This report describes an 8-year-old, 2-month-pregnant, Thoroughbred mare that was admitted with a week history of pyrexia, decreased appetite, respiratory distress, lethargy and recurrent fits of colic. The referring veterinarian had administered electrolyte solution and glucose, anti-inflammatory, antibiotic and analgesics; but this treatment had resulted in no improvement. The mare was admitted with a history of fever, colic, weight loss, inappetence, and signs of depression. Ultrasonographic examination was performed on both sides of the abdomen using a real-time scanner equipped with a 3.5 and/or 5.0 MHz convex transducer but no mass was imaged as there was relatively long distance between the mass and the anus. After sedation with xylazine and infiltration of 10mL of procaine Hcl, sampling of the abdominal lesion was carried out by the use of a 14-gauge spinal biopsy needle. Ten mL of the mass contents were collected and then submitted immediately for bacteriologic culture and antibiotic sensitivity. On day 7, percutaneous ultrasound-guided aspiration of the mass was performed for cavity evacuation under sedation with xylazine 2%. The area between the 9 th and the 17 th intercostal spaces was clipped, shaved and aseptically prepared. With ultrasonography, the best site for aspiration was determined to be the 14 th intercostal space where 10mL of procaine HCl was infiltrated locally. The aspiration location was selected so that the needle was advanced through the thickest portion of the mass. With ultrasound guidance, a 14G×170mm spinal needle was inserted into the abdominal mass using a free-hand technique. When the needle was advanced to the correct depth, the inner needle was removed, and a 50-mL syringe attached and pus was gradually aspirated. In conclusion, in the present report, results of physical and laboratory examination were not helpful. Sonography accompanied with fine-needle aspiration yielded a diagnosis and assisted in suspecting the prognosis of the case. To the best of the authors' knowledge, this case represents the 1 st confirmed report of successful treated abdominal abscess in the horse caused by Pseudomonas aeruginosa. Early sonographic location of abdominal abscess, accompanied with transcutaneous aspiration of the lesion and ultrasonographic follow-up monitoring is therefore recommended in horses with such presentations.