Alpha-Thalassemia in North Morocco: Prevalence and Molecular Spectrum

Laghmich, Achraf; Ismaili, Fatima Zahra Alaoui; Barakat, Amina; Nourouti, Naima Ghailani; Khattab, Mohamed; Mechita, Mohcine Bennani

doi:10.1155/2019/2080352

Cited by 11 publications

(9 citation statements)

References 43 publications

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“…This difference in distribution of the disease forms is often found in the small geographic areas from different countries (11). Our results are in agreement with those of Agouzal et al and Laghmich et al suggesting Laouamra as a hotspot of hemoglobinopathies in the country (6,7). The concentration of patients in this area could be a result of three major risk factors: i) its proximity to Kenitra province; the most touched region reported in Morocco (12,13).…”

Section: Discussionsupporting

confidence: 90%

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Pattern of Hemoglobinopathies: A Cross-sectional Study in North of Morocco

Ismaili

Laghmich

Nourouti

et al. 2020

Preprint

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Background: Hemoglobinopathies are the most frequent widely spread genetic disorders. In Morocco, epidemiological and clinical data are scarce. The present study aims to determine the spectrum and geographic distribution of hemoglobinopathies in Larache province, North of Morocco.Methods: A retrospective cross-sectional study was conducted from January 2015 to December 2018 at the provincial hospital of Larache city. All patients’ records having a hemoglobinopathy were analyzed. Background data (age, gender, and origin) of each case were analyzed. Results: Our study showed an overall frequency of hemoglobinopathies of 3.6%. Sickle cell trait was registered to be the most common disorder in our studied population (42.3%), followed by β-thalassemia Trait (20.2%), sickle cell disease (19.8%), major β-thalassemia (9.8%) and sickle/β-thalassemia (8%). The average age of subjects with hemoglobin disorders was 17.5 years. The majority of the patients (66.5%) were less than 20 years old. A disparity in the diseases geographic distribution was observed, 74.6% of patients came from rural areas. Conclusions: The present study is the first of its kind offering comprehensive data on hemoglobinopathies pattern in Northern Morocco. Our work may lay the foundation for screening programs for better prevention and coverage.

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Section: Discussionsupporting

confidence: 90%

“…Scarce studies reported the Northwest region as a geographic hotspot for hemoglobinopathies (6,7). The present work aims to determine the diseases patterns, geographic distribution, and their epidemiological characteristics in Larache province.…”

Section: Introductionmentioning

confidence: 96%

Pattern of Hemoglobinopathies: A Cross-sectional Study in North of Morocco

Ismaili

Laghmich

Nourouti

et al. 2020

Preprint

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“…Recent researches have highlighted a high prevalence of this group of inherited hemoglobin disorders in the studied region. Their severity and disabling nature make them a major public health problem [8].…”

Section: Introductionmentioning

confidence: 99%

Hemoglobinopathies in the North of Morocco: Consanguinity Pilot Study

Laghmich

Ismaili

Zian

et al. 2019

BioMed Research International

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Consanguinity is a social behavior characterized by the arrangement of marriages between relatives. It coincides generally with the geographic distribution of recessive genetic diseases as it increases the likelihood of homozygosis and, consequently, the incidence of their pathologies in the population. In this pilot study, we assess the effect of inbreeding on the burden of hemoglobinopathies in Northern Morocco. From January 2016 to December 2018, 197 children born in the studied region to three ancestral generations and diagnosed with hemoglobinopathies were subject to investigation. The rate of consanguinity in the parents' generation of children with hemoglobinopathies was 50.25%, with first cousin marriages accounting for 68.69% of consanguineous unions (FI = 0.02). The corresponding rates in the general population, based on a sample of N = 900, were 29.67% and 82.02%, respectively. The marriages between first cousins are the most common among the other types of consanguineous unions. Our study propounds that consanguinity substantially contributes to the hemoglobinopathy burden in the studied region and has changed little over time. Refraining from consanguineous marriages and detecting couples at risk could contribute to the reduction of the incidence of genetic diseases in our country.

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“…16,17 Additionally, LAMP assays were used to detect the haptoglobin gene deletion in alkaline-denatured blood. 18 In this study, we found that the target sequence was successfully amplified and that the wild type and target could be distinguished. LAMP assays for positive samples of thalassemia reached a plateau before 60 minutes, whereas the negative control samples entered the plateau after 70 minutes or showed no amplification.…”

Section: Discussionmentioning

confidence: 76%

<p>Establishment and Evaluation of a Novel Method Based on Loop-Mediated Isothermal Amplification for the Rapid Diagnosis of Thalassemia Genes</p>

Lin¹,

Li²,

Huang³

et al. 2020

RMHP

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Currently, thalassemia is commonly detected using gap-polymerase chain reaction (PCR) and deoxyribonucleic acid (DNA) reverse dot blot, which have high requirements of space, instruments, and personnel. Therefore, it is necessary to develop a new method for thalassemia detection with high sensitivity, low cost, and simple and fast operation. In this study, we aimed to design and evaluate a new method for detecting three α-thalassemia genes including-Southeast Asian (SEA),-α3.7, and-α4.2 and five β-thalassemia genes including 654M, 41/42M, −28M, 17M, and 27/28M based on loop-mediated isothermal amplification (LAMP). Methods: Primer sequences were designed using Primer Explorer V4 software. Blood samples (5 mL) were collected from all participants in EDTA. DNA was extracted using Chelex 100 and was subjected to LAMP. LAMP products were detected by fluorescence development in ultraviolet light. Results: We found that LAMP assays for positive samples of thalassemia reached a plateau before 60 minutes, whereas the negative control samples entered the plateau after 70 minutes or showed no amplification. The concentration range of positive reactions was between 20-60 pg/μL and 20-60 ng/μL. Additionally, there were no cross-reactivities among 8 thalassemia subtypes. For clinical samples, the positive sample tube showed strong green fluorescence, whereas the negative tube showed light green fluorescence. According to these results, the LAMP method has high sensitivity for detecting thalassemia (252/254). However, 43 false-positive results were obtained in the LAMP test. The LAMP assay was also of low cost and with simple and fast operation. Conclusion: The novel LAMP assay can be completed within 60 min using a heating block or a water bath, and the result can be read visually based on color change to detect thalassemia. The LAMP assay fulfills the requirements of field application and resourcelimited areas, especially those with primary hospitals and rural areas.

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Alpha-Thalassemia in North Morocco: Prevalence and Molecular Spectrum

Cited by 11 publications

References 43 publications

Pattern of Hemoglobinopathies: A Cross-sectional Study in North of Morocco

Pattern of Hemoglobinopathies: A Cross-sectional Study in North of Morocco

Hemoglobinopathies in the North of Morocco: Consanguinity Pilot Study

<p>Establishment and Evaluation of a Novel Method Based on Loop-Mediated Isothermal Amplification for the Rapid Diagnosis of Thalassemia Genes</p>

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