Alteration of human macrophages microRNA expression profile upon infection with Mycobacterium tuberculosis

Furci, Lucinda; Schena, Elisa; Miotto, Paolo; Cirillo, Daniela María

doi:10.1016/j.ijmyco.2013.04.006

Cited by 58 publications

(39 citation statements)

References 33 publications

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“…Together, these studies indicate that the expression of multiple miRNAs is upregulated post-mycobacterial infection, which may benefit host defense mechanisms against mycobacteria. However, previous studies found that the expression of miR-27b in human monocyte-derived macrophages (MDMs) was different from that stated in our data (35). The contradiction in the expression of miR-27b between our findings using macrophages and those of others using human MDMs may be due to the difference in cell types, time of infection, and growth phases of the bacteria.…”

Section: Discussioncontrasting

confidence: 99%

MicroRNA-27b Modulates Inflammatory Response and Apoptosis duringMycobacterium tuberculosisInfection

Liang

Song

et al. 2018

The Journal of Immunology

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poses a significant global health threat. MicroRNAs play an important role in regulating host anti-mycobacterial defense; however, their role in apoptosis-mediated mycobacterial elimination and inflammatory response remains unclear. In this study, we explored the role of microRNA-27b (miR-27b) in murine macrophage responses to infection. We uncovered that the TLR-2/MyD88/NF-κB signaling pathway induced the expression of miR-27b and miR-27b suppressed the production of proinflammatory factors and the activity of NF-κB, thereby avoiding an excessive inflammation during infection. Luciferase reporter assay and Western blotting showed that miR-27b directly targeted Bcl-2-associated athanogene 2 (Bag2) in macrophages. Overexpression of Bag2 reversed miR-27b-mediated inhibition of the production of proinflammatory factors. In addition, miR-27b increased p53-dependent cell apoptosis and the production of reactive oxygen species and decreased the bacterial burden. We also showed that Bag2 interacts with p53 and negatively regulates its activity, thereby controlling cell apoptosis and facilitating bacterial survival. In summary, we revealed a novel role of the miR-27b/Bag2 axis in the regulation of inflammatory response and apoptosis and provide a potential molecular host defense mechanism against mycobacteria.

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Section: Discussioncontrasting

confidence: 99%

MicroRNA-27b Modulates Inflammatory Response and Apoptosis duringMycobacterium tuberculosisInfection

Liang

Song

et al. 2018

The Journal of Immunology

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“…Recent reports have shown differential expression of host microRNA (miRNA) in response to mycobacterial exposure [20][21][22][23]. Using a set of filtration methods, we retrieved information from several databases to identify a set of miRNAs that target the 43 DMGs.…”

Section: Justification Of the Bcg Responder Module By Validation Agaimentioning

confidence: 99%

Identification of DNA methylation patterns predisposing for an efficient response to BCG vaccination in healthy BCG-naïve subjects

et al. 2019

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The protection against tuberculosis induced by the Bacille Calmette Guérin (BCG) vaccine is unpredictable. In our previous study, altered DNA methylation pattern in peripheral blood mononuclear cells (PBMCs) in response to BCG was observed in a subgroup of individuals, whose macrophages killed mycobacteria effectively (‘responders’). These macrophages also showed production of Interleukin-1β (IL-1β) in response to mycobacterial stimuli before vaccination. Here, we hypothesized that the propensity to respond to the BCG vaccine is reflected in the DNA methylome. We mapped the differentially methylated genes (DMGs) in PBMCs isolated from responders/non-responders at the time point before vaccination aiming to identify possible predictors of BCG responsiveness. We identified 43 DMGs and subsequent bioinformatic analyses showed that these were enriched for actin-modulating pathways, predicting differences in phagocytosis. This could be validated by experiments showing that phagocytosis of mycobacteria, which is an event preceding mycobacteria-induced IL-1β production, was strongly correlated with the DMG pattern.

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“…Recent studies report an altered expression of miRNA-146 and miRNA-155 in case of Helicobacter pylori infection [ 76 ], Listeria monocytogenes [ 77 ], Mycobacterium tuberculosis [ 78 ], and Salmonella enterica [ 79 ].…”

Section: Circulating Mirnas As Biomarker For Sepsismentioning

confidence: 99%

Use of miRNAs as Biomarkers in Sepsis

Dumache

Rogobete

Bedreag

et al. 2015

Analytical Cellular Pathology

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Sepsis is one of the most common causes of death in critical patients. Severe generalized inflammation, infections, and severe physiological imbalances significantly decrease the survival rate with more than 50%. Moreover, monitoring, evaluation, and therapy management often become extremely difficult for the clinician in this type of patients. Current methods of diagnosing sepsis vary based especially on the determination of biochemical-humoral markers, such as cytokines, components of the complement, and proinflammatory and anti-inflammatory compounds. Recent studies highlight the use of new biomarkers for sepsis, namely, miRNAs. miRNAs belong to a class of small, noncoding RNAs with an approximate content of 19–23 nucleotides. Following biochemical and physiological imbalances, the expression of miRNAs in blood or other body fluids changes significantly. Moreover, its stability, specificity, and selectivity make miRNAs ideal candidates for sepsis biomarkers. In conclusion, we can affirm that stable species of circulating miRNAs represent potential biomarkers for monitoring the evolution of sepsis.

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Alteration of human macrophages microRNA expression profile upon infection with Mycobacterium tuberculosis

Abstract: This study signifies the miRNA host response upon intracellular mycobacterial infection in macrophages, providing new aspects of regulation in host-pathogen interactions, at post-transcriptional levels.

Cited by 58 publications

References 33 publications

MicroRNA-27b Modulates Inflammatory Response and Apoptosis duringMycobacterium tuberculosisInfection

MicroRNA-27b Modulates Inflammatory Response and Apoptosis duringMycobacterium tuberculosisInfection

Identification of DNA methylation patterns predisposing for an efficient response to BCG vaccination in healthy BCG-naïve subjects

Use of miRNAs as Biomarkers in Sepsis

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