Alteration of hepatic glutathione peroxidase and superoxide dismutase expression in streptozotocin-induced diabetic mice by berberine

Lao-ong, Thinnakorn; Chatuphonprasert, Waranya; Nemoto, Nobuo; Jarukamjorn, Kanokwan

doi:10.3109/13880209.2012.655377

Cited by 61 publications

(64 citation statements)

References 29 publications

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“…Neither berberine nor glibenclamide showed a negative effect on the blood glucose AUC level of the normal mice (Table 2). These observations supported the hypoglycemic potential of berberine on the DM type 2 (Lao-ong et al, 2012;Zhang et al, 2008Zhang et al, , 2011.…”

Section: Effect Of Berberine On the Level Of Fasting Blood Glucosesupporting

confidence: 76%

“…Hepatic total RNA was reverse-transcribed using ReverTraAce Õ reverse transcriptase and then the cDNA was amplified under the conditions recommended by the supplier of Invitrogen Õ (Life Technologies Corporation, Carlsbad, CA). The conditions of PCR cycle were followed by the method of Mouatassim et al (1999), Chatuphonprasert et al (2012) and Lao-ong et al (2012). After separation of the PCR products by 2% agarose gel electrophoresis, the target cDNA were detected under ultraviolet light in the presence of Novel Juice of GeneDirex Õ (Bio-Helix Co., Ltd., Taiwan) and semi-quantified by Syngene Õ gel documentation (Ingenius L, Cambridge, UK) and the GeneTools match program (Syngene Õ ).…”

Section: Expression Of Sod and Cat Mrnasmentioning

confidence: 99%

“…Moreover, berberine restored the levels of mRNA and enzyme activities of GPx, SOD and glutathione contents in the livers of DM type 2-induced mice. The study noted the potential of berberine as a hypoglycemic agent with the added benefit of an antioxidant effect (Lao-ong et al, 2012). However, the data of berberine on oxidative stress system in other organs and information of co-treatment with other drugs were not yet completed.…”

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confidence: 99%

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Improvement of superoxide dismutase and catalase in streptozotocin–nicotinamide-induced type 2-diabetes in mice by berberine and glibenclamide

Chatuphonprasert

Lao-ong

Jarukamjorn

2013

Pharmaceutical Biology

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Context: Diabetes mellitus (DM) type 2 is a chronic disease characterized by hyperglycemia and insulin resistance. Oxidative stress participates in development and progression of DM, in which changes of superoxide dismutase (SOD) and catalase (CAT) were noted in DM mice. Berberine has been widely used as an alternative medicine and proved to be effective for the treatment of DM and dyslipidemia. Objective: Impacts of berberine on transcriptional regulation of SOD and CAT and their enzyme activities, including the level of malondialdehyde (MDA) formation, were examined in the DM type 2-induced mice to clarify its antioxidation potential, compared with a common hypoglycemic drug, glibenclamide. Materials and methods: Noninsulin-dependent diabetes was induced in mice by a single intraperitoneal streptozotocin-nicotinamide injection. Diabetic mice were treated daily with glibenclamide (10 mg/kg/d) and/or berberine (100 mg/kg/d) for 2 weeks. The fasting blood glucose and the MDA levels in the mouse liver, brain and kidneys were monitored using Glucometer Õ (Accu-Check Õ Advantage II Performa kits, Roche Diagnostics, Germany) and thiobarbituric acid substance assay, respectively. The expression of SOD and CAT mRNA were determined in the mouse liver and the activities of SOD and CAT enzymes were determined in mouse liver, brain and kidneys, respectively. Results: Berberine exhibited similar hypoglycemic potential as glibenclamide to lower area under the curve of the fasting blood glucose. In DM type 2 mice, berberine increased the hepatic CuZn-SOD mRNA expression and the kidney SOD and CAT activities to normal levels. Moreover, DM-induced lipid peroxidation by increasing of MDA levels in both the liver and brain and lipid peroxidation status was restored by berberine. Conclusion: Berberine possessed hypoglycemic properties and strong potential to improve the oxidant-antioxidant balance, though the combination treatment of berberine and glibenclamide did not show additional benefit over the treatment with berberine alone.

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Section: Effect Of Berberine On the Level Of Fasting Blood Glucosesupporting

confidence: 76%

Section: Expression Of Sod and Cat Mrnasmentioning

confidence: 99%

mentioning

confidence: 99%

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Improvement of superoxide dismutase and catalase in streptozotocin–nicotinamide-induced type 2-diabetes in mice by berberine and glibenclamide

Chatuphonprasert

Lao-ong

Jarukamjorn

2013

Pharmaceutical Biology

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“…BBR also demonstrates to have antioxidative activities in cultured cells and animals, and this effect may help reducing reactive oxygen species (ROS) production in the liver [59, 60], thus preventing liver damage. Extrahepatic factors might also affect NAFLD pathology, and in adipocytes, studies illustrate that BBR inhibits adipogenesis in murine-derived 3T3-L1 preadipocytes and human white preadipocytes [61], while enhancing glucose and fatty acid uptake by muscle cells has been proved [62], BBR is proved to play a role in pancreatic islets as well [24].…”

Section: Mechanisms Of Bbr In Nafldmentioning

confidence: 99%

Update on Berberine in Nonalcoholic Fatty Liver Disease

Liu

Zhang

Song

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Berberine (BBR), an active ingredient from nature plants, has demonstrated multiple biological activities and pharmacological effects in a series of metabolic diseases including nonalcoholic fatty liver disease (NAFLD). The recent literature points out that BBR may be a potential drug for NAFLD in both experimental models and clinical trials. This review highlights important discoveries of BBR in this increasing disease and addresses the relevant targets of BBR on NAFLD which links to insulin pathway, adenosine monophosphate-activated protein kinase (AMPK) signaling, gut environment, hepatic lipid transportation, among others. Developing nuanced understanding of the mechanisms will help to optimize more targeted and effective clinical application of BBR for NAFLD.

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“…Over the past two decades our increased understanding of the pathogenesis of this disease has led to the development of new treatments. Oxidative stress has been implicated in the T1D pathogenesis (Bhatti et al, 2011;Lao-ong et al, 2012). In recent years, there has been a growing interest in the antioxidant defense system as a regulator of disease development.…”

Section: Introductionmentioning

confidence: 99%

The ameliorative effect ofCetraria islandicaagainst diabetes-induced genetic and oxidative damage in human blood

Çolak

Geyikoğlu

Türkez

et al. 2013

Pharmaceutical Biology

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Context: The aqueous extracts of Cetraria islandica (L.) Ach. (Parmeliaceae) is traditionally used in many countries against a number of conditions, including inflammatory conditions. Objective: The present study aimed to assess, for the first time, the effectiveness of C. islandica in cultured primary blood cells of Type 1 diabetes subjects. Materials and methods: Diabetic and control blood samples were treated with or without aqueous lichen extract (5 and 10 mg mL À1) for 48 h. The activity of antioxidant enzymes in erythrocytes and also malondialdehyde levels in plasma were determined to evaluate the oxidative status. DNA damages were analyzed by SCE, MN and comet assays in cultured human lymphocytes. Additionally, proliferation index (PI) was evaluated in peripheral blood lymphocytes. Results: There were significant increases in observed total DNA damage (comet assay) (240.2%) and SCE (168.8%), but not in MN frequencies of cultures with diabetes as compared (p40.05) to controls. Whereas, the significant reductions of total DNA damage (69.2 and 65.3%) and SCE frequencies (17.7 and 12.3%) were determined when the 5 and 10 mg mL À1 lichen extract was added to the cell culture medium, respectively. However, lichen extract did not completely inhibit the induction of SCEs in lymphocytes of patients with diabetes. C. islandica extract was also useful on PI rates. Discussion: In conclusion, the antioxidant role of C. islandica in alleviating diabetes-induced genomic instability and for increasing cell viability was firstly indicated in the present study.

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Alteration of hepatic glutathione peroxidase and superoxide dismutase expression in streptozotocin-induced diabetic mice by berberine

Abstract: Berberine conveyed antioxidative effect via down- and up-regulation of GPx and CuZn-SOD expression, respectively. Therefore, use of berberine as a hypoglycemic compound for alternative treatment of DM could bring extra-beneficent consequence according to its antioxidative stress.

Cited by 61 publications

References 29 publications

Improvement of superoxide dismutase and catalase in streptozotocin–nicotinamide-induced type 2-diabetes in mice by berberine and glibenclamide

Improvement of superoxide dismutase and catalase in streptozotocin–nicotinamide-induced type 2-diabetes in mice by berberine and glibenclamide

Update on Berberine in Nonalcoholic Fatty Liver Disease

The ameliorative effect ofCetraria islandicaagainst diabetes-induced genetic and oxidative damage in human blood

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