Alteration of plasma membrane lipids in aluminum-resistant and aluminum-sensitive wheat genotypes in response to aluminum stress

Abstract

1997. Alteration of plasma membrane lipids in aluminum-resistant and aluminum-sensitive wheal genotypes in response to aluminum stress. -Physiol Plant. 99; 302-308.We have studied Ihe effect of aluminum (Al) on lipid composition of plasma membranes from roots of an Al-resistant(Pr741)and an Al-sensitive (Katepwa)cullivarof Triticum aestivum L. Several genotype-specific changes were observed in phospholipids and steryl lipids. While exposure to 20 \}M AlCl, for 3 days had no effect on total phospholipids in either genotype, the most abundant phospholipid, phosphatidyicholine. increased significantly in the .Al-resistant Pr741 with corresponding decreases in other phospholipids. In comparison, no change in phosphatidylcholine was observed in the Al-sensitive Katepwa. Aluminum also decreased steryl lipids (mainly free sterols) in PT741. Such changes were not observed in Katepwa. As a result of differential changes in lipid composition, the relative abundance of one iipid class to another changed. The ralio of steryl iipids to phospholipids decreased in Pr741. with no change in Katepwa. While limited information on the relationship between membrane function and lipid composition makes it difficult to relate these changes lo Al toxicily and resistance, changes observed only in Ihe A)-resistant genotype could contribute lo continued plant growth in the face of Al stress.

A Higher Plant Δ8 Sphingolipid Desaturase with a Preference for (Z)-Isomer Formation Confers Aluminum Tolerance to Yeast and Plants

“…Whereas it remains unclear which, if any, of these reactions are primary causes for aluminum toxicity in plants, it is plausible that aluminum-dependent changes in cell membrane structure and function contribute to the overall stress encountered in acid soils. Consistent with this idea are reports demonstrating that aluminum can reduce membrane fluidity of the Archaebacterium Thermoplasma acidophilum by binding to the polar head groups of phospholipids (Deleers et al, 1986) and alter the lipid composition of plant roots (Lindberg and Griffiths, 1993;Zhang et al, 1997;Peixoto et al, 2001;Stival da Silva et al, 2006). Previous reports have also shown that genetically engineered changes to the lipid composition of plant and yeast (Saccharomyces cerevisiae) membranes can affect their susceptibility to chilling, photoinhibition, drought, fungal toxins, and ion toxicity (Avery et al, 1996;Nishida and Murata, 1996;Delhaize et al, 1999;Thevissen et al, 2000;Los and Murata, 2004;Zhang et al, 2005; Stival da Silva et al, 2006).…”

Molecular Basis of Aluminium Toxicity in Plants: A Review

“…Although Al resistance has been a successful and active area of research; however, the underlying molecular, genetic and physiological principles are still not well understood. The cellular components and processes which have been proposed to be affected by Al are wide ranging and some of the most important include; cell nuclei, mitosis and cell division [147], composition, physical properties and structure of the plasma membrane [116,117], uptake of Ca and other ions [118,160], phosphoinositide-mediated signal transduction and cytoplasmic calcium homeostasis [20,52], oxidative stress [105], cytoskeletal dynamics [56] and the cell wall-plasma membrane-cytoskeleton continuum [61].…”

“…There are several recent reviews/researches that discuss mechanisms of Al tolerance and toxicity in plants by [7,9,15,16,[111][112][113][114][115]. Al toxicity affects a number of cellular components such as composition, physical properties and structure of the plasma membrane [116,117], cell nuclei, mitosis and cell division [Silva, et al, 2000], uptake of Ca 2+ and other ions [118] and cytoskeletal dynamics [56] and many more. Primary target of Al toxicity is the disturbance of cytoplasmic Ca 2+ -homeostasis [52] and may be involved in the inhibition of the cell division.…”

Characterization of Glucosylceramides in thePolygonaceae,Rumex obtusifoliusL. Injurious Weed

“…[8][9][10] Numerous reports have been published on the structure and composition of glucosylceramides in the plant kingdom, [11][12][13] although no study has previously been reported on the analysis of sphingolipids in Polygonaceae, including Rumex obtusifolius L. The subcellular localization of the glucosylceramide molecules is likely to provide insight into its function. Glucosylceramides are localized in the plasma membrane and tonoplast as a major lipid class.…”