Alteration of oral flora in betel quid chewers in Sri Lanka

Uehara, Osamu; Hiraki, Daichi; Kuramitsu, Yasuhiro; Matsuoka, Hirofumi; Takai, Rie; Fujita, Mari; Harada, Fumiya; Paudel, Durga; Takahashi, Shuhei; Yoshida, Koki; Muthumala, Malsantha; Nagayasu, Hiroki; Chiba, Itsuo

doi:10.1016/j.jmii.2020.06.009

Cited by 12 publications

(11 citation statements)

References 16 publications

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“…The disturbance of ocular flora may be another essential factor affecting the ocular comfort of OP patients. Existing studies have proved that changes in intestinal, oral, vaginal and skin microbial communities will significantly affect the homeostasis of the body ( Goldsmith and Sartor, 2014 ; Guenin-Macé et al., 2020 ; Oh et al., 2021 ; Uehara et al., 2021 ). Therefore, the investigation of ocular microbiota of patients with ocular prosthesis may provide a new method to improve ocular comfortableness.…”

Section: Discussionmentioning

confidence: 99%

Conjunctival sac microbiome in anophthalmic patients: Flora diversity and the impact of ocular prosthesis materials

Zhao

Chen

Zheng

et al. 2023

Front. Cell. Infect. Microbiol.

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PurposeTo explore the changes of bacterial flora in anophthalmic patients wearing ocular prosthesis (OP) and the microbiome diversity in conditions of different OP materials.MethodsA cross-sectional clinical study was conducted, involving 19 OP patients and 23 healthy subjects. Samples were collected from the upper, lower palpebral, caruncle, and fornix conjunctiva. 16S rRNA sequencing was applied to identify the bacterial flora in the samples. The eye comfort of each OP patient was determined by a questionnaire. In addition, demographics information of each participant was also collected.ResultsThe diversity and richness of ocular flora in OP patients were significantly higher than that in healthy subjects. The results of flora species analysis also indicated that in OP patients, pathogenic microorganisms such as Escherichia Shigella and Fusobacterium increased significantly, while the resident flora of Lactobacillus and Lactococcus decreased significantly. Within the self-comparison of OP patients, compared with Polymethyl Methacrylate (PMMA), prosthetic material of glass will lead to the increased colonization of opportunistic pathogens such as Alcaligenes, Dermabacter and Spirochaetes, while gender and age have no significant impact on ocular flora.ConclusionsThe ocular flora of OP patients was significantly different from that of healthy people. Abundant colonization of pathogenic microorganisms may have an important potential relationship with eye discomfort and eye diseases of OP patients. PMMA, as an artificial eye material, demonstrated potential advantages in reducing the colonization of opportunistic pathogens.

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Section: Discussionmentioning

confidence: 99%

Conjunctival sac microbiome in anophthalmic patients: Flora diversity and the impact of ocular prosthesis materials

Zhao

Chen

Zheng

et al. 2023

Front. Cell. Infect. Microbiol.

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“…infantis , a commensal oral microorganism [ 61 ] was found to increase considerably in BQC. Streptococcus salivarius has been shown to be significant in the cancer samples as compared to control samples [ 42 , 62 ] which can serve as a diagnostic marker along with Prevotella species for BQ association with OSCC [ 31 , 44 , 52 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…An increased abundance for Prevotella , Lactobacillus , Faecalibacterium , Ruminicoccus , and Bifidobacterium genera observed among the microbial community of BQC was in sync with the recent study by Uehara et. al, 2021 [ 44 ]. While, a decreasing pattern for Bacteroides , Streptococcus , Neisseria , Haemophilus , Fusobacterium , and Veillonella in BQC was observed ( S12 Fig ).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the present study aims to identify the taxonomic compositional changes in the oral microbiome through 16S rRNA sequencing and community structure variance in BQ chewers and non-chewers. This will facilitate the understanding of whether the chewing and associated substances will lead to dysbiosis which may further be associated with progression of oral diseases including cancer [ 1 , 43 , 44 ]. This approach may also lead to identification of potential strategies for early detection of precancerous changes and apply this information in developing consortia of microbes, which can reverse the changes in the oral cavity of an individual through probiotic-based products.…”

Section: Introductionmentioning

confidence: 99%

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Identifying oral microbiome alterations in adult betel quid chewing population of Delhi, India

et al. 2023

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The study targets to establish a factorial association of oral microbiome alterations (oral dysbiosis) with betel quid chewing habits through a comparison of the oral microbiome of Betel quid chewers and non-chewing individuals. Oral microbiome analysis of 22 adult individuals in the Delhi region of India through the 16S sequencing approach was carried out to observe the differences in taxonomic abundance and diversity. A significant difference in diversity and richness among Betel Quid Chewers (BQC) and Betel Quid Non-Chewers (BQNC) groups was observed. There were significant differences in alpha diversity among the BQC in comparison to BQNC. However, in the age group of 21–30 years old young BQC and BQNC there was no significant difference in alpha diversity. Similar result was obtained while comparing BQC and Smoker-alcoholic BQC. BQ smoker-chewers expressed significant variance in comparison to BQC, based on cluster pattern analysis. The OTU-based Venn Diagram Analysis revealed an altered microbiota, for BQ chewing group with 0–10 years exposure in comparison to those with 10 years and above. The change in the microbial niche in early chewers may be due to abrupt chemical component exposure affecting the oral cavity, and thereafter establishing a unique microenvironment in the long-term BQC. Linear discriminant analysis revealed, 55 significant features among BQC and Alcoholic-Smoker BQC; and 20 significant features among BQC and Smoker BQC respectively. The study shows the abundance of novel bacterial genera in the BQC oral cavity in addition to the commonly found ones. Since the oral microbiome plays a significant role in maintaining local homeostasis, investigating the link between its imbalance in such conditions that are known to have an association with oral diseases including cancers may lead to the identification of specific microbiome-based signatures for its early diagnosis.

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“…The dose-response relationship between betel chewing and increased risk for OPMD and oral cancer augmented by the duration of practice has also been established [19][20][21]. Moreover, this habit has been linked to an array of oral mucosal diseases ranging from chewer's mucosa to OPMD, high burden of periodontal disease and poor oral hygiene [22][23][24]. In addition, there is research evidence to suggest that the betel chewing was related to metabolic syndrome, the precursor condition for many non-communicable diseases [25,26].…”

Section: Introductionmentioning

confidence: 99%

The Role of Selected Habits, Periodontal Disease and Oral Hygiene Status on the Occurrence and Prognosis of Oral Mucosal Lesions

2021

AOD

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Alteration of oral flora in betel quid chewers in Sri Lanka

Cited by 12 publications

References 16 publications

Conjunctival sac microbiome in anophthalmic patients: Flora diversity and the impact of ocular prosthesis materials

Conjunctival sac microbiome in anophthalmic patients: Flora diversity and the impact of ocular prosthesis materials

Identifying oral microbiome alterations in adult betel quid chewing population of Delhi, India

The Role of Selected Habits, Periodontal Disease and Oral Hygiene Status on the Occurrence and Prognosis of Oral Mucosal Lesions

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