Alteration of the Intestinal Intraepithelial Lymphocytes during Total Parenteral Nutrition

Kırıştıoğlu, İrfan; Teitelbaum, Daniel H.

doi:10.1006/jsre.1998.5408

Cited by 54 publications

(83 citation statements)

References 18 publications

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“…It has been previously shown that administration of TPN can lead to a number of alterations within the immune system of the small bowel mucosa. Among these changes is an increased expression of IFN-␥ and a loss of cells in the gutassociated lymphoid tissue (22,23,45). IELs eliminate infected and senescent cells through a combination of cytotoxicity, and via IFN-␥ and TNF-␣ release (46).…”

Section: Discussionmentioning

confidence: 99%

“…Administration of TPN was performed as previously described (22). Mice were infused with a crystalloid solution (5% dextrose in 0.45 normal saline with 20 mEq KCl/liter) at 4 ml/day.…”

Section: Operative Proceduresmentioning

confidence: 99%

“…The TPN groups received an i.v. TPN solution at 7 ml/24 h. The TPN solution has been described in detail previously (22), and contained a balanced mixture of amino acids, lipids, and dextrose in addition to electrolytes, trace elements, and vitamins. Caloric delivery was based on estimates of caloric intake by the control group and from previous investigators (23), so that caloric delivery was essentially the same in both groups.…”

Section: Operative Proceduresmentioning

confidence: 99%

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Intestinal Intraepithelial Lymphocyte γδ-T Cell-Derived Keratinocyte Growth Factor Modulates Epithelial Growth in the Mouse

Yang

Antony

Wildhaber

et al. 2004

The Journal of Immunology

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115

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Keratinocyte growth factor (KGF) promotes intestinal epithelial growth. To understand the relevance of intraepithelial lymphocyte (IEL)-derived KGF expression on epithelial growth, we used a mouse model of villus atrophy by the administration of total parenteral nutrition, and a model of villus hypertrophy by the creation of a short bowel syndrome. KGF expression was confined to γδ-ΤCR+ IELs. IEL-derived KGF expression was highest in the crypts, somewhat less in the lower portion of villi, and markedly lower in the upper portion of villi. Total parenteral nutrition administration was associated with a down-regulation of IEL-derived KGF expression, and short bowel syndrome was associated with an up-regulation of IEL-derived KGF expression. In the absence of γδ-ΤCR+ IEL, using γδ−/− mice, intestinal epithelial cell proliferation decreased in control, and in both mucosal atrophy (22% decline) and mucosal hypertrophy (14%) models. These results show that KGF from IELs is an important factor for maintenance of intestinal epithelial cell proliferation and villus growth.

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Section: Discussionmentioning

confidence: 99%

“…Administration of TPN was performed as previously described (22). Mice were infused with a crystalloid solution (5% dextrose in 0.45 normal saline with 20 mEq KCl/liter) at 4 ml/day.…”

Section: Operative Proceduresmentioning

confidence: 99%

Section: Operative Proceduresmentioning

confidence: 99%

See 1 more Smart Citation

Intestinal Intraepithelial Lymphocyte γδ-T Cell-Derived Keratinocyte Growth Factor Modulates Epithelial Growth in the Mouse

Yang

Antony

Wildhaber

et al. 2004

The Journal of Immunology

Self Cite

115

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“…The TPN ϩ EGF group received EGF at 25 g/kg per 12 h (in 200 l saline; Sigma-Aldrich, St. Louis, MO) via oral gavage, starting 1 day postcannulation (18). ErbB1 inhibitor, gefitinib (2.5 mg/ml in 1% aqueous Tween-80, 200 l; LC Laboratories, Woburn, MA) was administered via oral gavage twice daily, starting 3 days before intravenous cannulation and continuing until mice were killed (12,17,25). Nitrogen and energy delivery was matched between groups (isonitrogenous/isocaloric) (38); mice were killed 7 days postcannulation using CO 2.…”

Section: Methodsmentioning

confidence: 99%

Epidermal growth factor/TNF-α transactivation modulates epithelial cell proliferation and apoptosis in a mouse model of parenteral nutrition

Yang

Teitelbaum

2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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107

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Feng Y, Teitelbaum DH. Epidermal growth factor/TNF-␣ transactivation modulates epithelial cell proliferation and apoptosis in a mouse model of parenteral nutrition. Am J Physiol Gastrointest Liver Physiol 302: G236 -G249, 2012. First published November 10, 2011; doi:10.1152/ajpgi.00142.2011.-Epidermal growth factor (EGF) and tumor necrosis factor-␣ (TNF-␣) signaling are critical for effective proliferative and apoptotic actions; however, little is known about the codependency of these signaling pathways in the intestinal epithelium. Because total parenteral nutrition (TPN) is associated with loss of intestinal epithelial cell (IEC) proliferation and increased apoptosis, we utilized a mouse model to explore these transactivation pathways in small bowel epithelium. Mice underwent intravenous cannulation and were given enteral nutrition or TPN for 7 days. Outcomes included IEC proliferation, apoptosis, and survival. To address transactivation or dependence of EGF and TNF on IEC physiology, TNF-␣ receptor knockout (KO) mice, TNFR1-KO, R2-KO, or R1R2-double KO, were used. Exogenous EGF and pharmacological blockade of ErbB1 were performed in other groups to examine the relevance of the ErB1 pathway. TPN increased IEC TNFR1 and decreased EGF and ErbB1 abundance. Loss of IEC proliferation was prevented by exogenous EGF or blockade of TNFR1. However, EGF action was prevented without effective TNFR2 signaling. Also, blockade of TNFR1 could not prevent loss of IEC proliferation without effective ErbB1 signaling. TPN increased IEC apoptosis and was due to increased TNFR1 signaling. Exogenous EGF or blockade of TNFR1 could prevent increased apoptosis, and both pathways were dependent on effective ErbB1 signaling. Exogenous EGF prevented increased apoptosis in mice lacking TNFR2 signaling. TPN mice had significantly decreased survival vs. controls, and this was associated with the TNFR1 signaling pathway. We concluded that these findings identify critical mechanisms that contribute to TPN-associated mucosal atrophy via altered TNF-␣/EGF signaling. It emphasizes the importance of both TNFR1 and TNFR2 pathways, as well as the strong interdependence on an intact EGF/ErbB1 pathway.

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“…EC mRNA (poly-A positive) was reversed transcribed into cDNA following a standard protocol (19). Specific primers for selected gene sequences of the members of the intrinsic (bcl-2, bax) and extrinsic (TNF-α, Fas) apoptotic pathways were designed using proprietary software (LaserGene, DNAStar, Inc, Madison, WI).…”

Section: Mucosal Cell Isolation and Purification-mucosal Cells Were Imentioning

confidence: 99%

Influence of the site of small bowel resection on intestinal epithelial cell apoptosis

et al. 2005

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Alteration of the Intestinal Intraepithelial Lymphocytes during Total Parenteral Nutrition

Cited by 54 publications

References 18 publications

Intestinal Intraepithelial Lymphocyte γδ-T Cell-Derived Keratinocyte Growth Factor Modulates Epithelial Growth in the Mouse

Intestinal Intraepithelial Lymphocyte γδ-T Cell-Derived Keratinocyte Growth Factor Modulates Epithelial Growth in the Mouse

Epidermal growth factor/TNF-α transactivation modulates epithelial cell proliferation and apoptosis in a mouse model of parenteral nutrition

Influence of the site of small bowel resection on intestinal epithelial cell apoptosis

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