Alteration of chemokine production in bovine endometrial epithelial and stromal cells under heat stress conditions

Sakai, Shunsuke; Hatabu, Toshimitsu; Yamamoto, Yuki; Kimura, Koji

doi:10.14814/phy2.14640

Cited by 9 publications

(10 citation statements)

References 49 publications

(66 reference statements)

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“…To recover from endometritis, endometrial epithelial and stromal cells secrete the IL‐6 to recruit macrophages into infection site, to induce the inflammation, and to activate the neutrophils (Ataie‐Kachoie et al, 2014; Clahsen & Schaper, 2008; Hurst et al, 2001; Jones et al, 2006; Saut et al, 2014; Sheldon et al, 2014). Recent our study showed that hyperthermia induces the IL6 mRNA expression and IL‐6 secretion responsive to LPS in the bovine endometrial stromal cells while those in the bovine endometrial epithelial cells are suppressed, and consequently affects the localization of macrophages in the endometrium (Sakai et al, 2020). Here, we focused on the mechanisms for the alteration of IL‐6 production in the two types of endometrial cells in the presence of LPS under hyperthermia conditions.…”

Section: Discussionmentioning

confidence: 95%

“…Recent our study showed that hyperthermia induces the IL6 mRNA expression and IL-6 secretion responsive to LPS in the bovine endometrial stromal cells while those in the bovine endometrial epithelial cells are suppressed, and consequently affects the localization of macrophages in the endometrium (Sakai et al, 2020). Here, we focused on the mechanisms for the alteration of IL-6 production in the two types of endometrial cells in the presence of LPS under hyperthermia conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, after both types of endometrial cells were exposed to the control (38.5°C) or hyperthermia (40.5°C) conditions, these cells were challenged by LPS for 6 h. After the culture, these cells were used for analysis by RNA‐seq. We previously revealed that hyperthermia and LPS challenge do not affect the cell viability (Sakai et al, 2020). In addition, we also confirmed there were no effects of hyperthermia and LPS challenge on the cell viability in the present study (data not shown).…”

Section: Methodsmentioning

confidence: 98%

“…Three samples each of endometrial epithelial and stromal cells were cultured in four-well dishes. When these cells were 70% confluent, these two types of endometrial cells were cultured under control and hyperthermia conditions in the presence of LPS (1 µg/ml) (LPS from E. coli O55:B5; Sigma-Aldrich, L6529) as described previously (Sakai et al, 2020). Briefly, after both types of endometrial cells were exposed to the control (38.5°C) or hyperthermia (40.5°C) conditions, these cells were challenged by LPS for 6 h. After the culture, these cells were used for analysis by RNA-seq.…”

Section: Experiments 1: Comprehensive Analysis Of Gene Expression Pat...mentioning

confidence: 99%

“…Especially, since IL‐6 has multiple roles in the innate immune responses, such as the activation of neutrophils, induction of antimicrobial factors, stimulation of acute‐phase response, and recruitment of macrophage into infection site (Ataie‐Kachoie et al, 2014; Hurst et al, 2001; Jones et al, 2006), the regulation of IL‐6 secretion in endometrial cells is essential for recover from endometritis. However, our previous report suggested that the downregulation and upregulation of IL‐6 production in the endometrial epithelial and stromal cells in the presence of lipopolysaccharide (LPS), respectively, under hyperthermia conditions affect the localization of macrophages in endometrium (Sakai et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Hyperthermia alters interleukin‐6 production in response to lipopolysaccharide via endoplasmic reticulum stress in bovine endometrial cells

Sakai

Inoue

Tanaka

et al. 2021

Journal Cellular Physiology

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In the postpartum period, cows experience the uterine bacterial infection and develop the endometritis. To eliminate bacteria and recover from endometritis, endometrial epithelial and stromal cells secrete the cytokine and chemokine, such as interleukin 6 (IL-6), IL-8, and monocyte chemotactic protein 1 (MCP1), to recruit immune cells. Moreover, the symptom of endometritis is prolonged in summer and we have recently indicated that hyperthermia suppresses and enhances the IL-6 production in response to lipopolysaccharide (LPS) challenge in endometrial epithelial and stromal cells, respectively. However, the mechanisms for the opposite reaction of IL-6 secretion in response to LPS challenge in both types of endometrial cells under hyperthermia conditions were still unclear. To reveal these mechanisms, both types of endometrial cells were cultured with LPS under the control (38.5°C) or hyperthermia (40.5°C) conditions and comprehensively analyzed differential gene expressions of them by RNA-seq. In addition, based on these results, we examined the effect of endoplasmic reticulum (ER) stress on the IL-6 production in both types of endometrial cells cultured with LPS under hyperthermia conditions. In comprehensive analysis, hyperthermia induced the ER stress in the endometrial stromal cells but not in the endometrial epithelial cells. Actually, we confirmed that hyperthermia increased the gene expression of BiP, ATF4, and sXBP1 and protein expression of BiP and phosphorylated inositol requiring 1, ER stress marker, in the endometrial stromal cells but not in the endometrial epithelial cells. Moreover, in the endometrial stromal cells exposed to LPS, activation and inhibition of ER stress enhanced the IL-6 production under control conditions and suppressed it under hyperthermia conditions, respectively. In this study, we could uncover the one of causes for the disruption of IL-6 production in response to LPS challenge in the endometrial cells under hyperthermia conditions. This finding might be a clue for the improvement of the symptom of endometritis in cows during summer.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 98%

Section: Experiments 1: Comprehensive Analysis Of Gene Expression Pat...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hyperthermia alters interleukin‐6 production in response to lipopolysaccharide via endoplasmic reticulum stress in bovine endometrial cells

Sakai

Inoue

Tanaka

et al. 2021

Journal Cellular Physiology

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Identification and bioinformatics analysis of differentially expressed milk exosomal microRNAs in milk exosomes of heat-stressed Holstein cows

Wang

Fang

Zeng

et al. 2021

Funct Integr Genomics

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BackgroundIn summer, heat stress is one of the primary reasons for the compromised health and low milk productivity of dairy cows. Hyperthermia affects milk synthesis and secretion in the mammary glands of dairy cows. As molecules for intercellular communication, milk-derived exosomes carry genetic material, proteins and, lipids, playing a crucial role in mammary tissue growth and milk synthesis in dairy cows.The aim of this study was to explore the milk exosomal miRNAs pro le of heat-stressed and normal Holstein cows. ResultsWe isolated and identi ed milk exosomes to screening for differentially expressed miRNAs using small RNA sequencing. Then, TargetScan and miRanda algorithms were used to predict the putative targets of the differentially expressed miRNAs, whereas GO and KEGG pathway enrichment analyses were performed for the differentially expressed miRNA-target genes. Our results showed that 215 miRNAs were signi cantly differentially expressed in heat-stressed milk exosomes, of which one was up-regulated and 214 were signi cantly downregulated. GO and KEGG enrichment analyses indicated that differentially expressed miRNAs might play a role in apoptosis, autophagy, and the p38 MAPK pathway. qRT-PCR assay veri ed that the expression of miRNAs was consistent with the sequencing results, warranting further veri cation of their speci c targets of action. ConclusionsIn conclusion, changes in the miRNA expression pro le of milk exosomes indicated the role of exosomal miRNAs in regulating heat stress resistance and apoptosis in dairy cows. Our results suggested that milkderived exosomal miRNAs could increase mammary gland resistance to heat stress, thereby enhancing milk synthesis in dairy cows.

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Overexpression of USP22 ameliorates LPS-induced endometrial stromal cells inflammation and modulates cells decidualization by inhibiting ferroptosis

Xing,

Zhang,

et al. 2024

Reproductive Biology

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Alteration of chemokine production in bovine endometrial epithelial and stromal cells under heat stress conditions

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 9 publications

References 49 publications

Hyperthermia alters interleukin‐6 production in response to lipopolysaccharide via endoplasmic reticulum stress in bovine endometrial cells

Hyperthermia alters interleukin‐6 production in response to lipopolysaccharide via endoplasmic reticulum stress in bovine endometrial cells

Identification and bioinformatics analysis of differentially expressed milk exosomal microRNAs in milk exosomes of heat-stressed Holstein cows

Overexpression of USP22 ameliorates LPS-induced endometrial stromal cells inflammation and modulates cells decidualization by inhibiting ferroptosis

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