Alteration of the lipid phase transition during mouse embryos freezing after in vitro culture with linoleic acid

Igonina, T. N.; Okotrub, Konstantin A.; Brusentsev, E. Yu.; Chuyko, E. A.; Ragaeva, D. S.; Ranneva, S.V.; Amstislavsky, S. Ya.

doi:10.1016/j.cryobiol.2021.01.014

Cited by 12 publications

(1 citation statement)

References 53 publications

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“…Cryopreservation was performed using a CL‐8800i programmable controlled rate freezer (CryoLogic, Australia) as described earlier (Igonina et al., 2021). Embryos were equilibrated with a cryoprotectant solution (CPS): FertiCult Flushing medium (FertiPro, Belgium) mixed with 1.8 M ethylene glycol (KhimMed, Russia) and 0.1 M sucrose as cryoprotectant agents (CPA), supplemented with 10% fetal calf serum.…”

Section: Methodsmentioning

confidence: 99%

Effect of cryopreservation on Odc1 and RhoA genes expression in diapausing mouse blastocysts

Amstislavsky,

Brusentsev,

Lebedeva

et al. 2024

Reprod Domestic Animals

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The possibility of embryo cryopreservation is important for applying the genome resource banking (GRB) concept to those mammalian species that exhibit embryonal diapause in their early development. Odc1 encodes ODC1, which is a key enzyme in polyamine synthesis. RhoA is an essential part of Rho/ROCK system. Both Odc1 and RhoA play an important role in preimplantation embryo development. Studying these systems in mammalian species with obligate or experimentally designed embryonic diapause may provide insight into the molecular machinery underlying embryo dormancy and re‐activation. The effect of cryopreservation procedures on the expression of the Odc1 and RhoA in diapausing embryos has not been properly studied yet. The purpose of this work is to address the possibility of cryopreservation diapausing embryos and to estimate the expression of the Odc1 and RhoA genes in diapausing and non‐diapausing embryos before and after freeze–thaw procedures using ovariectomized progesterone treated mice as a model. Both diapausing and non‐diapausing in vivo‐derived embryos continued their development in vitro after freezing–thawing as evidenced by blastocoel re‐expansion. Although cryopreservation dramatically decreased the expression of the Odc1 and RhoA genes in non‐diapausing embryos, no such effects have been observed in diapausing embryos where these genes were already at the low level before freeze–thaw procedures. Future studies may attempt to facilitate the re‐activation of diapausing embryos, for example frozen–thawed ones, specifically targeting Odc1 or Rho/ROCK system.

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Section: Methodsmentioning

confidence: 99%