Alteration of the fatty acid composition of Brassica napus L. via overexpression of phospholipid: Diacylglycerol acyltransferase 1 from Sapium sebiferum (L.) Roxb.

Zhou, Bo; Fei, Wenjie; Yang, Shiquan; Yang, Feng Ling; Qu, Gaoyi; Tang, Weiwei; Ou, Jianping; Peng, Dan

doi:10.1016/j.plantsci.2020.110562

Cited by 6 publications

(4 citation statements)

References 38 publications

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“…S. sebiferum oil has been proposed as a potential substitute for biodiesel production. Indeed, its outstanding flash point, low cold flow value, and satisfactory oxidation stability satisfy the EN 14214 and ASTM 6751–03 standards [8] .…”

Section: Introductionmentioning

confidence: 89%

“…Sapium sebiferum (in the Euphorbiaceae family), also known as the Chinese tallow tree, is distributed worldwide [6] . As one of the four woody oil crops, S. sebiferum has been cultivated as an economic crop for approximately 1,400 years in China [7] , [8] . Its seeds possess more oil than cottonseed, flaxseed, and soybeans, as each seed comprises approximately 50 % oil, with the proportion of unsaturated fatty acids reaching up to 90 % [9] , [10] .…”

Section: Introductionmentioning

confidence: 99%

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Application of an aqueous enzymatic–ultrasound cavitation method for the separation of Sapium sebiferum seed kernel oil

Liu,

Liao,

Wei

et al. 2023

Ultrasonics Sonochemistry

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Section: Introductionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Application of an aqueous enzymatic–ultrasound cavitation method for the separation of Sapium sebiferum seed kernel oil

Liu,

Liao,

Wei

et al. 2023

Ultrasonics Sonochemistry

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“…The pull strategy aims to ensure efficient assembly of FAs generated in the plastid into TG at the ER ( Figure 2 ). Most of the metabolic engineering studies that attempted to optimize this step in seeds have focused on the over-expression of diacylglycerol acyltransferase (DGAT) ( He et al., 2004 ; Kroon et al., 2006 ; Lung and Weselake, 2006 ; Shockey et al., 2006 ; Yu et al., 2006 ; Burgal et al., 2008 ; Misra et al., 2013 ; Wang et al., 2014 ; Liu et al., 2015 ; Savadi et al., 2015 ; Zhou et al., 2020 ; Chen et al., 2022 ) and phospholipid: diacylglycerol acyltransferase 1 (PDAT1) ( Zhang et al., 2010 ; van Erp et al., 2011 ; Guan et al., 2015 ; Zhou et al., 2020 ) which commit acyl chains to storage TGs. Manipulation of other genes encoding for “pull” proteins, such as glycerol-3-phosphate dehydrogenases (GPDH), glycerol-3-phosphate acyltransferase (GPAT), and lysophosphatidic acid acyltransferase (LPAT), have also been reported to increase TG levels in seeds ( Liu et al., 2015 ) ( Table 5 ).…”

Section: Metabolic Engineering Strategies To Improve Seed Oil Content...mentioning

confidence: 99%

Progress in understanding and improving oil content and quality in seeds

2023

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The world’s population is projected to increase by two billion by 2050, resulting in food and energy insecurity. Oilseed crops have been identified as key to address these challenges: they produce and store lipids in the seeds as triacylglycerols that can serve as a source of food/feed, renewable fuels, and other industrially-relevant chemicals. Therefore, improving seed oil content and composition has generated immense interest. Research efforts aiming to unravel the regulatory pathways involved in fatty acid synthesis and to identify targets for metabolic engineering have made tremendous progress. This review provides a summary of the current knowledge of oil metabolism and discusses how photochemical activity and unconventional pathways can contribute to high carbon conversion efficiency in seeds. It also highlights the importance of 13C-metabolic flux analysis as a tool to gain insights on the pathways that regulate oil biosynthesis in seeds. Finally, a list of key genes and regulators that have been recently targeted to enhance seed oil production are reviewed and additional possible targets in the metabolic pathways are proposed to achieve desirable oil content and quality.

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“…PDAT was also reported to have overlapping functions with DGAT for TAG synthesis in Arabidopsis [ 74 ]. PDAT overexpression increased 29–47% of total fatty acids [ 73 ] and rescued oleic acid sensitivity and TAG accumulation in yeast [ 75 ], which was also applied to a recent study expressing PDAT from Sapium sebiferum (L.) Roxb [ 76 ].…”

Section: Modular Metabolic Engineering Strategies For Standard and Hi...mentioning

confidence: 99%

Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in Yeast Saccharomyces cerevisiae

Jiang

et al. 2022

JoF

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Microbial lipids have been a hot topic in the field of metabolic engineering and synthetic biology due to their increased market and important applications in biofuels, oleochemicals, cosmetics, etc. This review first compares the popular hosts for lipid production and explains the four modules for lipid synthesis in yeast, including the fatty acid biosynthesis module, lipid accumulation module, lipid sequestration module, and fatty acid modification module. This is followed by a summary of metabolic engineering strategies that could be used for enhancing each module for lipid production. In addition, the efforts being invested in improving the production of value-added fatty acids in engineered yeast, such as cyclopropane fatty acid, ricinoleic acid, gamma linoleic acid, EPA, and DHA, are included. A discussion is further made on the potential relationships between lipid pathway engineering and consequential changes in cellular physiological properties, such as cell membrane integrity, intracellular reactive oxygen species level, and mitochondrial membrane potential. Finally, with the rapid development of synthetic biology tools, such as CRISPR genome editing tools and machine learning models, this review proposes some future trends that could be employed to engineer yeast with enhanced intracellular lipid production while not compromising much of its cellular health.

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Alteration of the fatty acid composition of Brassica napus L. via overexpression of phospholipid: Diacylglycerol acyltransferase 1 from Sapium sebiferum (L.) Roxb.

Cited by 6 publications

References 38 publications

Application of an aqueous enzymatic–ultrasound cavitation method for the separation of Sapium sebiferum seed kernel oil

Application of an aqueous enzymatic–ultrasound cavitation method for the separation of Sapium sebiferum seed kernel oil

Progress in understanding and improving oil content and quality in seeds

Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in Yeast Saccharomyces cerevisiae

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