alphavbeta3 integrin expression and pinopod formation in normal and out-of-phase endometria of fertile and infertile women

Creus, Montserrat; Ordi, Jaume; Fábregues, Francisco; Casamitjana, Roser; Ferrer, Berta; Coll, Elisenda; Vanrell, Juan A.; Balasch, Juan

doi:10.1093/humrep/17.9.2279

Cited by 59 publications

(42 citation statements)

References 33 publications

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“…Digital micrographs were taken with the computer program Quartz PCI (Quartz Imaging Co., Vancouver, BC, Canada), and were evaluated independently by two observers. As previously reported by others and ourselves (13,21,34), pinopodes were defined as spherical protrusions without microvilli on the apical surface of the luminal uterine endometrium and were semiquantitatively evaluated as absent (0), isolated pinopodes (+), small groups of pinopodes (++), and confluent pinopodes (+ + +) (Fig. 2).…”

Section: Scanning Electron Microscopymentioning

confidence: 98%

“…In all women, basal body temperature, luteal serum concentrations of estradiol and progesterone, and endometrial biopsies were used in the same cycle to assess luteal function according to a scheme of evaluation previously reported (17,21). Commencing on days 8-10 of the study cycle (depending on the cycle length of the woman) patients underwent daily transvaginal ultrasonographic evaluation of the follicular growth using a 5 MHz vaginal transducer attached to an Aloka scanner (Model SSD-620; Aloka Co. Ltd, Tokyo, Japan).…”

Section: Patients and Study Cyclementioning

confidence: 99%

“…Immunohistochemistry αvβ3 integrin was detected in frozen sections using the EnVision system (Dako Co., Carpinteria, CA, USA) as previously reported (17,21,26). Briefly, 4 µm sections were fixed 10 min in acetone at 4 • C and dried.…”

Section: Endometrial Datingmentioning

confidence: 99%

“…Then the slides were incubated with the primary antibody for 40 min and washed in TBS (Dako). The monoclonal antibody LM609 (Chemicon, Temecula, CA, USA, dilution 1:200), which recognizes the complete αvβ3 heterodimer (27) and is being widely applied by us (17,21,28,29) and others (14,(30)(31)(32) was used. The peroxidase labelled polymer was then applied for 40 min.…”

Section: Endometrial Datingmentioning

confidence: 99%

“…The intensity of staining of the endometrial components was evaluated by a semiquantitative scoring system (0-3) as follows (17,21,27,28): absent (0), weak or focal (+), moderate (++), and strong (+ + +) (Fig. 1).…”

Section: Endometrial Datingmentioning

confidence: 99%

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Endometrial Pinopode and αv 3 Integrin Expression Is Not Impaired in Infertile Patients with Endometriosis

Ordi

Creus

Casamitjana

et al. 2003

J Assist Reprod Genet

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Purpose : To investigate endometrial receptivity in terms of pinopode formation and αvβ3 integrin expression in infertile women with endometriosis during natural cycles. Methods : We investigated the expression of αvβ3 integrin and pinopode formation in the endometrium of 12 infertile patients with stage I or II endometriosis as the only cause of infertility, 12 infertile patients having unexplained infertility, and 12 fertile women who were undergoing tubal sterilization. Two endometrial biopsies (postovulatory day +7 to +8 and 4 days later) were performed during a single menstrual cycle in each subject. Results : No statistically significant difference regarding αvβ3 integrin expression and pinopode formation was found between infertile patients with endometriosis and the two control groups. Conclusion : αvβ3 integrin expression and pinopode formation are not reduced during the window of implantation in patients with stage I-II endometriosis. Whether these results imply normal endometrial receptivity in such patients or add to the increasing uncertainty about the clinical value of assessing the endometrium with those markers of implantation, warrants further studies.

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Section: Scanning Electron Microscopymentioning

confidence: 98%

Section: Patients and Study Cyclementioning

confidence: 99%

Section: Endometrial Datingmentioning

confidence: 99%

Section: Endometrial Datingmentioning

confidence: 99%

Section: Endometrial Datingmentioning

confidence: 99%

See 3 more Smart Citations

Endometrial Pinopode and αv 3 Integrin Expression Is Not Impaired in Infertile Patients with Endometriosis

Ordi

Creus

Casamitjana

et al. 2003

J Assist Reprod Genet

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Endometrial receptivity: Clinical assessment in relation to fertility, infertility, and antifertility

Makker

Singh

2006

Med. Res. Rev.

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Fertility in humans and other mammalian species depends absolutely on synchronous events that render the developing blastocyst and the receiving uterus competent for implantation. Endometrial receptivity is defined as the period during which the endometrial epithelium acquires functional, but transient, ovarian steroid-dependent status supportive to blastocyst acceptance and implantation. Once inside the uterus, the blastocyst is surrounded by an intact luminal epithelium, which is considered to act as barrier to its attachment, except for this short period of high endometrial receptivity to blastocyst signal(s). Its transport and permeability properties, in conjunction with cellular action of the endometrium and the embryo, have been suggested to influence creation and maintenance of informational and nutritional status of uterine luminal milieu. This period, also termed as the 'window of implantation,' is limited to days 20-24 of menstrual cycle in humans. However, establishment of endometrial receptivity is still a biological mystery that remains unsolved despite marked advances in our understanding of endometrial physiology following extensive research associated with its development and function. This review deals with various structural, biochemical, and molecular events in the endometrium coordinated within the implantation window that constitute essential elements in the repertoire that signifies endometrial receptivity and is aimed to achieve a better understanding of its relationship to fertility, infertility, and for the development of targeted antifertility agents for human use and welfare.

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Assessment of Uterine Receptivity

Çakmak

Taylor

2012

Practical Manual of in Vitro Fertilization

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alphavbeta3 integrin expression and pinopod formation in normal and out-of-phase endometria of fertile and infertile women

Abstract: There is a clear dissociation in the temporal expression of the most cited markers postulated to frame the window of implantation. The functional significance (if any) of these new markers remains to be established.

Cited by 59 publications

References 33 publications

Endometrial Pinopode and αv 3 Integrin Expression Is Not Impaired in Infertile Patients with Endometriosis

Endometrial Pinopode and αv 3 Integrin Expression Is Not Impaired in Infertile Patients with Endometriosis

Endometrial receptivity: Clinical assessment in relation to fertility, infertility, and antifertility

Assessment of Uterine Receptivity

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