Alteration of gut microbiome and metabolome by Clostridium butyricum can repair the intestinal dysbiosis caused by antibiotics in mice

Liu, Xin; Qiu, Xiaoyu; Yang, Yong; Wang, Jing; Wang, Qi; Liu, Jingbo; Yang, Feiyun; Liu, Zuohua; Qi, Renli

doi:10.1016/j.isci.2023.106190

Cited by 4 publications

(2 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The strain was found to produce endospores and have a butyric acid production of 2.15 mg/mL during the exponential growth phase. Additionally, it is non-pathogenic to animals and can effectively restore antibiotic-induced intestinal dysbiosis and damage in mice by rebuilding the gut microbiota and optimizing metabolic function ( Liu et al, 2023 ). Thus, it appears that CBX 2021 shows promise for application in the field of feed probiotics.…”

Section: Introductionmentioning

confidence: 99%

Uncovering the mechanism of Clostridium butyricum CBX 2021 to improve pig health based on in vivo and in vitro studies

Liu,

Qiu,

Yang

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionAs a symbiotic probiotic for the host, Clostridium butyricum (CB) has the potential to strengthen the body’s immune system and improve intestinal health. However, the probiotic mechanism of CB is not completely understood. The Clostridium butyricum CBX 2021 strain isolated by our team from a health pig independently exhibits strong butyric acid production ability and stress resistance. Therefore, this study comprehensively investigated the efficacy of CBX 2021 in pigs and its mechanism of improving pig health.MethodsIn this study, we systematically revealed the probiotic effect and potential mechanism of the strain by using various methods such as microbiome, metabolites and transcriptome through animal experiments in vivo and cell experiments in vitro.ResultsOur in vivo study showed that CBX 2021 improved growth indicators such as daily weight gain in weaned piglets and also reduced diarrhea rates. Meanwhile, CBX 2021 significantly increased immunoglobulin levels in piglets, reduced contents of inflammatory factors and improved the intestinal barrier. Subsequently, 16S rRNA sequencing showed that CBX 2021 treatment implanted more butyric acid-producing bacteria (such as Faecalibacterium) in piglets and reduced the number of potentially pathogenic bacteria (like Rikenellaceae RC9_gut_group). With significant changes in the microbial community, CBX 2021 improved tryptophan metabolism and several alkaloids synthesis in piglets. Further in vitro experiments showed that CBX 2021 adhesion directly promoted the proliferation of a porcine intestinal epithelial cell line (IPEC-J2). Moreover, transcriptome analysis revealed that bacterial adhesion increased the expression of intracellular G protein-coupled receptors, inhibited the Notch signaling pathway, and led to a decrease in intracellular pro-inflammatory molecules.DiscussionThese results suggest that CBX 2021 may accelerate piglet growth by optimizing the intestinal microbiota, improving metabolic function and enhancing intestinal health.

show abstract

Section: Introductionmentioning

confidence: 99%

Uncovering the mechanism of Clostridium butyricum CBX 2021 to improve pig health based on in vivo and in vitro studies

Liu,

Qiu,

Yang

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Probiotics, as microorganisms that help restore the balance of gut microbiota, are vital for maintaining human health (Liu et al, 2022). Commonly utilized probiotics comprise yeast, lactobacilli , and bifidobacteria , whereas Clostridium butyricum is a spore‐forming bacterium and an emerging member with great potential (Li et al, 2016; Liang et al, 2021; Liu et al, 2023). With the complete prohibition of antibiotics as feed additives, C. butyricum has garnered substantial attention for its promising role as an antibiotic alternative due to its notable benefits for animal health (Li, Wang, et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Enhancing and monitoring spore production in Clostridium butyricum using pH‐based regulation strategy and a robust soft sensor based on back‐propagation neural networks

Xu,

Zhang,

Wang

et al. 2023

Biotech & Bioengineering

View full text Add to dashboard Cite

Clostridium butyricum (C. butyricum) is a probiotic that forms anaerobic spores and plays a crucial role in regulating gut microbiota. However, the total viable cell count and spore yield of C. butyricum in industrial production are comparatively low. To this end, we investigated the metabolic characteristics of the strain and proposed three distinct pH regulation strategies for enhancing spore production. In addition, precise measurement of fermentation parameters such as substrate concentration, total viable cell count, and spore concentration is crucial for successful industrial probiotics production. Nevertheless, online measurement of these intricate parameters in the fermentation of C. butyricum poses a considerable challenge owing to the complex, nonlinear, multivariate, and strongly coupled characteristics of the production process. Therefore, we analyzed the capacitance and conductivity acquired from a viable cell sensor as the core parameters for the fermentation process. Subsequently, a robust soft sensor was developed using a seven‐input back‐propagation neural network model with input variables of fermentation time, capacitance, conductivity, pH, initial total sugar concentration, ammonium ion concentration, and calcium ion concentration. The model enables the online monitoring of total viable biomass count, substrate concentrations, and spore yield, and can be extended to similar fermentation processes with pH changes as a characteristic feature.This article is protected by copyright. All rights reserved.

show abstract

Dynamic changes of gut microbiota composition during the intervention of apple polyphenols extract to alleviate high-carbohydrate-diet induced body weight gain

Wu,

Wang,

Xie

et al. 2024

Food Bioscience

View full text Add to dashboard Cite

Alteration of gut microbiome and metabolome by Clostridium butyricum can repair the intestinal dysbiosis caused by antibiotics in mice

Cited by 4 publications

References 58 publications

Uncovering the mechanism of Clostridium butyricum CBX 2021 to improve pig health based on in vivo and in vitro studies

Uncovering the mechanism of Clostridium butyricum CBX 2021 to improve pig health based on in vivo and in vitro studies

Enhancing and monitoring spore production in Clostridium butyricum using pH‐based regulation strategy and a robust soft sensor based on back‐propagation neural networks

Dynamic changes of gut microbiota composition during the intervention of apple polyphenols extract to alleviate high-carbohydrate-diet induced body weight gain

Contact Info

Product

Resources

About