Alteration of the shoot radial pattern in Arabidopsis thaliana by a gain-of-function allele of the class III HD-Zip gene INCURVATA4

Ochando, Isabel; González-Reig, Santiago; Ripoll, Juan-José; Vera, Antonio; Martı́nez-Laborda, Antonio

doi:10.1387/ijdb.072306io

Cited by 27 publications

(27 citation statements)

References 48 publications

(64 reference statements)

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“…S5). In the presence of β-estradiol, XVE::ATHB8d plants display upward leaf curling (data not shown), as observed with miR 165/166-resistant variants of other HD-Zip III genes (Juarez et al, 2004;Mallory et al, 2004;Ochando et al, 2006;Ochando et al, 2008). ATHB2 mRNA levels were reduced in rev-5 and phv-11 phb-13 and increased after 4 hours of β-estradiol-induced ATHB8d expression (Fig.…”

Section: Athb2 Is a Direct Target Of Revsupporting

confidence: 72%

“…The abaxial marker YAB3 (Kumaran et al, 2002) is uniformly expressed throughout leaf primordia of hat3 athb4, thus demonstrating that simultaneous lack of HAT3 and ATHB4 is associated with loss of adaxial identity. The ectopic expression of HAT3, ATHB4 or ATHB2 results in upward leaf curling, a phenotype similar to that seen when HD-Zip III genes are expressed in both adaxial and abaxial regions of lateral organs (Juarez et al, 2004;Mallory et al, 2004;Ochando et al, 2006;Ochando et al, 2008). ATHB2:GUS was induced in the HAT3-ATHB4 domain on the adaxial side of leaf primordia of hat3 athb4, thus leading to an attenuation of the phenotype.…”

Section: Discussionmentioning

confidence: 76%

“…S5), indicating that ATHB2 expression is largely regulated at the transcriptional and/or post-transcriptional level. We then investigated whether ectopic expression of ATHB2 would result in upward leaf curling, which is usually observed when HD-Zip III genes are resistant to regulation by miRNAs and thus expressed in both adaxial and abaxial regions of lateral organs (Juarez et al, 2004;Mallory et al, 2004;Ochando et al, 2006;Ochando et al, 2008). Remarkably, 35S::3HA:ATHB2 leaves are severely curled upwards ( Fig.…”

Section: Research Articlementioning

confidence: 99%

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Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function

et al. 2013

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SUMMARYThe Arabidopsis genome encodes ten Homeodomain-Leucine zipper (HD-Zip) II proteins. ARABIDOPSIS THALIANA HOMEOBOX 2 (ATHB2), HOMEOBOX ARABIDOPSIS THALIANA 1 (HAT1), HAT2, HAT3 and ATHB4 are regulated by changes in the red/far red light ratio that induce shade avoidance in most of the angiosperms. Here, we show that progressive loss of HAT3, ATHB4 and ATHB2 activity causes developmental defects from embryogenesis onwards in white light. Cotyledon development and number are altered in hat3 athb4 embryos, and these defects correlate with changes in auxin distribution and response. athb2 gain-of-function mutation and ATHB2 expression driven by its promoter in hat3 athb4 result in significant attenuation of phenotypes, thus demonstrating that ATHB2 is functionally redundant to HAT3 and ATHB4. In analogy to loss-of-function mutations in HD-Zip III genes, loss of HAT3 and ATHB4 results in organ polarity defects, whereas triple hat3 athb4 athb2 mutants develop one or two radialized cotyledons and lack an active shoot apical meristem (SAM). Consistent with overlapping expression pattern of HD-Zip II and HD-Zip III gene family members, bilateral symmetry and SAM defects are enhanced when hat3 athb4 is combined with mutations in PHABULOSA (PHB), PHAVOLUTA (PHV) or REVOLUTA (REV). Finally, we show that ATHB2 is part of a complex regulatory circuit directly involving both HD-Zip II and HD-Zip III proteins. Taken together, our study provides evidence that a genetic system consisting of HD-Zip II and HDZip III genes cooperates in establishing bilateral symmetry and patterning along the adaxial-abaxial axis in the embryo as well as in controlling SAM activity.

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Section: Athb2 Is a Direct Target Of Revsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 76%

Section: Research Articlementioning

confidence: 99%

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Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function

et al. 2013

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“…17,20,22,33 The common phenotype of these dominant mutants is the development of radialized amphivasal vascular bundles, despite their phenotypic difference in meristem patterning and lateral organ development. In contrast, a point mutation in the START domain in AtHB15/INCURVATA4/CORONA resulted in a higher expression of the AtHB15/ICU4 transcripts and incurvature in leaves, but vascular patterning (bilateral organization) stays normal, 18,19 indicating that the function of AtHB15/ICU4/CNA is different from PHB, PHV and REV in vascular development. Taken together, interruption of the miR165/166 binding to the complementary sequence in HD-ZIP III TFs may affect vascular development.…”

Section: The Functions Of Hd-zip III Tfs In Vascular Differentiation mentioning

confidence: 92%

“…The HD-ZIP III TFs regulate a number of developmental processes, such as embryo patterning, meristem initiation and homeostasis, lateral organ polarity and vascular development in Arabidopsis. [11][12][13][16][17][18][19] In this review, we mainly focus on the functions of HD-ZIP III TFs in vascular differentiation and secondary cell wall development.…”

Section: The Functions Of Hd-zip III Tfs In Vascular Differentiation mentioning

confidence: 99%

The role of HD-ZIP III transcription factors andmiR165/166in vascular development and secondary cell wall formation

Wang

2015

Plant Signaling & Behavior

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Gene expression analysis in microdissected shoot meristems of Brassica napus microspore-derived embryos with altered SHOOTMERISTEMLESS levels

Elhiti

Wally

Belmonte

et al. 2012

Planta

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Altered expression of Brassica napus (Bn) SHOOTMERISTEMLESS (STM) affects the morphology and behaviour of microspore-derived embryos (MDEs). While down-regulation of BnSTM repressed the formation of the shoot meristem (SAM) and reduced the number of Brassica MDEs able to regenerate viable plants at germination, over-expression of BnSTM enhanced the structure of the SAM and improved regeneration frequency. Within dissected SAMs, the induction of BnSTM up-regulated the expression of many transcription factors (TFs) some of which directly involved in the formation of the meristem, i.e. CUP-SHAPED COTYLEDON1 and WUSCHEL, and regulatory components of the antioxidant response, hormone signalling, and cell wall synthesis and modification. Opposite expression patterns for some of these genes were observed in the SAMs of MDEs down-regulating BnSTM. Altered expression of BnSTM affected transcription of cell wall and lignin biosynthetic genes. The expression of PHENYLALANINE AMMONIA LYASE2, CINNAMATE 4-4HYDROXYLASE, and CINNAMYL ALCOHOL DEHYDROGENASE were repressed in SAMs over-expressing BnSTM. Since lignin formation is a feature of irreversible cell differentiation, these results suggest that one way in which BnSTM promotes indeterminate cell fate may be by preventing the expression of components of biochemical pathways involved in the accumulation of lignin in the meristematic cells. Overall, these studies provide evidence for a novel function of BnSTM in enhancing the quality of in vitro produced meristems, and propose that this gene can be used as a potential target to improve regeneration of cultured embryos.

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Alteration of the shoot radial pattern in Arabidopsis thaliana by a gain-of-function allele of the class III HD-Zip gene INCURVATA4

Cited by 27 publications

References 48 publications

Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function

Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function

The role of HD-ZIP III transcription factors andmiR165/166in vascular development and secondary cell wall formation

Gene expression analysis in microdissected shoot meristems of Brassica napus microspore-derived embryos with altered SHOOTMERISTEMLESS levels

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