Alteration by thioredoxin f over-expression of primary carbon metabolism and its response to elevated CO2 in tobacco (Nicotiana tabacum L.)

Aranjuelo, Íker; Tcherkez, Guillaume; Jauregui,; Gilard, Françoise; Ancín, María; Millán, Alicia Fernández San; Larraya, Luis; Veramendi, Jon; Farrán, Inmaculada

doi:10.1016/j.envexpbot.2015.05.008

Cited by 10 publications

(8 citation statements)

References 30 publications

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“…We previously demonstrated that the overexpression of Trx f in tobacco chloroplasts has a positive effect on transitory starch accumulation and leaf biomass production [48,69,70]. Consistent with this, here we show a decrease in starch content in the corresponding Trx f silenced plants, which did not display any visible phenotype (Figures 3a and 4b).…”

Section: Starch Synthase As the Main Determinant Of Starch Accumulatisupporting

confidence: 91%

NTRC and Thioredoxin f Overexpression Differentially Induces Starch Accumulation in Tobacco Leaves

Ancín¹,

Larraya²,

Millán³

et al. 2019

Plants

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Thioredoxin (Trx) f and NADPH-dependent Trx reductase C (NTRC) have both been proposed as major redox regulators of starch metabolism in chloroplasts. However, little is known regarding the specific role of each protein in this complex mechanism. To shed light on this point, tobacco plants that were genetically engineered to overexpress the NTRC protein from the chloroplast genome were obtained and compared to previously generated Trx f-overexpressing transplastomic plants. Likewise, we investigated the impact of NTRC and Trx f deficiency on starch metabolism by generating Nicotiana benthamiana plants that were silenced for each gene. Our results demonstrated that NTRC overexpression induced enhanced starch accumulation in tobacco leaves, as occurred with Trx f. However, only Trx f silencing leads to a significant decrease in the leaf starch content. Quantitative analysis of enzyme activities related to starch synthesis and degradation were determined in all of the genotypes. Zymographic analyses were additionally performed to compare the amylolytic enzyme profiles of both transplastomic tobacco plants. Our findings indicated that NTRC overexpression promotes the accumulation of transitory leaf starch as a consequence of a diminished starch turnover during the dark period, which seems to be related to a significant reductive activation of ADP-glucose pyrophosphorylase and/or a deactivation of a putative debranching enzyme. On the other hand, increased starch content in Trx f-overexpressing plants was connected to an increase in the capacity of soluble starch synthases during the light period. Taken together, these results suggest that NTRC and the ferredoxin/Trx system play distinct roles in starch turnover.

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Section: Starch Synthase As the Main Determinant Of Starch Accumulatisupporting

confidence: 91%

NTRC and Thioredoxin f Overexpression Differentially Induces Starch Accumulation in Tobacco Leaves

Ancín¹,

Larraya²,

Millán³

et al. 2019

Plants

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“…Contrastingly, increases in biomass were not reported for field-grown plants, although Trx f -overexpressing plants still displayed increased specific leaf weight (>20% compared with the WT), and increases in both starch and soluble sugars of up to 3.6- and 1.7-fold, respectively (Farran et al , 2014). The increase in starch level was also mantained under high CO 2 conditions (Aranjuelo et al , 2015). Despite these changes, this manipulation did not lead to detectable increases in photosynthetic rates under current CO 2 levels, and a decrease in J max was reported under elevated CO 2 .…”

Section: Improving the Efficiency Of Responses To The Fluctuating Ligmentioning

confidence: 85%

“…A relatively unexplored strategy for increasing photosynthetic carbon assimilation is by directly targeting Trx with the aim of modulating the redox regulation of the Trx-regulated enzymes more rapidly. As reviewed recently by Nikkanen et al (2017), overexpression of Trx f or NTRC (Table 3) has been suggested as a viable strategy for increasing productivity, Increased levels of TRX f in tobacco have been shown to lead to increases in specific leaf weight, starch, and sugars under both ambient and increased CO 2 conditions (Sanz-Barrio et al , 2013; Farran et al , 2014; Aranjuelo et al , 2015). Under glasshouse conditions, overexpression of Trx f has led to an increase of 1.7-fold in biomass and up to 5.5 times the amount of fermentable carbohydrates, specifically seven times the amount of starch and twice as much sucrose is present in the WT at the end of the growth cycle (Sanz-Barrio et al , 2013).…”

Section: Improving the Efficiency Of Responses To The Fluctuating Ligmentioning

confidence: 99%

Feeding the world: improving photosynthetic efficiency for sustainable crop production

Simkin

López-Calcagno

Raines

2019

Journal of Experimental Botany

349

239

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“…Relative metabolite content was determined by gas chromatography coupled to time-of-flight mass spectrometry (GC-MS) using a LECO Pegasus III coupled to an Agilent 6890N GC system. Sample derivatization and GC-MS analyses were carried out as described in Aranjuelo et al (2015) . Peak identity was established by comparison of the fragmentation pattern with MS-available databases (NIST).…”

Section: Methodsmentioning

confidence: 99%

“…Together and closely related with these physiological responses, plant metabolome is also perturbed under e[CO 2 ] ( Aranjuelo et al, 2015 ; Tcherkez et al, 2020 ). The metabolome of the plant is the link between genotype and phenotype, allowing to study changes in gene expression in response to the environment ( Saito and Matsuda, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Soybean Inoculated With One Bradyrhizobium Strain Isolated at Elevated [CO2] Show an Impaired C and N Metabolism When Grown at Ambient [CO2]

et al. 2021

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Soybean (Glycine max L.) future response to elevated [CO2] has been shown to differ when inoculated with B. japonicum strains isolated at ambient or elevated [CO2]. Plants, inoculated with three Bradyrhizobium strains isolated at different [CO2], were grown in chambers at current and elevated [CO2] (400 vs. 700 ppm). Together with nodule and leaf metabolomic profile, characterization of nodule N-fixation and exchange between organs were tested through 15N2-labeling analysis. Soybeans inoculated with SFJ14-36 strain (isolated at elevated [CO2]) showed a strong metabolic imbalance, at nodule and leaf levels when grown at ambient [CO2], probably due to an insufficient supply of N by nodules, as shown by 15N2-labeling. In nodules, due to shortage of photoassimilate, C may be diverted to aspartic acid instead of malate in order to improve the efficiency of the C source sustaining N2-fixation. In leaves, photorespiration and respiration were boosted at ambient [CO2] in plants inoculated with this strain. Additionally, free phytol, antioxidants, and fatty acid content could be indicate induced senescence due to oxidative stress and lack of nitrogen. Therefore, plants inoculated with Bradyrhizobium strain isolated at elevated [CO2] may have lost their capacity to form effective symbiosis at ambient [CO2] and that was translated at whole plant level through metabolic impairment.

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Alteration by thioredoxin f over-expression of primary carbon metabolism and its response to elevated CO2 in tobacco (Nicotiana tabacum L.)

Cited by 10 publications

References 30 publications

NTRC and Thioredoxin f Overexpression Differentially Induces Starch Accumulation in Tobacco Leaves

NTRC and Thioredoxin f Overexpression Differentially Induces Starch Accumulation in Tobacco Leaves

Feeding the world: improving photosynthetic efficiency for sustainable crop production

Soybean Inoculated With One Bradyrhizobium Strain Isolated at Elevated [CO2] Show an Impaired C and N Metabolism When Grown at Ambient [CO2]

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