Altemicidin, a new acaricidal and antitumor substance. I. Taxonomy, fermentation, isolation and physico-chemical and biological properties.

Abstract

Screening of new insecticidal and acaricidal antibiotics was carried out with reference to anti-brine shrimp activity from actinomycete strains isolated from marine environments. Of 200 actinomycete isolates, one isolate was found to produce a newsubstance, altemicidin. The strain was isolated from sea mudcollected at Gamo, Miyagi Prefecture, Japan, and identified as Streptomyces sioyaensis SA-1758. Altemicidin was purified by Diaion CHP-20P and Sephadex LH-20 column chromatographies. The molecular formula was determined as C13H20N4O7Sby elemental analysis, MSand 13C NMR spectrum. Altemicidin showed not only acaricidal activity but also antitumor activity. The compound showed no antimicrobial activity except the inhibitory activity to Xanthomonas strains.

Ocean Dwelling Actinobacteria as Source of Antitumor Compounds

“…The strain SA-1758 produced a monoterpene-alkaloid, altemicidin (Takahashi et al 1989a). The compound strongly inhibited the growth of L1210 and carcinoma IMC cell lines with IC50 values of 0.84 and 0.82 μg/mL, respectively (Takahashi et al 1989b). Neomarinones are sesquiterpenoid naphtho-quinones with a mixed polyketide-terpenoid origin (Pathirana et al 1992).…”

Electrospray Ionization Mass Spectra of Piperazimycins A and B and γ-Butyrolactones from a Marine-derived Streptomyces sp.†

“…The cytotoxic assay was performed according to Takahashi et al [29] at concentrations of 10 mg/ml with actinomycin D (1.0 mg/well) for comparison.…”

Agroactive Compounds of Microbial Origin

“…In this approach, improved or modified screening methods are critical to obtaining better compounds. New compounds thus discovered are jietacin (112), discovered by using the pine wood nematode; altemicidin (138) and dioxapyrrolomycin (20), detected using brine shrimp; and allosamidin (130), found as an enzyme inhibitor for chitinase. This category also includes a new herbicidal inhibitor of starch synthesis, 6241-B substance (67).…”