Alteration of Gastrin‐producing Cells in Rat Antral Mucosa After Truncal Vagotomy

Murakami, Shinichi; Nakayama, Iwao; Uchida, Yuzo

doi:10.1111/j.1440-1827.1988.tb02356.x

Cited by 5 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…19 Primary antibodies used were anti-alpha-1-antitrypsin (AAT) (prediluted, Dako, Carpinteria, California, U.S.A.) polyclonal and anti-neuron specific enolase (NSE) (prediluted, Biomeda, Foster City, California, U.S.A.) monoclonal antibody. Furthermore, antichromogranin A (1:100), antiinsulin (1:300), antisomatostatin (1:300), antiglucagon (1:600) and antipancreatic polypeptides (1:600) polyclonal antibodies (Dako), antigastrin polyclonal antibody produced in our laboratory, 14 and antiserotonin monoclonal antibody (Dako, 1:10) were used for the tissue sections of all cases of SCT to eliminate the possibility of islet cell tumor. As a negative control, nonimmunized rabbit or mouse immunoglobulins were used instead of the primary antibodies, and negative results were obtained.…”

Section: Methodsmentioning

confidence: 99%

Cytologic Features of Solid and Cystic Tumor of the Pancreas

Kashima

Hayashida²,

Yokoyama³

et al. 1997

Acta Cytologica

Self Cite

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Cytologic Features of Solid and Cystic Tumor of the Pancreas

Kashima

Hayashida²,

Yokoyama³

et al. 1997

Acta Cytologica

Self Cite

View full text Add to dashboard Cite

“…Several reports have shown an elevation in serum gastrin levels and hyperplasia of gastrin-producing cells (G-cells) following vagotomy in both experimental models and humans. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] In association with high serum gastrin levels, hyperplasia of certain cell organelles within G-cells has also been described in a few Reprint requests to: H. Shimoda (Received for publication on Feb. 7,1991; accepted on May 1, 1992) reports. 2-4 The mechanism of G-cell hyperplasia in the rat antral mucosa after truncal vagotomy was reported in our previous study using double immunostaining for bromodeoxyuridine (BrdU) and little gastrin (G17).…”

Section: Introductionmentioning

confidence: 99%

The proliferative kinetics of somatostatin-producing cells in the rat antral mucosa after truncal vagotomy

et al. 1992

Self Cite

View full text Add to dashboard Cite

The kinetics of somatostatin-producing cells (D-cells) in the rat antral mucosa after truncal vagotomy were studied using double immunostaining for bromodeoxyuridine (BrdU) and somatostatin. Both the concentration of somatostatin and D-cell density in the antral mucosa demonstrated a significant increase on the 3rd day after truncal vagotomy. With the single labeling of BrdU, a few D-cells showed positive immunostaining for BrdU throughout the experimental period in both vagotomized and sham operated rats. With cumulative labeling, the BrdU labeled cells demonstrated a linear increase in an identical number for each experimental time-point in both groups. The labeling index of BrdU in the D-cells increased significantly, beginning on the 3rd day and attaining a maximum level of 41.8% on the 10th day, in the vagotomized group after cumulative labeling. In this group, however, the density of D-cells with no immunoreactive BrdU also increased quickly on the 3rd day with cumulative labeling. The present study indicates that the most important factor involving D-cell hyperplasia observed after truncal vagotomy is the activation of pre-existing D-cells to synthesize and release hormones, together with the rapid replication of progenitor cells and their maturation to D-cells.

show abstract

Alteration in gastric nerve fibers containing gastrin-releasing peptide in relation to the gastrin-producing cell population after truncal vagotomy in a rat model

et al. 1995

View full text Add to dashboard Cite

The fine structural alteration in the gastric nerve fibers containing gastrin-releasing peptide (GRP) was studied in relation to the dynamics of gastrin-producing cells (G-cells) after truncal vagotomy in a rat model. The circulating gastrin levels were markedly elevated from the 1st day after vagotomy and the number of G-cells with positive immunoreaction for G17 and G34(1-15) was significantly increased in the vagotomized group. On the 3rd day after vagotomy, the G-cells showed conspicuous ultrastructural changes characterized by hypertrophy of the Golgi complexes and increased numbers of secretory granules. The GRP-positive nerve fibers formed a fine network in the gastric wall and were densely distributed in the oxyntic mucosa close to the blood vessels and showing varicosities composed of either small clear or GRP-positive large vesicles containing an electron-dense core. In the oxyntic mucosa of the vagotomized rats, axonal swelling of the nerves occurred on the 3rd day, and a depletion of GRP immunoreactivity was evidenced by a markedly decreased number of large-cored vesicles on the 7th day, when the serum GRP levels were also found to be markedly elevated. These findings indicate that the alteration in gastric nerve fibers containing GRP after truncal vagotomy may be related to hypergastrinemia and antral G-cell hyperplasia in the rat gastric mucosa.

show abstract

Alteration of Gastrin‐producing Cells in Rat Antral Mucosa After Truncal Vagotomy

Cited by 5 publications

References 23 publications

Cytologic Features of Solid and Cystic Tumor of the Pancreas

Cytologic Features of Solid and Cystic Tumor of the Pancreas

The proliferative kinetics of somatostatin-producing cells in the rat antral mucosa after truncal vagotomy

Alteration in gastric nerve fibers containing gastrin-releasing peptide in relation to the gastrin-producing cell population after truncal vagotomy in a rat model

Contact Info

Product

Resources

About