Alteration in liver lipid and lipid fatty acid composition after partial hepatectomy in the rat

Glende, Eric A.; Morgan, Winfield S.

doi:10.1016/0014-4800(68)90015-4

Cited by 52 publications

(47 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(3) pmol/g of fresh liver after partial hepatectomy, and 5.5 ± 0.6 (3) in sham-operated rat liver. This increase (in the regenerating liver compared with controls; P < 0.02) was in general agreement with the results of Glende & Morgan (1968), and is explicable by the influx to liver of fatty acids from plasma, as well as by the increased rate of synthesis de novo. In view of the established parallelism between processes of lipogenesis and desaturation (Gellhorn & Benjamin, 1966), the synthesis of unsaturated acids was followed with 3H20 in the regenerating liver (Table 3).…”

Section: Synthesis De Novo Of Liver Fatty Acids After Partial Hepatecsupporting

confidence: 89%

“…In the present experiments, this feature persisted for 2 days. The increased rates of fatty acid synthesis could have contributed to the extra fat deposited (which consists entirely of triacylglycerol; Glende & Morgan, 1968; the present work). The transient fatty nature of the liver is probably caused by the relative stress and fooddeprivation that occur for 1-2 days after partial hepatectomy (compared with either sham-operated controls or untreated animals), resulting in mobil--ization of fatty acids from adipose tissue to liver, rather than by any more subtle feature of the posthepatectomy state.…”

Section: Concentration Ofliver Glycogen Andplasma Lipidsmentioning

confidence: 69%

“…Although it is well established that protein synthesis is increased during the period of re-growth, the details of the reconstitution of lipid components (membranes, fat-droplets, lipoproteins) are not fully documented. There is usually an increased content of hepatic fat during regeneration; the extra lipid consists of triacylglycerols (Glende & Morgan, 1968), whereas phospholipid deposition (with the exception of cardiolipin) exactly keeps pace with the overall growth rate (Glende & Morgan, 1968;Tata, 1970;Fex,, 1970b;Bergelson et al, 1974;Fex & Thorzell, 1975). Triacylglycerol synthesis, at least from preformed fatty acids, is increased after resection of part of the liver, as may be demonstrated with labelled fatty acids (Fex & Olivecrona, 1968a;Olivecrona & Fex, 1970;Fex, 1970a) or glycerol (Olivecrona & Fex, 1970).…”

mentioning

confidence: 95%

See 2 more Smart Citations

Fatty acid synthesis in the regenerating liver of the rat

Gove¹,

Hems²

1978

Biochemical Journal

View full text Add to dashboard Cite

1. Synthesis de novo of fatty acids in the rat liver, measured per g wet wt. of tissue, was increased by a factor of about two, between 1 and 4 days after partial hepatectomy, compared with rates in sham-operated control rat livers. 2. There were no associated changes in the rates ofliver cholesterol synthesis or of adipose-tissue fatty acid synthesis in rats after partial hepatectomy, compared with rates in sham-operated rats. 3. In regenerating livers, perfused under three different conditions, there was no alteration in the capacity for fatty acid synthesis compared with that of control rats. 4. The increased synthesis of fatty acids in regenerating liver was associated with insignificant increases in plasma concentrations oftriacylglycerols and free fatty acids, with a decrease in content of liver glycogen, and with no change in hepatic activity of acetyl-CoA carboxylase. 5. The accelerated rate ofsynthesis offatty acids in regenerating liver appears not to be due to any intrinsic alteration in hepatic capacity for fatty acid synthesis, but it may be caused by the continuous action on liver of unidentified circulating factors.

show abstract

Section: Synthesis De Novo Of Liver Fatty Acids After Partial Hepatecsupporting

confidence: 89%

Section: Concentration Ofliver Glycogen Andplasma Lipidsmentioning

confidence: 69%

mentioning

confidence: 95%

See 1 more Smart Citation

Fatty acid synthesis in the regenerating liver of the rat

Gove¹,

Hems²

1978

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…Hepatocytes accumulate triacylglycerol and cholesteryl esters in these lipid droplets during liver regeneration. 15,36 The levels of hepatic cholesterol and triglycerides increase 10-fold 36 during the first 24 hours after partial hepatectomy, which coincides with an increased number of peroxisomes. 37 The lipid droplets are usually detected in the vicinity of peroxisomes.…”

Section: Discussionmentioning

confidence: 99%

Caveolin-1 is enriched in the peroxisomal membrane of rat hepatocytes

et al. 2009

View full text Add to dashboard Cite

Caveolae are a subtype of cholesterol-enriched lipid microdomains/rafts that are routinely detected as vesicles pinching off from the plasma membrane. Caveolin-1 is an essential component of caveolae. Hepatic caveolin-1 plays an important role in liver regeneration and lipid metabolism. Expression of caveolin-1 in hepatocytes is relatively low, and it has been suggested to also reside at other subcellular locations than the plasma membrane. Recently, we found that the peroxisomal membrane contains lipid microdomains. Like caveolin-1, hepatic peroxisomes are involved in lipid metabolism. Here, we analyzed the subcellular location of caveolin-1 in rat hepatocytes. The subcellular location of rat hepatocyte caveolin-1 was analyzed by cell fractionation procedures, immunofluorescence, and immuno-electron microscopy. Green fluorescent protein (GFP)-tagged caveolin-1 was expressed in rat hepatocytes. Lipid rafts were characterized after Triton X-100 or Lubrol WX extraction of purified peroxisomes. Fenofibric acid-dependent regulation of caveolin-1 was analyzed. Peroxisome biogenesis was studied in rat hepatocytes after RNA interference-mediated silencing of caveolin-1 and caveolin-1 knockout mice. Cell fractionation and microscopic analyses reveal that caveolin-1 colocalizes with peroxisomal marker proteins (catalase, the 70 kDa peroxisomal membrane protein PMP70, the adrenoleukodystrophy protein ALDP, Pex14p, and the bile acid

show abstract

“…For example, fatty infiltration is a prominent morphological and biochemical feature ofearly liver regeneration, and is most conspicuous during the first 2 days following subtotal hepatectomy (Bucher and Malt, 1971). The composition of this material is mainly neutral lipid, and its source appears to be body fat (Bartsch and Gerber, 1966;Glende and Morgan, 1968). In addition, ultrastructural studies have demonstrated that at least some lipid enters regenerating hepatocytes by the process of pinocytosis (Trotter, 1965).…”

mentioning

confidence: 99%

Metabolism of 7,12,-dimethylbenz(a)anthracene by normal and regenerating rat livers

Tomsak¹,

Cook²

1977

Br J Cancer

View full text Add to dashboard Cite

Summary.-The in vitro metabolisms of [14C]7,12 -dimethylbenz(a)anthracene (DMBA) by post-mitochondrial supernates and microsomes from intact and regenerating rat livers were compared. Both cell fractions from regenerating livers at 48, 72, and 96 h after partial hepatectomy metabolized less [14C]DMBA than similar fractions from intact livers. Prior in vivo treatment with DMBA enhanced metabolism by the cell fractions from both groups, but specific activities of cell fractions from regenerating livers were always about 60% or less of those from intact livers. Thinlayer chromatographic analysis of metabolites formed in incubations using either cell fraction failed to reveal distinct differences between ether-soluble or water-soluble products of similar fractions from intact and regenerating livers. However, highly reproducible differences were found between chromatograms of water-soluble metabolites formed by microsomes and post-mitochondrial supernates in both intact and regenerating livers. Extrapolations from these studies indicate large differences in the metabolic capacity of intact and regenerating livers when expressed on a whole-liver basis, but it is suggested that there may be additional factors contributing to the increased retention of DMBA by regenerating livers.ACTIVELY dividing cells are more sensitive to chemical carcinogenesis than are non-dividing cells (Warwick, 1971;Weisburger and Williams, 1975). For example, regenerating rodent liver is more susceptible than intact liver to tumour induction by 7,12-dimethylbenz-(a)anthracene (DMBA) (Pound, 1968;Marquardt, Sternberg and Philips, 1970) and other chemical carcinogens (Chernozemski and Warwick, 1970;Craddock, 1973), but the reasons for this vulnerability are not known with certainty. In a comparison of [3H]DMBA uptake and persistence in intact and regenerating liver, we found that unmetabolized DMBA persisted at high levels in nuclei and other fractions from regenerating liver, but rapidly disappeared from intact liver; preliminary experiments demonstrated that hepatic microsomes from DMBAtreated partially hepatectomized animals metabolized less [14C]DMBA in vitro than did microsomes from DMBA-treated intact animals (Tomsak and Cook, 1975).We now report a more detailed quantitative comparison of DMBA metabolism, using microsomes and post-mitochondrial supernates prepared from intact and regenerating livers, and also report comparative analyses of DMBA metabolites, using thin-layer chromatography as a screening technique.

show abstract

Alteration in liver lipid and lipid fatty acid composition after partial hepatectomy in the rat

Cited by 52 publications

References 35 publications

Fatty acid synthesis in the regenerating liver of the rat

Fatty acid synthesis in the regenerating liver of the rat

Caveolin-1 is enriched in the peroxisomal membrane of rat hepatocytes

Metabolism of 7,12,-dimethylbenz(a)anthracene by normal and regenerating rat livers

Contact Info

Product

Resources

About