Alteration of structure and penetrability of the vitelline envelope after passage of eggs from coelom to oviduct in <i>Xenopus laevis</i>

Grey, Robert D.; Hedrick, Jerry L.

doi:10.1002/jez.1402010109

Cited by 70 publications

(48 citation statements)

References 22 publications

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“…Similar changes have been described for other Amphibian species (11,15,12). In fact, the action during only 30 min of "pars recta" secretions makes the vitelline coat of coelomic oocytes liable to spermatic lysines.…”

Section: Conditioned Coelomic Oocytessupporting

confidence: 75%

Morphological Modifications of Oocyte Vitelline Envelope from Bufo arenarum during Different Functional States

Mariano¹,

Martin²,

Pisanó³

1984

Dev Growth Differ

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The submicroscopic morphology of the vitelline envelope of Bufo arenarum's oocyte change significantly during the maturation and fertilization processes. The morphological changes are related to physiological activity in vivo and can be triggered in vitro by experimental procedures. It is our scope to present the ultrastructure differences of fascicular components of the vitelline envelope in coelomic, "pars recta" conditioned, oviductal, oviposited and fertilized oocytes. Our experimental results indicate that artificial "pars recta" treatment of coelomic oocytes arrange the fascicular components as those of oviposited oocyte, although differences still remain indicating that additional maturation processes take place while the egg pass througth the oviduct. Fertilized or activated oocytes which are refractary to sperm penetration, change the vitelline envelope fascicular components orientation giving a submicroscopical image quite different to those of none fertilized oocytes. These ultrastructural changes define in a clear cut manner the functional states of Bufo arenarum's oocyte.The Amphibian oocyte, due to its peculiar characteristics, has been used extensively not only in the field of descriptive developmental biology, but also in the molecular biology realm. Researches to know the sequence of events related with changes occurring in the gametes during fertilization, have been extensively performed recently. Many of them have been done to get acquaintance on the structure of the non cellular envelopes of the oocyte and their functions during that process; both the jelly as well as the vitelline envelope (V. E.) have been considered Different and extensive observations of the fertilization process in distinct animal species gave support to the hypothesis that the fertilization is dependent on the liability of the V. E. to the proteolytic enzymes present in the spermatozoa acrosome and to the mechanical penetration of the sperm themselves, with some differences according to the species (7-SO).Ultrastructural modifications of the V. E. of the coelomic oocytes of Amphibians are known to occur when the cell has moved one (cm) inside the oviduct; GREY et al.(1 1) have observed that the eggs of Xenopus laevis which entered the oviduct physiologically, have a modification of their vitelline envelope.

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Section: Conditioned Coelomic Oocytessupporting

confidence: 75%

Morphological Modifications of Oocyte Vitelline Envelope from Bufo arenarum during Different Functional States

Mariano¹,

Martin²,

Pisanó³

1984

Dev Growth Differ

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“…Although alterations in ZP thickness have been reported after ovulation [20], our results show that, if true, they do not affect the fasciculated configuration. However, changes in bundle association (compaction) were observed in the vitelline envelope of Xenopus, after ovulation [34], and modifications may also occur particularly in the outer layer. Differences in pig ZP between oocytes matured in vitro or in vivo may be attributed to an incomplete maturation in the first case and/ or to extracellular matrix addition in the second case [20,35].…”

Section: Texturementioning

confidence: 99%

Texture of the zona pellucida of the mature pig oocyte. The mammalian egg envelope revisited

et al. 2004

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-The zona pellucida (ZP) of mature pig oocytes is believed to consist of a dense filamentous meshwork, less compact on the inner and outer faces. The uneven surface of the ZP is made of unordered and stretched fibrils surrounding deep funnels which are the openings of the radial canaliculi. The topography of the ZP surface may contribute to the initial interplay between male and female gametes. Using cytochemical techniques for transmission electron microscopy (TEM), such as tannic acid and ruthenium red treatments, we found that the ZP of pig oocytes was essentially made of bundles of fibrils distributed in concentric layers (except in the innermost and outer parts). A correlation appears between the dense structure of the core layer of the ZP and its texture: it is constituted of superposed layers of fibril bundles, whereas only a random meshwork is found in a very thin innermost and in the outer layer. The fascicular configuration may control the permeability of the ZP, giving its semi-rigidity and elasticity, and may facilitate sperm penetration. The liquid crystal-like design of the core layer of the ZP is similar to textures found in the the vitelline envelope (zona radiata) of other vertebrates and possibly of all the deuterostomes. Such texture is probably related to the unique ZP protein composition and to a coordinated synthesis. vitelline envelope / ultrastructural cytochemistry / glycoproteins / liquid crystal-like texture / Sus scrofa Abbreviations PVS: perivitelline space; SEM: scanning electron microscopy; TEM: transmission electron microscopy; ZP: zona pellucida.

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“…Katagiri et a1 [22] have demonstrated that an extract of the pars recta region of the oviduct from Bufo Bufojuponicus can cause the morphological alteration of the CE to VE in Bufo eggs. In the eggs of Xenopus laevis, a morphological change of the CE to VE can be induced at pH 6 or 9 [2]. However, these nonphysiological conditions would not likely cause the 43K to 41K conversion demonstrated here, but could possibly Identification of the chemical nature of the processing event involved in the 43K to 41K conversion and isolation of the oviductal hydrolase catalyzing this processing event will be necessary in order to understand the ultrastructural and functional (sperm penetrability) differences in the CE and VE.…”

Section: Discussionmentioning

confidence: 99%

The coelomic envelope to vitelline envelope conversion in eggs of Xenopus laevis

Gerton

Hedrick

1986

J of Cellular Biochemistry

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An amphibian egg recovered from the body cavity is enclosed by a coelomic egg envelope. Upon transport down the oviduct, the envelope is converted to the vitelline envelope. The coelomic and vitelline envelopes are distinct in terms of sperm penetrability, ultrastructural morphology, and radioiodination profiles. In this study, the macromolecular compositions of these two envelopes were determined. Isolated envelopes were compared by one- and two-dimensional gel electrophoresis, peptide mapping, and radiolabeling. A protein with a molecular weight of 57,000 (57K) was present in the vitelline envelope but was absent in the coelomic envelope. A glycoprotein with a molecular weight of 43K in the coelomic envelope was converted to a component with a molecular weight of 41K in the vitelline envelope. The 43K-molecular weight component of the coelomic envelopes could be radioiodinated by lactoperoxidase but no labeling of the 41K-molecular weight component occurred in the vitelline envelope. Peptide mapping using limited proteolysis established that the 43K-molecular weight component of the coelomic envelope was a precursor to the 41K-molecular weight component of the vitelline envelope. These molecular alterations may underlie the ultrastructural and physiological changes occurring in these envelopes.

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Alteration of structure and penetrability of the vitelline envelope after passage of eggs from coelom to oviduct in Xenopus laevis

Cited by 70 publications

References 22 publications

Morphological Modifications of Oocyte Vitelline Envelope from Bufo arenarum during Different Functional States

Morphological Modifications of Oocyte Vitelline Envelope from Bufo arenarum during Different Functional States

Texture of the zona pellucida of the mature pig oocyte. The mammalian egg envelope revisited

The coelomic envelope to vitelline envelope conversion in eggs of Xenopus laevis

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