne W.

doi:10.1186/1471-2121-9-69

Cited by 43 publications

(48 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Loss-of-expression studies in mice and chick indicate a role in regulating proliferation and differentiation during developmental and adult stages of myogenesis (Figeac et al, 2014). Consistent with a role in proliferation, Pa2g4 -deficient mice are 30% smaller compared to wild type littermates and show cellular hallmarks associated with growth retardation (Zhang et al, 2008). Nonetheless, to our knowledge there are no studies of its function in the craniofacial development of any vertebrate.…”

Section: Introductionmentioning

confidence: 92%

“…Pa2G4 also contains distinct motifs in the C-terminus that are involved in RNA binding (Kowalinski et al, 2007; Monie et al, 2007; Squatrito et al, 2004). In mammals, Pa2G4 can interact with a diverse range of proteins including several receptors, kinases and cell cycle regulators (Xia et al, 2001a; Yoo et al, 2000; Zhang et al, 2008). In mammalian cell culture, it associates with the inactive form of the ErbB-3 receptor, and translocates to the nucleus following receptor activation (Yoo et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pa2G4 is a novel Six1 co-factor that is required for neural crest and otic development

Neilson

Abbruzzesse

Kenyon

et al. 2017

Developmental Biology

View full text Add to dashboard Cite

Mutations in SIX1 and in its co-factor, EYA1, underlie Branchiootorenal Spectrum disorder (BOS), which is characterized by variable branchial arch, otic and kidney malformations. However, mutations in these two genes are identified in only half of patients. We screened for other potential co-factors, and herein characterize one of them, Pa2G4 (aka Ebp1/Plfap). In human embryonic kidney cells, Pa2G4 binds to Six1 and interferes with the Six1-Eya1 complex. In Xenopus embryos, knock-down of Pa2G4 leads to down-regulation of neural border zone, neural crest and cranial placode genes, and concomitant expansion of neural plate genes. Gain-of-function leads to a broader neural border zone, expanded neural crest and altered cranial placode domains. In loss-of-function assays, the later developing otocyst is reduced in size, which impacts gene expression. In contrast, the size of the otocyst in gain-of-function assays is not changed but the expression domains of several otocyst genes are reduced. Together these findings establish an interaction between Pa2G4 and Six1, and demonstrate that it has an important role in the development of tissues affected in BOS. Thereby, we suggest that pa2g4 is a potential candidate gene for BOS.

show abstract

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Pa2G4 is a novel Six1 co-factor that is required for neural crest and otic development

Neilson

Abbruzzesse

Kenyon

et al. 2017

Developmental Biology

View full text Add to dashboard Cite

show abstract

“…Another study proved that p48 EBP1 could repress the activity of p53 in brain tumors, suggesting that EBP1 may elicit oncogenic property in some types of cancers [21]. In particular, EBP1 knockout mice exhibit significant growth retardation, despite the fact that the mice have higher expression of Cyclin D1 than wild-type littermates [22]. These findings implicate that EBP1 may exert a complex role in the regulation of cell growth.…”

Section: Introductionmentioning

confidence: 94%

The downregulation of ErbB3 binding protein 1 (EBP1) is associated with poor prognosis and enhanced cell proliferation in hepatocellular carcinoma

Xiong

et al. 2014

Mol Cell Biochem

View full text Add to dashboard Cite

ErbB3 binding protein 1 (EBP1) has been recently reported to function as a tumor suppressor in the progression of multiple cancers, including breast cancer, prostate cancer, salivary adenoid cystic carcinoma (ACC), and oral squamous cell carcinoma (OSCC). However, the expression and physiological significance of EBP1 in hepatocellular carcinoma (HCC) remain unclear. In the study, we showed that EBP1 was significantly downregulated in clinical HCC specimens, and that decreased expression of EBP1 was associated with enhanced proliferation in HCC cells. Western blot and immunohistochemical analyses revealed that EBP1 was remarkably downregulated in HCC tissues compared with the adjacent normal ones. The levels of EBP1 were significantly associated with histological grade (P = 0.034), tumor size (P = 0.001), and Ki67 expression (P < 0.001) in HCC specimens. Univariate and multivariate analyses showed that EBP1 could serve as an independent prognostic indicator of patients' survival. Serum starvation and refeeding assay indicated that EBP1 was accumulated in growth-arrested HCC cells, and was progressively decreased when cells entered into S phase. Moreover, the depletion of EBP1 induced growth acceleration and cell cycle progression in L02 hepatocytes. On the basis of these findings, we conclude that EBP1 may be a valuable prognostic marker and promising therapeutic target of HCC.

show abstract

“…to Ebp1 WT cells, 35 it is apparent that the absence of Ebp1 expression is associated with elevated p53 protein levels and decreased expression of PCNA at both the mRNA and protein levels ( Figure 4B, left and middle). Overexpression of Ebp1 in MEF Ebp1 2/2 cells, which grow more poorly than their WT counterparts ( Figure 4B, right), enhances both PCNA expression and cell proliferation (supplemental Figure 4B).…”

Section: Gtp Binding Is Required For Tif-ia Regulation Of Rrna Synthesismentioning

confidence: 99%

Regulation of ribosomal RNA synthesis in T cells: requirement for GTP and Ebp1

Nguyen¹,

Lee

Urbani³

et al. 2015

Blood

View full text Add to dashboard Cite

show abstract

Alterations in cell growth and signaling in ErbB3 binding protein-1 (Ebp1) deficient mice

Cited by 43 publications

References 40 publications

Pa2G4 is a novel Six1 co-factor that is required for neural crest and otic development

Pa2G4 is a novel Six1 co-factor that is required for neural crest and otic development

The downregulation of ErbB3 binding protein 1 (EBP1) is associated with poor prognosis and enhanced cell proliferation in hepatocellular carcinoma

Regulation of ribosomal RNA synthesis in T cells: requirement for GTP and Ebp1

Contact Info

Product

Resources

About