Alteration of muscarinic and purinergic receptors in urinary bladder of rats with cyclophosphamide-induced interstitial cystitis

Kageyama, Aiko; Fujino, Tomomi; Taki, Yuko; Kato, Yoshihisa; Nozawa, Yoshihisa; Ito, Yoshihiko; Yamada, Shizuo

doi:10.1016/j.neulet.2008.02.065

Cited by 24 publications

(21 citation statements)

References 23 publications

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“…The CYP treatment compared with the sham treatment caused a significant (36%) decrease in B max for specific [ 3 H]NMS binding in the rat bladder, with little effect on the K d (Table ). This was consistent with our previous findings . Moreover, there was little change in the K d or B max for specific [ 3 H]NMS binding in the submaxillary gland, suggesting a specific downregulation of bladder muscarinic receptors.…”

Section: Resultssupporting

confidence: 93%

“…The radioligand binding assays for muscarinic and P2X receptors were performed using [ 3 H]NMS and [ 3 H] αβ ‐MeATP as previously described . In the [ 3 H]NMS binding assay, the tissue homogenates was incubated with different concentrations (0.06–1.0 nM) of [ 3 H]NMS in 30 mM Na + /HEPES buffer (pH 7.5).…”

Section: Methodsmentioning

confidence: 99%

“…1,7 -11 Our group also revealed that treatment with CYP or HCl leads to a decrease in the number of muscarinic and purinergic receptors in rat bladder. 12,13 Thus, the alteration of muscarinic and purinergic receptors in the bladder appears to be a common pathway in the pathogenesis of chemical cystitis and might serve as direct therapeutic target for the development of targeted therapy for IC. Although many causes have been proposed for IC, strong evidence supports a central role for inflammation in the propagation of symptoms.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide‐Induced Cystitis

Nasrin

Masuda

Kugaya

et al. 2013

LUTS

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Section: Resultssupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide‐Induced Cystitis

Nasrin

Masuda

Kugaya

et al. 2013

LUTS

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“…The study found that A-317491 and another nonselective purinergic receptor antagonist, PPADS (pyridoxal-phosphate-6-azophenyl-2′,4′-disulfonate), were effective in ameliorating the detrusor overactivity. Another study supported these findings [28]. …”

Section: Purinergic Signaling In Interstitial Cystitis/painful Bladdesupporting

confidence: 56%

Role of Purinergic Signaling in Voiding Dysfunction

Sun

Chai

2010

Curr Bladder Dysfunct Rep

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Purinergic signaling is a term that relates to adenosine triphosphate binding to its receptor (purinergic receptors such as P2X and P2Y subtypes). This pathway has been implicated in bladder functional disorders related to interstitial cystitis/painful bladder syndrome, neurogenic bladder secondary to spinal cord injury, lower urinary tract symptoms, diabetes, and aging. Purinergic signaling occurs at multiple sites, including the central nervous system, peripheral motor and sensory nerves, detrusor smooth muscle, and bladder urothelium. Future pharmacologic agents to treat bladder functional disorders may be able to target purinergic signaling at one or more of these sites.

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“…Previous studies have implied that urinary cytokines or chemokines are involved in CYP-induced cystitis (4) and in patients with cystitis (5). We recently showed that treatment with CYP or hydrochloric acid caused a decrease in the number of muscarinic and purinergic receptors in the rat bladder (6,7). Therefore, urinary cytokines and pharmacologically relevant bladder receptors may serve as direct therapeutic targets or potential biomarkers for the development of targeted therapy designed to prevent chronic bladder inflammatory conditions such as IC.…”

Section: Introductionmentioning

confidence: 99%

Beneficial Effects of Gosha-jinki-gan and Green Tea Extract in Rats With Chemical Cystitis

Nasrin¹,

Osano²,

Ito³

et al. 2013

J Pharmacol Sci

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Abstract. The aim of this study was to characterize pharmacological effects of gosha-jinki-gan (GJG) and green tea extract (GTE), on urodynamic parameters, bladder receptors, and urinary cytokines in rats with cyclophosphamide (CYP)-induced cystitis. Urodynamic parameters in CYPtreated rats were measured using the cystometric method. Muscarinic and purinergic receptors in rat tissues were measured by radioreceptor assays. Urinary cytokine levels were measured with ELISA kits. GJG and GTE were orally administered to rats once a day for 7 days. The GJG treatment significantly ameliorated changes in urodynamic parameters in CYP-treated rats. Similar treatment with GTE slightly attenuated changes in urodynamic parameters. The maximal number of binding sites for [ H]NMS and [3 H]ab-MeATP in the bladder was significantly lower in CYPtreated rats than in sham rats. Such a reduction in receptor density was significantly attenuated by the GJG treatment. GTE treatment also significantly attenuated the down-regulation of muscarinic receptors, but not P2X receptors in bladders of rats with CYP-induced cystitis. The elevation in urinary cytokine levels in CYP-treated rats was effectively attenuated by GJG treatment. The elevation in cytokine levels in CYP-treated rats was alleviated by GTE treatment. In conclusion, GJG may be a pharmacologically useful plant extract for cystitis.

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Alteration of muscarinic and purinergic receptors in urinary bladder of rats with cyclophosphamide-induced interstitial cystitis

Cited by 24 publications

References 23 publications

Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide‐Induced Cystitis

Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide‐Induced Cystitis

Role of Purinergic Signaling in Voiding Dysfunction

Beneficial Effects of Gosha-jinki-gan and Green Tea Extract in Rats With Chemical Cystitis

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