Alteration in rat apolipoprotein C-III gene expression and lipoprotein composition during inflammation

Shen, Peiyan; Howlett, Geoffrey J.

doi:10.1007/bf00916102

Cited by 9 publications

(8 citation statements)

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“…ApoA-IV is synthesized primarily in the intestine then secreted into the plasma, and an acute inflammation can disturb the plasma ApoA-IV concentration [Tu et al 1987;Shen and Howlett 1993]. Up-regulation of ApoA-IV in the CSF of GBS patients is in agreement with previous reports that large concentrations of ApoA-IV aggregated in the regenerating nerve for myelin biosynthesis (Langner et al 1991).…”

Section: Discussionsupporting

confidence: 81%

Comparative Proteomics Analysis of Cerebrospinal Fluid of Patients with Guillain–Barré Syndrome

et al. 2008

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To better understand the pathophysiologic mechanisms underlying Guillain-Barré syndrome (GBS), Comparative proteomic analysis of cerebrospinal fluid (CSF) between patients with GBS (the experiment group) and control subjects suffering from other neurological disorders (the control group) was carried out using two-dimensional gel electrophoresis (2-DE) technique, in combination with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and database searching to determine abnormal CSF proteins in GBS patients. Image analysis of 2-DE gels silver stained revealed that 10 protein spots showed significant differential expression between the two groups of CSF samples. The expression of cystatin C, transthyretin, apolipoprotein E and heat shock protein 70 were decreased. However, haptoglobin, alpha-1-antitrypsin, apolipoprotein A-IV and neurofilaments were elevated. The subsequent ELISA measured the concentration of cystatin C and confirmed the result of the proteomic analysis. These identified proteins may be involved in the pathophysiological process of GBS and call for further studying the role of these proteins in the pathogenesis of the disease.

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Section: Discussionsupporting

confidence: 81%

Comparative Proteomics Analysis of Cerebrospinal Fluid of Patients with Guillain–Barré Syndrome

et al. 2008

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“…Apolipoprotein A‐IV was also found significantly increased in GBS patients. ApoA‐IV is synthesized primarily in the intestine then secreted into the plasma, and an acute inflammation can disturb the plasma apoA‐IV concentration [13, 14]. The relationship between the level of apoA‐IV and inflammation has never been described in humans to the best of our knowledge.…”

Section: Discussionmentioning

confidence: 99%

Proteomic identification of potential protein markers in cerebrospinal fluid of GBS patients

Jin

Chang

et al. 2007

Euro J of Neurology

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Increased protein level in the cerebrospinal fluid (CSF) is a characteristic of patients with Guillain-Barré syndrome (GBS), an acute inflammatory autoimmune disorder in the peripheral nervous system (PNS). However, the molecular mechanisms underlying the disease remain poorly understood and so far no reliable disease-related markers are available. By comparing the CSF proteome of GBS patients with control subjects suffering from other neurological disorders, it may be possible to identify proteins that involve in the disease process and thus to study the pathogenesis of GBS. We used two-dimensional difference gel electrophoresis (2D DIGE) technique, in combination with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), to determine the abnormal CSF proteins in GBS patients. Our data showed that the levels of six proteins and their isoforms in CSF were significantly altered in GBS patients compared with controls. Haptoglobin, apolipoprotein A-IV and PRO2044 (unnamed protein) were considerably increased in the CSF of GBS patients, whereas transthyretin, apolipoprotein E and fibrinogen were considerably decreased. We concluded that these six proteins may be involved in the pathogenesis of GBS and call for further studying the role of these proteins in the pathogenesis of the disease.

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“…The present results, coupled with the previous findings, suggest that BHV-1 is less potent than Pasteurella haemolytica in decreasing the apoC-III concentration under the conditions used. The hepatic synthesis of apoC-III is regulated by cytokines [4,13]. Cytokines produced in response to infection may suppress the synthesis of apoC-III by the liver.…”

Section: Discussionmentioning

confidence: 99%

“…It is suggested to be involved in the regulation of the triglyceride metabolism [5] in the CM and VLDL and the activation of lecithin:cholesterol acyltransferase (LCAT), the enzyme responsible for esterification of cholesterol in HDL [6]. The major site for apoC-III synthesis is the liver and the synthesis is regulated by cytokines [4,13]. In contrast to apoC-III in humans and laboratory animals, its counterpart in bovines shows a unique property with respect to the distribution in lipoprotein fractions; cow apoC-III is detected mainly in HDL, not in the CM and VLDL fractions [18].…”

Section: Induction Of Pneumoniamentioning

confidence: 99%

Decreased Apolipoprotein C-III Concentration in the High-Density Lipoprotein Fraction from Calves Inoculated with Pasteurella haemolytica and Bovine Herpes Virus-1

Yamamoto

Katoh

2000

The Journal of Veterinary Medical Science

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ABSTRACT. Lipoprotein lipid and apoprotein concentrations are known to be altered during the acute-phase response. We have previously shown that the serum activity of lecithin:cholesterol acyltransferase (LCAT) and concentration of cholesteryl esters, both constituents of the high-density lipoprotein (HDL) fraction, are reduced in calves inoculated with Pasteurella haemolytica and bovine herpes virus-1, the two major pathogens for calf pneumonia. The concentration of apolipoprotein C-III (apoC-III), a low molecular mass protein component distributed mainly in the HDL fraction, was therefore examined in bacteria-and virus-inoculated calves. An enzyme-linked immunosorbent assay demonstrated that it was decreased by inoculations of Pasteurella haemolytica and bovine herpes virus-1. The decrease was detected as early as 1 day after inoculation in both groups. A decreased serum apoC-III concentration was also observed by immunoblot analysis. It was detected in the HDL fractions from the bacteria-and virus-inoculated calves, and HDL apoC-III concentrations in the inoculated calves were decreased compared with controls. These results, coupled with the previous findings on LCAT activity and the cholesteryl ester concentration, indicate that a decreased HDL concentration is one of the early events occurring during the acute-phase response evoked by infections with Pasteurella haemolytica and bovine herpes virus-1. inoculated with Pasteurella haemolytica and bovine herpes virus-1 (BHV-1), the two major pathogens for calf pneumonia [3,12,14,16,20], we have shown that LCAT activity and the cholesteryl ester concentration are decreased [10]. Because apoC-III also is distributed in the HDL fraction, it is conceivable that the apoC-III concentration is decreased in experimental pneumonia. The purpose of the study reported here was to evaluate the apoC-III concentration in calves inoculated with Pasteurella haemolytica and BHV-1, in order to elucidate the involvement of apoC-III in the pathogenesis of calf pneumonia, one of the most economically important disorders in calves. MATERIALS AND METHODS Induction of pneumonia:Pneumonia was experimentally induced by inoculations of Pasteurella haemolytica and BHV-1 into calf lung lobes, as described previously [7,8,10,17]. Briefly, a suspension of Pasteurella haemolytica (serotype 1, I29 strain; 1 × 10 9 colony forming units) was administered to right lung lobes of 10 Holstein male calves (2 to 3 months old) using a fiberoptic bronchoscope. Ten other calves receiving the vehicle (phosphate-buffered saline [PBS]) alone were used as controls. Under xylazine-and procaine-induced anesthesia, calves were exsanguinated at day 1 (3 control and 3 inoculated calves), day 2 (3 control and 3 inoculated), day 4 (2 control and 2 inoculated) and day 7 (2 control and 2 inoculated). Blood was collected at 0 (1 hr before administration) and 0.25 (6 hr after) and at 1, Apolipoprotein C-III (apoC-III) is the low molecular mass protein component of lipoproteins and is distributed in human plasma in chylo...

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Alteration in rat apolipoprotein C-III gene expression and lipoprotein composition during inflammation

Cited by 9 publications

References 31 publications

Comparative Proteomics Analysis of Cerebrospinal Fluid of Patients with Guillain–Barré Syndrome

Comparative Proteomics Analysis of Cerebrospinal Fluid of Patients with Guillain–Barré Syndrome

Proteomic identification of potential protein markers in cerebrospinal fluid of GBS patients

Decreased Apolipoprotein C-III Concentration in the High-Density Lipoprotein Fraction from Calves Inoculated with Pasteurella haemolytica and Bovine Herpes Virus-1

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