Alteration of proline hydroxylase activity by glucocorticoids

Cutroneo, Kenneth R.; Costello, Donald H.; Fuller, George C.

doi:10.1016/0006-2952(71)90190-0

Cited by 32 publications

(5 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A novel approach is described to regulate in vivo the expression of a specific gene by using a promoter competitor or adecoy® oligodeoxynucleotide to compete with the cis element in the natural gene for the transacting factor. SsPTs mimic the selective effect of glucocorticoids on collagen synthesis, 14,15 and exert antifibrotic activity like glucocorticoids 11,12,16 . The one overriding benefit of the PTs is that they are without the many untoward side‐effects of glucocorticoids and may be potentially useful to alter the development of fibrosis using targeted liposomes.…”

Section: Resultsmentioning

confidence: 99%

A sense phosphorothioate oligodeoxynucleotide containing the transforming growth factor beta regulatory element acts as a novel local nonsteroidal antifibrotic drug

Cutroneo

2000

Wound Repair Regeneration

View full text Add to dashboard Cite

Fibrosis is a potential response to tissue injury. At present, glucocorticoids with their numerous toxic side effects are the only effective treatment for fibrotic diseases. Granulomas induced by sponge implantation were treated with single-stranded phosphorothioate oligodeoxynucleotides containing the wild type or mutated transforming growth factor-beta response element designed to inhibit the rat proalpha1(I) promoter activity. Single-stranded phosphorothioate oligonucleotides resulted in antifibrotic activity based on their ability to reduce granuloma tissue formation and selectively inhibit collagen synthesis. The mutated single-standed phosphorothioate oligonucleotides or dexamethasone given at an equivalent dose to single-standed phosphorothioate oligonucleotides failed to do so. These data suggest that the phosphorothioate oligodeoxynucleotide containing the transforming growth factor-beta regulatory element has an antifibrotic effect and may be used to inhibit the development of fibrosis.

show abstract

Section: Resultsmentioning

confidence: 99%

A sense phosphorothioate oligodeoxynucleotide containing the transforming growth factor beta regulatory element acts as a novel local nonsteroidal antifibrotic drug

Cutroneo

2000

Wound Repair Regeneration

View full text Add to dashboard Cite

show abstract

“…Thus, the teratogenic effect of lathyrism in sheep due to lack of lysyl oxidase has been described (22). Furthermore, a teratogenic effect of cortisone administration in pregnant rats due to proline hydroxylase inhibition has been reported (13).…”

Section: Discussionmentioning

confidence: 99%

Changes in Conformation of Collagen During Development of Bovine Ruminal Mucosa^1,²

Arias

González

Vivanco

et al. 1983

Zentralblatt für Veterinärmedizin Reihe A

View full text Add to dashboard Cite

One of the most evident morphogenetic features occurring during ontogenesis of the ruminal mucosa is the development of the ruminal papillae.This morphological event starts in foetuses at 5 months of age, appearing as undulations involving the deeper layers of the epithelium, basement membrane and underlying lamina propria (2). The morphogenesis involves ultrastructural changes in the epithelial cells (3, 5) as well as changes in the distribution of the fibrillar and cellular components of the connective tissue (6). These latter consist mainly in an increase of the fibrillar collagen density (2).The morphogenetic role of collagen and its associated glycosaminoglycans (GAG) has been demonstrated in studies of tissues in which epithelial-mesenchymal interactions operated (10,17,20). For reviews on the role of collagen in morphogenesis see also SHOSHAN and GROSS (32), RAMACHANDRAN and REDDI (28) and BORSTEIN and SAGE (1 1).The exact mechanism of the tissue interactions which operate in morphogenesis and how they are affected by collagen and GAG are, as yet, far from being understood. This is especially evident in ruminal ontogenesis where relatively little is known about the mechanisms operating in papillary morphogenesis.The purpose of the present study was to investigate whether, during ruminal development, there are collagen changes associated with the morphological changes reported elsewhere (2, 6).A punctual study was made to characterize, using physicochemical criteria, the conformational changes occurring in the collagen of the foetal ruminal mucosa during development.' Thanks are due to Dr. A. HORVATH for his suggestions.

show abstract

“…In 1971, the effect of glucocorticoids on granuloma tissue prolyl hydroxylase was described. The effect was an inhibitory effect on either the amount or activity of this enzyme [Cutroneo et al, 1971]. This prolyl hydroxylase activity inhibition was later shown to be temporally related in pre‐existing granulomas as compared to other tissues [Counts and Cutroneo, 1979].…”

Section: How Do Glucocorticoids Inhibit Collagen Synthesis?mentioning

confidence: 99%

How do glucocorticoids compare to oligo decoys as inhibitors of collagen synthesis and potential toxicity of these therapeutics?

Cutroneo

Sterling

2004

J of Cellular Biochemistry

Self Cite

View full text Add to dashboard Cite

This article demonstrates how glucocorticoids decrease collagen synthesis. The parameters used to assess procollagen synthesis in our laboratory will be compared to those used by others. This article will note all the pertinent literature on the molecular mechanisms of this down regulation of procollagen synthesis. For example, what are the effects of glucocorticoids at the levels of transcription and translation of collagen mRNAs? Finally, we will define a molecular mechanism to inhibit Type I collagen synthesis by decreasing the binding of the TGF-beta activator protein complex to the TGF-beta element in the distal promoter of the proalpha1 Type I collagen gene, preventing the 2:1 ratio of alpha1 to alpha2 chains in the processed Type I collagen molecule. We will next ask "How do sense oligo decoys decrease Type I collagen synthesis at the in vivo and at the cell levels?" In primary fibrotic cell culture, the double-stranded phosphorothioate oligodeoxynucleotide decoys were more effective than their sense single-stranded counterparts. The molecular mechanism for the decrease in Type I collagen synthesis is the same as glucocorticoids, that is by decreasing the binding of the TGF-beta activator protein complex to the TGF-beta element in the distal promoter of the proalpha1 Type I collagen gene for the transcription of the proalpha1 mRNAs. The reason for using sense oligo decoys as anti-fibrotic agents as compared to the anti-fibrotic glucocorticoids, is that presently marketed and FDA approved glucocorticoids have many untoward side effects which the sense oligo decoys do not have.

show abstract

Alteration of proline hydroxylase activity by glucocorticoids

Cited by 32 publications

References 20 publications

A sense phosphorothioate oligodeoxynucleotide containing the transforming growth factor beta regulatory element acts as a novel local nonsteroidal antifibrotic drug

A sense phosphorothioate oligodeoxynucleotide containing the transforming growth factor beta regulatory element acts as a novel local nonsteroidal antifibrotic drug

Changes in Conformation of Collagen During Development of Bovine Ruminal Mucosa^1,²

How do glucocorticoids compare to oligo decoys as inhibitors of collagen synthesis and potential toxicity of these therapeutics?

Contact Info

Product

Resources

About

Alteration of proline hydroxylase activity by glucocorticoids

Cited by 32 publications

References 20 publications

A sense phosphorothioate oligodeoxynucleotide containing the transforming growth factor beta regulatory element acts as a novel local nonsteroidal antifibrotic drug

A sense phosphorothioate oligodeoxynucleotide containing the transforming growth factor beta regulatory element acts as a novel local nonsteroidal antifibrotic drug

Changes in Conformation of Collagen During Development of Bovine Ruminal Mucosa1,2

How do glucocorticoids compare to oligo decoys as inhibitors of collagen synthesis and potential toxicity of these therapeutics?

Contact Info

Product

Resources

About

Changes in Conformation of Collagen During Development of Bovine Ruminal Mucosa^1,²