Alteration of CYP2E1, DBN1, DNMT1, miRNA-335, miRNA-21, c-Fos and Cox-2 gene expression in prefrontal cortex of rats’ offspring submitted to prenatal ethanol exposure during their neurodevelopment and the preventive role of nancocurcumin administration: A histological, ultrastructural and molecular study

Labib, Heba

doi:10.1016/j.jchemneu.2021.101940

Cited by 4 publications

(1 citation statement)

References 59 publications

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“…It has also been consistently reported to regulate several genes relevant to neural functions (e.g., BDNF , CACNA1C , and TCF4 ) ( Wright et al, 2013 ; Thomas et al, 2017 ). Prenatal stress, an environmental factor, is a risk factor for developing psychiatric disorders, including SCZ ( Cattane et al, 2020 ; Krontira et al, 2020 ), and stress also regulates expression of several miRNAs in offspring ( Liu et al, 2020a ; Mazzelli et al, 2020 ; Labib, 2021 ). However, limited studies of miRNAs have been published.…”

Section: Introductionmentioning

confidence: 99%

Impact of Gestational Haloperidol Exposure on miR-137-3p and Nr3c1 mRNA Expression in Hippocampus of Offspring Mice

Yoshino

Kumon

Shimokawa

et al. 2022

International Journal of Neuropsychopharmacology

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Background Schizophrenia is a mental disorder caused by both environmental and genetic factors. Prenatal exposure to antipsychotics, an environmental factor for the fetal brain, induces apoptotic neurodegeneration and cognitive impairment of offspring similar to schizophrenia. The aim was to investigate molecular biological changes in the fetal hippocampus exposed to haloperidol (HAL) by RNA expression as a model of the disorder. Methods HAL (1 mg/kg/day) was administered to pregnant mice. Upregulated and downregulated gene expressions in the hippocampus of offspring were studied with RNA-seq and validated with the qPCR method, and miRNA regulating mRNA expressional changes was predicted by in silico analysis. An in vitro experiment was used to identify the miRNA using a dual-luciferase assay. Results There were significant gene expressional changes (1,370 upregulated and 1,260 down regulated genes) in the HAL group compared to the control group on RNA-seq analysis (p < 0.05 and q < 0.05). Of them, the increase of Nr3c1 mRNA expression was successfully validated, and in silico analysis predicted that microRNA-137-3p (miR-137-3p) possibly regulates that gene’s expression. The expression of miR-137-3p in the hippocampus of offspring was significantly decreased in the first generation, but it increased in the second generation. In vitro experiments with Neuro2a cells showed that miR-137-3p inversely regulated Nr3c1 mRNA expression, which was upregulated in the HAL group. Conclusions These findings will be keys for understanding the impact of the molecular biological effects of antipsychotics on the fetal brain.

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