Alteration of tyrosine isoaccepting transfer ribonucleic acid species in wild-type and asporogenous strains of Bacillus subtilis

MATERIALS AND METHODS Strains. B. subtilis W168, B. subtilis W23 D obtained from R. H. Doi, and W23 S obtained from P. Schaeffer were stored as spore suspensions. Growth conditions. Cells were cultivated at 37 C with vigorous agitation on different media: nutrient broth medium (28); Penassay broth (Difco); SCM medium (11) (synthetic medium supplemented with Casamino Acids lDifco]); MS medium (29) (synthetic medium with 0.2% NH4Cl as a nitrogen source and 0.5% malate as a carbon source). MSS medium (8), synthetic sporulation medium supplemented with or without iron, contains (in grams per liter): sodium citrate, 2.0; sodium acetate, 2.0; glutamic acid, 2.0; NH4C1, 0.5; glycerol, 0.5. Iron was extracted from a 5 x-concentrated stock solution with chloroform-8-hydroxyquinoline by the method of Waring and Werkman (35). Before use, MnCl2 and (NO3)2Ca, filter sterilized, were added from stock solutions to give final concentrations of 10 ,uM and 0.5 mM, respectively. This medium was supplemented with or without 1 ml of 1 mM SO4Fe per liter, filter sterilized. Experimental cultures were inoculated from growing precultures in the same medium, themselves already having been inoculated with cells from overnight cultures at 30C in agar-nutrient broth medium.

Section: Fraction Numbermentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Study of tyrosine transfer ribonucleic acid modification in relation to sporulation in Bacillus subtilis

Menichi¹,

Heyman²

1976

“…Bacillus subtilis tRNATcan be separated by chromatography into two major species. One of these (tRNATyI) is predominant in vegetative cells, whereas the other (tRNATYIII) is the major component in stationary-phase cells and spores (1,10,12,18). tRNAT-" modification begins during the last division of exponential growth phase and is over by 2 h after the end of this phase.…”

mentioning

confidence: 99%

Thiomethylation of tyrosine transfer ribonucleic acid is associated with initiation of sporulation in Bacillus subtilis: effect of phosphate concentration

Buu¹,

Menichi²,

Heyman³

1981

The thiomethylation of Bacillus subtilis tyrosine transfer ribonucleic acid (tRNATyr) (i6A) has been shown to occur during the slowing-down of growth. The extent of this modification in stationary-phase cells grown in defined medium has been determined in parallel with the sporulation frequency. We observed that the presence of phosphate repressed sporulation and also inhibited the thiomethylation of tRNATyr (i6A) of B. subtilis W168. These effects were partially eliminated by decreasing the glucose concentration until it was growth limiting. In the case of strain W23S, in which sporulation is insensitive to glucose repression, sporulation and tRNATyr thiomethylation were not inhibited by nonlimiting concentrations of phosphate. These results suggest that both sporulation and tRNATyr hyper-modification share some common regulatory process.

“…Investigations of mechanisms controlling bacterial spore development have involved an intense examination of the regulatory role that tRNA may play in this system (18,20,21,35). Sporulation in Bacillus subtilis is accompanied by significant qualitative and quantitative changes in at least eight iso-accepting tRNA species (37).…”

mentioning

confidence: 99%

Transfer ribonucleic acid synthesis during sporulation and spore outgrowth in Bacillus subtilis studied by two-dimensional polyacrylamide gel electrophoresis

Henner¹,

Steinberg²

1979

The synthesis of transfer ribonucleic acid (tRNA) was examined during spore formation and spore outgrowth in Bacillus subtilis by two-dimensional polyacrylamide gel electrophoresis of in vivo 32P-labeled RNA. The two-dimensional gel system separated the B. subtilis tRNA's into 32 well-resolved spots, with the relative abundances ranging from 0.9 to 17% of the total. There were several spots (five to six) resolved which were not quantitated due to their low abundance. All of the tRNA species resolved by this gel system were synthesized at every stage examined, including vegetative growth, different stages of sporulation, and different stages of outgrowth. Quantitation of the separated tRNA's showed that in general the tRNA species were present in approximately the same relative abundances at the different developmental periods. tRNA turnover and comt Present address: