Alteration in IFN-γ and CCL2 serum levels at first trimester of pregnancy contribute to development of preeclampsia and fetal growth restriction

Vafaei, Homeira; Faraji, Saeideh; Ahmadi, Moslem; Tabei, Seyed Mohammad Bagher; Fereidoni, Solmaz; Shiravani, Zahra; Hosseini, Seyedeh Najme; Asadi, Nasrin; Kasraeian, Maryam; Faraji, Azam; Abbasi, Omid; Gharesi‐Fard, Behrouz

doi:10.1016/j.tjog.2022.09.005

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…Some studies have found that elevated concentrations of IFN-γ in the placenta may lead to PE progression by affecting trophoblast invasion, spiral artery remodeling, trophoblast cell apoptosis and placental angiogenesis [9,[33][34][35], while others have found no significant difference in IFN-γ levels between women with and without PE [36,37]. Another study investigating the association between serum levels of cytokines in the first trimester of pregnancy with the onset of PE found that the mean serum level of IFN-γ was significantly higher in the PE group compared to that of the healthy group [38]. In our study, no significant difference was observed between the intensity and extension of staining of IFN-γ in the placenta from controls and PE using the double immunofluorescence method.…”

Section: Discussionmentioning

confidence: 99%

Effect of Granzyme K, FasL and Interferon-γ Expression in Placentas with Preeclampsia

Vukoja,

Ćurlin,

Vukojević

et al. 2024

Biomedicines

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This study aimed to investigate the cytotoxic activity of decidual lymphocytes and the mRNA/protein expression of cytotoxic proteins in various cell types in the context of preeclampsia (PE) compared to those of healthy pregnancies. We analyzed fresh decidua basalis tissue and tissue embedded in paraffin (FFPE) from PE pregnancies (n = 15) and compared them with those of healthy pregnancies (n = 15) of the corresponding gestational age. Using double immunofluorescence staining, we observed differences in the intensity and distribution of staining for granzyme K (GZMK) and FasL in extravillous trophoblasts. RT-qPCR analysis of FFPE placental tissue showed that GZMK mRNA expression was statistically higher (p < 0.0001) in PE compared to that of healthy controls. On the contrary, there was a low expression (p < 0.001) of FasL mRNA in PE compared to controls, while there was no statistically significant difference for IFN-γ mRNA between PE and controls. Although the level of cytotoxic activity changed depending on the ratio of effector and target cells, there was no significant difference observed between PE and controls in this in vitro study. In conclusion, in PE, extravillous trophoblasts exhibited increased expression of GZMK and decreased expression of FasL. These changes may contribute to impaired trophoblast invasion. However, these alterations did not appear to affect the cytotoxic properties of decidual lymphocytes. Additionally, the possibility of cell sorter separation of decidual lymphocytes would greatly contribute to a better understanding of single cells’ genetic profiles.

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Section: Discussionmentioning

confidence: 99%

Effect of Granzyme K, FasL and Interferon-γ Expression in Placentas with Preeclampsia

Vukoja,

Ćurlin,

Vukojević

et al. 2024

Biomedicines

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show abstract

“…Most studies have shown that IFN-γ expression is increased in spontaneous abortion or recurrent pregnancy loss patients ( 46 – 48 ). Higher levels of IFN-γ were found in serum samples of women in first trimester of pregnancy who have experienced preeclampsia and fetal growth restriction ( 49 ). Therefore, it is considered that inhibiting and regulation of the immune system by immunomodulators or immunosuppressive agents could be an effective treatment strategy to resolve RIF ( 50 , 51 ).…”

Section: Discussionmentioning

confidence: 99%

Pro- and anti-inflammatory cytokines and growth factors in patients undergoing in vitro fertilization procedure treated with prednisone

Piekarska,

Dratwa,

Radwan

et al. 2023

Front. Immunol.

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Embryo implantation is a key moment in pregnancy. Abnormal production of pro- and anti-inflammatory cytokines, their receptors and other immune factors may result in embryo implantation failure and pregnancy loss. The aim of this study was to determine the profile of selected pro- and anti-inflammatory factors in the blood plasma of patients undergoing in vitro fertilization (IVF) and control women who achieved pregnancy after natural conception. The examined patients were administered steroid prednisone. We present results concern the plasma levels of IFN-ɣ, BDNF, LIF, VEGF-A, sTNFR1 and IL-10. We found that IVF patients receiving steroids differed significantly from patients who were not administered such treatment in terms of IFN-γ and IL-10 levels. Moreover, IVF patients differed in secretion of all tested factors with the fertile controls. Our results indicated that women who secrete at least 1409 pg/ml of sTNFR1 have a chance to become pregnant naturally and give birth to a child, while patients after IVF must achieve a concentration of 962.3 pg/ml sTNFR1 in blood plasma for successful pregnancy. In addition, IVF patients secreting VEGF-A above 43.28 pg/ml have a greater risk of miscarriage or a failed transfer in comparison to women secreting below this value. In conclusion, fertile women present a different profile of pro- and anti-inflammatory cytokines, and growth factors compared to patients with recurrent implantation failure (RIF).

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Do body height and shoe size of pregnant women influence the success of vaginal delivery for macrosomia?

Chang,

Yang,

Wang

2024

Taiwanese Journal of Obstetrics and Gynecology

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Alteration in IFN-γ and CCL2 serum levels at first trimester of pregnancy contribute to development of preeclampsia and fetal growth restriction

Cited by 4 publications

References 25 publications

Effect of Granzyme K, FasL and Interferon-γ Expression in Placentas with Preeclampsia

Effect of Granzyme K, FasL and Interferon-γ Expression in Placentas with Preeclampsia

Pro- and anti-inflammatory cytokines and growth factors in patients undergoing in vitro fertilization procedure treated with prednisone

Do body height and shoe size of pregnant women influence the success of vaginal delivery for macrosomia?

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