Alphafold-multimer predicts cross-kingdom interactions at the plant-pathogen interface

Homma, Felix; Huang, Jie; Hoorn, Renier A. L. van der

doi:10.1101/2023.04.03.535425

Cited by 3 publications

(1 citation statement)

References 76 publications

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“…In addition, P. infestans secretes the Kazal-like protease inhibitors EPI1 and EPI10 that were shown to bind and inhibit P69B (Gupta et al, 2015;Paulus et al, 2020;Tian et al, 2004Tian et al, , 2005. The recent identification of P69B inhibitors in Cladosporium fulvum, Fusarium oxysporum and Xanthomonas perforans suggests that inactivation of P69B may be a general virulence strategy of diverse microbial pathogens (Homma et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

The tomato P69 subtilase family is involved in resistance to bacterial wilt

Zhang,

Planas‐Marquès,

Mazier

et al. 2023

The Plant Journal

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SUMMARYThe intercellular space or apoplast constitutes the main interface in plant–pathogen interactions. Apoplastic subtilisin‐like proteases—subtilases—may play an important role in defence and they have been identified as targets of pathogen‐secreted effector proteins. Here, we characterise the role of the Solanaceae‐specific P69 subtilase family in the interaction between tomato and the vascular bacterial wilt pathogen Ralstonia solanacearum. R. solanacearum infection post‐translationally activated several tomato P69s. Among them, P69D was exclusively activated in tomato plants resistant to R. solanacearum. In vitro experiments showed that P69D activation by prodomain removal occurred in an autocatalytic and intramolecular reaction that does not rely on the residue upstream of the processing site. Importantly P69D‐deficient tomato plants were more susceptible to bacterial wilt and transient expression of P69B, D and G in Nicotiana benthamiana limited proliferation of R. solanacearum. Our study demonstrates that P69s have conserved features but diverse functions in tomato and that P69D is involved in resistance to R. solanacearum but not to other vascular pathogens like Fusarium oxysporum.

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Section: Introductionmentioning

confidence: 99%

The tomato P69 subtilase family is involved in resistance to bacterial wilt

Zhang,

Planas‐Marquès,

Mazier

et al. 2023

The Plant Journal

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Multifaceted roles of plant glycosyl hydrolases during pathogen infections: more to discover

Sivaramakrishnan,

Veeraganti Naveen Prakash,

Chandrasekar

2024

Planta

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Zinc-finger (ZiF) fold secreted effectors form a functionally diverse family across lineages of the blast fungusMagnaporthe oryzae

Concepcion,

Langner,

Fujisaki

et al. 2023

Preprint

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Filamentous plant pathogens deliver effector proteins into host cells to suppress host defence responses and manipulate metabolic processes to support colonization. Understanding the evolution and molecular function of these effectors provides knowledge about pathogenesis and can suggest novel strategies to reduce damage caused by pathogens. However, effector proteins are highly variable, share weak sequence similarity and, although they can be grouped according to their structure, only a few structurally conserved effector families have been functionally characterized to date. Here, we demonstrate that Zinc-finger fold (ZiF) secreted proteins form a functionally diverse effector family in the blast fungus Magnaporthe oryzae. This family relies on the Zinc-finger motif for protein stability and is ubiquitously present, forming different effector tribes in blast fungus lineages infecting 13 different host species. Homologs of the canonical ZiF effector, AVR-Pii from rice infecting isolates, are present in multiple M. oryzae lineages, and the wheat infecting strains of the fungus, for example, possess an allele that also binds host Exo70 proteins and activates the immune receptor Pii. Furthermore, ZiF tribes vary in the host Exo70 proteins they bind, indicating functional diversification and an intricate effector/host interactome. Altogether, we uncovered a new effector family with a common protein fold that has functionally diversified in lineages of M. oryzae. This work expands our understanding of the diversity of M. oryzae effectors, the molecular basis of plant pathogenesis and may ultimately facilitate the development of new sources for pathogen resistance.

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Alphafold-multimer predicts cross-kingdom interactions at the plant-pathogen interface

Cited by 3 publications

References 76 publications

The tomato P69 subtilase family is involved in resistance to bacterial wilt

The tomato P69 subtilase family is involved in resistance to bacterial wilt

Multifaceted roles of plant glycosyl hydrolases during pathogen infections: more to discover

Zinc-finger (ZiF) fold secreted effectors form a functionally diverse family across lineages of the blast fungusMagnaporthe oryzae

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