Alteration of optical and morphological properties of polycarbonate illuminated by visible/IR laser beams

Ehssani, H.; Saghafi, Saiedeh; Ghoranneviss, M.; Hantehzadeh, Mohammad Reza; Hosseini, Habib Mir M.; Dodt, Hans‐Ulrich

doi:10.2971/jeos.2010.10032s

Cited by 2 publications

(2 citation statements)

References 30 publications

(26 reference statements)

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“…The use of longer excitation wavelength results in reduced scatter losses and damage to tissue. 37,38 Due to the short fluorescence lifetime of Myo-mCherry, our O 2 -sensing system is compatible with the highest scan rates available. We used TCSPC collection for this data, but high scan rate FLIM should obtain the same information.…”

Section: Resultsmentioning

confidence: 99%

Intracellular oxygen mapping using a myoglobin-mCherry probe with fluorescence lifetime imaging

et al. 2018

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Oxygen (O 2) is one of the most important biometabolites. In abundance, it serves as the limiting terminus of aerobic respiratory chains in the mitochondria of higher organisms; in deficit, it is a potent determinant of development and regulation of other physiological and therapeutic processes. Most knowledge on intracellular and interstitial concentration ([O 2 ]) is derived from mitochondria isolated from cells or tissue biopsies, providing detailed but nonnative insight into respiratory chain function. The possible loss of essential metabolites during isolation and disruption of the normal interactions of the organelle with the cytoskeleton may cause these data to misrepresent intact cells. Several optical methodologies were also developed, but they are often unable to detect heterogeneity of metabolic characteristics among different individual cells in the same culture, and most cannot detect heterogeneous consumption within different areas of a single cell. Here, we propose a noninvasive and highly sensitive fluorescence lifetime microscopy probe, myoglobin-mCherry, appropriate to intracellular targeting. Using our probe, we monitor mitochondrial contributions to O 2 consumption in A549 nonsmall cell lung cancer cells and we reveal heterogeneous [O 2 ] within the intracellular environments. The mitochondrial [O 2 ] at a single-cell level is also mapped by adding a peptide to target the probe to the mitochondria.

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Section: Resultsmentioning

confidence: 99%

Intracellular oxygen mapping using a myoglobin-mCherry probe with fluorescence lifetime imaging

et al. 2018

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“…At the moment significant attention is given to those sources that bring together high power and compactness. Such high power lasers find important applications for material treatments and such applications are presented by Ehsani et al [1] and Saiedeh Saghafi et al [2], in the treatment of dielectric thin films (Alteration of optical and morphological properties of polycarbonate illuminated by visible/IR laser beams) or of biological tissues like pistachio seeds (Investigating the effects of laser beams (532 and 660 nm) in annihilation of pistachio mould fungus using spectrophotometry analysis). In particular the latter paper shows how laser sources can find very important applications in new domains, preserving goods and food without the need for preservatives or pesticides by simply sterilizing them using light.…”

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confidence: 99%