Alteration in timing of cell differentiation resulting from cell interactions during development of the cellular slime mold, Dictyostelium discoideum

Atryzek, Vladimir

doi:10.1016/0012-1606(76)90167-6

Cited by 11 publications

(2 citation statements)

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“…These issues have been examined in detail for other axenic strains and our data are consistent with these reports Zada-Hames and Ashworth, 1978a;Zada-Hames and Ashworth, 1978b). The cell division that we observe for AX4 strains is similar to that reported for AX2 and AX3 (Atryzek, 1976;Zada-Hames and Ashworth, 1978a).…”

Section: Discussionsupporting

confidence: 88%

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Tissue-specific G1-phase cell-cycle arrest prior to terminal differentiation inDictyostelium

2004

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The cell cycle status of developing Dictyostelium cells remains unresolved because previous studies have led to conflicting interpretations. We propose a new model of cell cycle events during development. We observe mitosis of about 50% of the cells between 12 and 18 hours of development. Cellular DNA content profiles obtained by flow cytometry and quantification of extra-chromosomal and chromosomal DNA suggest that the daughter cells have half the chromosomal DNA of vegetative cells. Furthermore, little chromosomal DNA synthesis occurs during development, indicating that no S phase occurs. The DNA content in cells sorted by fluorescent tissue-specific reporters indicates that prespore cells divide before prestalk cells and later encapsulate as G1-arrested spores. Consistent with this, germinating spores have one copy of their chromosomes, as judged by fluorescence in situ hybridization and they replicate their chromosomes before mitosis of the emergent amoebae. The DNA content of mature stalk cells suggests that they also attain a G1 state prior to terminal differentiation. As prestalk cells appear to be in G2 up to 22 hours of development, our data suggest that they divide just prior to stalk formation. Our results suggest tissue-specific regulation of G1 phase cell cycle arrest prior to terminal differentiation in Dictyostelium.

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Section: Discussionsupporting

confidence: 88%

“…cell divisions occur between 12 and 20 hours, after multicellular structures are formed (Atryzek, 1976;Zada-Hames and Ashworth, 1978a). The currently accepted model posits that all cells that divide during development replicate their chromosomes after mitosis and undergo terminal differentiation as G2 cells (Weeks and Weijer, 1994).…”

Section: Introductionmentioning

confidence: 99%

Tissue-specific G1-phase cell-cycle arrest prior to terminal differentiation inDictyostelium

2004

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Developmental age-related cell sorting inDictyostelium discoideum

McDonald

1984

Wilhelm Roux' Archiv

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The sorting behavior of mixtures ofD. discoideum cells which had been developed for different lengths of time was examined. Cells developed for 4 and 8 h were mixed together and allowed to form slugs. Within the slugs, 8 h cells sorted to the anterior prestalk region while 4 h cells sorted to the posterior prespore region. These results indicate that the more developed a cell is, the more likely it is to become part of the prestalk zone in the slug. They are also consistent with the differential adhesion and chemotaxis hypotheses as a mechanism for cell sorting since cells become more adhesive and chemotactically responsive as development proceeds.

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The cell cycle and its relationship to development in Dictyostelium discoideum

Hames

Ashworth

1978

Developmental Biology

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Alteration in timing of cell differentiation resulting from cell interactions during development of the cellular slime mold, Dictyostelium discoideum

Cited by 11 publications

References 11 publications

Tissue-specific G1-phase cell-cycle arrest prior to terminal differentiation inDictyostelium

Tissue-specific G1-phase cell-cycle arrest prior to terminal differentiation inDictyostelium

Developmental age-related cell sorting inDictyostelium discoideum

The cell cycle and its relationship to development in Dictyostelium discoideum

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